AB131244

Anti-USP39 antibody [EPR8683]

RabMAb
Recombinant
What is this?

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(19 Publications)

Rabbit Recombinant Monoclonal USP39 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 19 publications.

View Alternative Names

CGI-21, HSPC332, PRO2855, USP39, Ubiquitin carboxyl-terminal hydrolase 39, SAD1 homolog, U4/U6.U5 tri-snRNP-associated 65 kDa protein

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP39 antibody [EPR8683] (AB131244)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP39 antibody [EPR8683] (AB131244)

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labelling USP39 with ab131244 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody [EPR8683] (AB131244)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody [EPR8683] (AB131244)

Immunofluorescence analysis of HeLa cells labelling USP39 with ab131244 at 1/100 dilution.

Unknown

Western blot - Anti-USP39 antibody [EPR8683] (AB131244)

All lanes:

Western blot - Anti-USP39 antibody [EPR8683] (ab131244) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 65 kDa

false

OI-RD Scanning - Anti-USP39 antibody [EPR8683] (AB131244)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-USP39 antibody [EPR8683] (AB131244)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-USP39 antibody [EPR8683] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8683

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP39 also known as U4/U6.U5 tri-snRNP-associated protein or Sad1 homologue functions as a deubiquitinating enzyme which is essential for the ubiquitin-proteasome system in cells. This protein has a molecular mass of approximately 65 kDa and is expressed abundantly in the nucleus. It participates in the regulation of pre-mRNA splicing by facilitating the assembly of the spliceosome an intricate molecular machine.

Biological function summary

USP39 plays a significant role in pre-mRNA splicing a process critical for the maturation of messenger RNA. It forms part of the U4/U6.U5 tri-snRNP complex which is essential for spliceosome assembly. This protein ensures proper spliceosome cycling by stabilizing snRNPs contributing to the accurate removal of introns from pre-mRNA sequences. Its involvement in RNA processing highlights its importance in gene expression regulation.

Pathways

USP39 integrates into the spliceosome pathway and is also linked to the mRNA surveillance pathway. These pathways are important for correct mRNA synthesis and degradation. USP39 functions alongside other splicing factors such as PRPF8 and EFTUD2 which are important components of the spliceosome machinery. By working synchronously with these proteins it maintains efficient RNA processing and cellular homeostasis.

USP39 has associations with various cancer types including breast and prostate cancer. Alterations in its expression or function can disrupt normal splicing and gene expression contributing to oncogenesis. In breast cancer for instance USP39 interacts with the BRCA1 protein influencing pathways linked to DNA repair and tumor suppression. In prostate cancer defects in its deubiquitination activity can lead to aberrant cell cycles and resistance to apoptosis highlighting its role in maintaining cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deubiquitinating enzyme that plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development (PubMed : 33127822, PubMed : 34614178). Plays a role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the precatalytic spliceosome (PubMed : 11350945, PubMed : 26912367). Specifically regulates immunoglobulin gene rearrangement in a spliceosome-dependent manner, which involves modulating chromatin interactions at the Igh locus and therefore plays an essential role in B-cell development (By similarity). Regulates AURKB mRNA levels, and thereby plays a role in cytokinesis and in the spindle checkpoint (PubMed : 18728397). Regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2 (PubMed : 30771428). Also plays an important role in DNA repair by controlling the recruitment of XRCC4/LIG4 to DNA double-strand breaks for non-homologous end-joining repair (PubMed : 34614178). Participates in antiviral activity by affecting the type I IFN signaling by stabilizing STAT1 and decreasing its 'Lys-6'-linked ubiquitination (PubMed : 33127822). Contributes to non-canonical Wnt signaling during epidermal differentiation (By similarity). Acts as a negative regulator NF-kappa-B activation through deubiquitination of 'Lys-48'-linked ubiquitination of NFKBIA (PubMed : 36651806).

See full target information USP39

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:564 PubMed40715045

2025

The deubiquitinase USP24 suppresses ferroptosis in triple-negative breast cancer by stabilizing DHODH protein.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yang,Xiaoqin An,Shangzhu Yang,Xiaowen Lin,Ziyuan Chen,Qian Xue,Xi Chen,Yuan Wang,Ding Yan,Shirui Chen,Yuqing Fan,Daolin Tang,Wenfeng Yu,Jinbao Liu,Xin Chen

Cell biology international 49:1003-1014 PubMed40405439

2025

USP39 Depletion Plays Repressive Roles in Laryngeal Squamous Cell Carcinoma Development.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Xie,Lei Zhang,Xiaoyan Hu,Yahua Zhong,Zheng Li

Journal of translational medicine 21:807 PubMed37957720

2023

USP39 interacts with SIRT7 to promote cervical squamous cell carcinoma by modulating autophagy and oxidative stress via FOXM1.

Applications

Unspecified application

Species

Unspecified reactive species

Juanpeng Yu,Shuai Yuan,Jinglin Song,Shengsheng Yu

Nature communications 14:7032 PubMed37923718

2023

Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Donghai Cui,Zixiang Wang,Qianli Dang,Jing Wang,Junchao Qin,Jianping Song,Xiangyu Zhai,Yachao Zhou,Ling Zhao,Gang Lu,Hongbin Liu,Gang Liu,Runping Liu,Changshun Shao,Xiyu Zhang,Zhaojian Liu

Translational oncology 34:101713 PubMed37302347

2023

USP39-mediated deubiquitination of Cyclin B1 promotes tumor cell proliferation and glioma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Xiao,Xinyi Chen,Weiwei Hu,Wenjing Ma,Qianqian Di,Haimei Tang,Xibao Zhao,Guodong Huang,Weilin Chen

Cell death & disease 14:63 PubMed36707504

2023

USP39 stabilizes β-catenin by deubiquitination and suppressing E3 ligase TRIM26 pre-mRNA maturation to promote HCC progression.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Wang,Yongbin Lei,Gongye Zhang,Xiaomei Li,Jiahui Yuan,Tingting Li,Wei Zhong,Yuqi Zhang,Xuemei Tan,Gang Song

Journal of hematology & oncology 15:112 PubMed35978332

2022

The LINC00623/NAT10 signaling axis promotes pancreatic cancer progression by remodeling ac4C modification of mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Zengyu Feng,Kexian Li,Kai Qin,Juyong Liang,Minmin Shi,Yang Ma,Shiwei Zhao,Huaiyu Liang,Dongni Han,Baiyong Shen,Chenghong Peng,Hao Chen,Lingxi Jiang

Frontiers in pharmacology 13:858901 PubMed35600879

2022

ARV-771 Acts as an Inducer of Cell Cycle Arrest and Apoptosis to Suppress Hepatocellular Carcinoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanfei Deng,Cuifu Yu,Lushi Chen,Xin Zhang,Qiucheng Lei,Qing Liu,Gengxi Cai,Fang Liu

Genes 13: PubMed35627203

2022

The Deubiquitinase USP39 Promotes Esophageal Squamous Cell Carcinoma Malignancy as a Splicing Factor.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Zhu,Jianlin Ma,Minyi Lu,Zhihua Liu,Yongkun Sun,Hongyan Chen

Molecular oncology 16:388-404 PubMed33811456

2021

Ubiquitin-specific peptidase 39 promotes human glioma cells migration and invasion by facilitating ADAM9 mRNA maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Xiao,Wenjing Ma,Weiwei Hu,Qianqian Di,Xibao Zhao,Xingyu Ma,Xinyi Chen,Ping Sun,Han Wu,Zherui Wu,Weilin Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USP39 antibody [EPR8683]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP39 antibody [EPR8683] (AB131244)

Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody [EPR8683] (AB131244)

Western blot - Anti-USP39 antibody [EPR8683] (AB131244)

OI-RD Scanning - Anti-USP39 antibody [EPR8683] (AB131244)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise