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Rabbit Polyclonal USP39 antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human USP39 aa 350-400.

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Images

Immunoprecipitation - Anti-USP39 antibody (AB245571), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Synthetic Peptide within Human USP39 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q53GS9

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IP
Human
Tested
Mouse
Predicted
Orangutan
Predicted

Tested
Tested

Species
Human
Dilution info
2.00000-10.00000 µg/mg of lysate
Notes

-

Predicted
Predicted

Species
Mouse, Orangutan
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Deubiquitinating enzyme that plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development (PubMed:33127822, PubMed:34614178). Plays a role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the precatalytic spliceosome (PubMed:11350945, PubMed:26912367). Specifically regulates immunoglobulin gene rearrangement in a spliceosome-dependent manner, which involves modulating chromatin interactions at the Igh locus and therefore plays an essential role in B-cell development (By similarity). Regulates AURKB mRNA levels, and thereby plays a role in cytokinesis and in the spindle checkpoint (PubMed:18728397). Regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2 (PubMed:30771428). Plays also an important role in DNA repair by controlling the recruitment of XRCC4/LIG4 to DNA double-strand breaks for non-homologous end-joining repair (PubMed:34614178). Participates in antiviral activity by affecting the type I IFN signaling by stabilizing STAT1 and decreasing its 'Lys-6'-linked ubiquitination (PubMed:33127822). Contributes to non-canonical Wnt signaling during epidermal differentiation (By similarity). Acts as a negative regulator NF-kappa-B activation through deubiquitination of 'Lys-48'-linked ubiquitination of NFKBIA (PubMed:36651806).

Alternative names

CGI-21, HSPC332, PRO2855, USP39, Ubiquitin carboxyl-terminal hydrolase 39, SAD1 homolog, U4/U6.U5 tri-snRNP-associated 65 kDa protein

Recommended products

Rabbit Polyclonal USP39 antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human USP39 aa 350-400.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Synthetic Peptide within Human USP39 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q53GS9
Purification technique
Affinity purification Immunogen
Concentration
Loading...
Purification notes

ab245571 was affinity purified using an epitope specific to USP39 immobilized on solid support.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

USP39 also known as U4/U6.U5 tri-snRNP-associated protein or Sad1 homologue functions as a deubiquitinating enzyme which is essential for the ubiquitin-proteasome system in cells. This protein has a molecular mass of approximately 65 kDa and is expressed abundantly in the nucleus. It participates in the regulation of pre-mRNA splicing by facilitating the assembly of the spliceosome an intricate molecular machine.

Biological function summary

USP39 plays a significant role in pre-mRNA splicing a process critical for the maturation of messenger RNA. It forms part of the U4/U6.U5 tri-snRNP complex which is essential for spliceosome assembly. This protein ensures proper spliceosome cycling by stabilizing snRNPs contributing to the accurate removal of introns from pre-mRNA sequences. Its involvement in RNA processing highlights its importance in gene expression regulation.

Pathways

USP39 integrates into the spliceosome pathway and is also linked to the mRNA surveillance pathway. These pathways are important for correct mRNA synthesis and degradation. USP39 functions alongside other splicing factors such as PRPF8 and EFTUD2 which are important components of the spliceosome machinery. By working synchronously with these proteins it maintains efficient RNA processing and cellular homeostasis.

Associated diseases and disorders

USP39 has associations with various cancer types including breast and prostate cancer. Alterations in its expression or function can disrupt normal splicing and gene expression contributing to oncogenesis. In breast cancer for instance USP39 interacts with the BRCA1 protein influencing pathways linked to DNA repair and tumor suppression. In prostate cancer defects in its deubiquitination activity can lead to aberrant cell cycles and resistance to apoptosis highlighting its role in maintaining cellular health.

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1 product image

  • Immunoprecipitation - Anti-USP39 antibody (ab245571), expandable thumbnail

    Immunoprecipitation - Anti-USP39 antibody (ab245571)

    USP39 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

    ab245571 used for IP at 6 μg per reaction. For WB 1 μg/ml.

    Lane 1: ab245571 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Chemiluminescence detection: 30 seconds.

    All lanes: Immunoprecipitation - Anti-USP39 antibody (ab245571)

    Predicted band size: 65 kDa

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Product protocols

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