AB131332

Anti-USP39 antibody [EPR8684]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Recombinant Monoclonal USP39 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

CGI-21, HSPC332, PRO2855, USP39, Ubiquitin carboxyl-terminal hydrolase 39, SAD1 homolog, U4/U6.U5 tri-snRNP-associated 65 kDa protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody [EPR8684] (AB131332)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody [EPR8684] (AB131332)

Immunofluorescent analysis of HeLa cells labelling USP39 with ab131332 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP39 antibody [EPR8684] (AB131332)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP39 antibody [EPR8684] (AB131332)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling USP39 with ab131332 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-USP39 antibody [EPR8684] (AB131332)

All lanes:

Western blot - Anti-USP39 antibody [EPR8684] (ab131332) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 65 kDa

false

Carrier free

Anti-USP39 antibody [EPR8684] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8684

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP39 also known as U4/U6.U5 tri-snRNP-associated protein or Sad1 homologue functions as a deubiquitinating enzyme which is essential for the ubiquitin-proteasome system in cells. This protein has a molecular mass of approximately 65 kDa and is expressed abundantly in the nucleus. It participates in the regulation of pre-mRNA splicing by facilitating the assembly of the spliceosome an intricate molecular machine.

Biological function summary

USP39 plays a significant role in pre-mRNA splicing a process critical for the maturation of messenger RNA. It forms part of the U4/U6.U5 tri-snRNP complex which is essential for spliceosome assembly. This protein ensures proper spliceosome cycling by stabilizing snRNPs contributing to the accurate removal of introns from pre-mRNA sequences. Its involvement in RNA processing highlights its importance in gene expression regulation.

Pathways

USP39 integrates into the spliceosome pathway and is also linked to the mRNA surveillance pathway. These pathways are important for correct mRNA synthesis and degradation. USP39 functions alongside other splicing factors such as PRPF8 and EFTUD2 which are important components of the spliceosome machinery. By working synchronously with these proteins it maintains efficient RNA processing and cellular homeostasis.

USP39 has associations with various cancer types including breast and prostate cancer. Alterations in its expression or function can disrupt normal splicing and gene expression contributing to oncogenesis. In breast cancer for instance USP39 interacts with the BRCA1 protein influencing pathways linked to DNA repair and tumor suppression. In prostate cancer defects in its deubiquitination activity can lead to aberrant cell cycles and resistance to apoptosis highlighting its role in maintaining cellular health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Deubiquitinating enzyme that plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development (PubMed : 33127822, PubMed : 34614178). Plays a role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the precatalytic spliceosome (PubMed : 11350945, PubMed : 26912367). Specifically regulates immunoglobulin gene rearrangement in a spliceosome-dependent manner, which involves modulating chromatin interactions at the Igh locus and therefore plays an essential role in B-cell development (By similarity). Regulates AURKB mRNA levels, and thereby plays a role in cytokinesis and in the spindle checkpoint (PubMed : 18728397). Regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2 (PubMed : 30771428). Plays also an important role in DNA repair by controlling the recruitment of XRCC4/LIG4 to DNA double-strand breaks for non-homologous end-joining repair (PubMed : 34614178). Participates in antiviral activity by affecting the type I IFN signaling by stabilizing STAT1 and decreasing its 'Lys-6'-linked ubiquitination (PubMed : 33127822). Contributes to non-canonical Wnt signaling during epidermal differentiation (By similarity). Acts as a negative regulator NF-kappa-B activation through deubiquitination of 'Lys-48'-linked ubiquitination of NFKBIA (PubMed : 36651806).

See full target information USP39

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:758 PubMed40264098

2025

Identification of USP39 as a prognostic and predictive biomarker for determining the response to immunotherapy in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Yuan,Beibei Xu,Yongcheng Su,Pingping Zhang,Xianbin Zhang,Peng Gong

Nature communications 14:7032 PubMed37923718

2023

Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Donghai Cui,Zixiang Wang,Qianli Dang,Jing Wang,Junchao Qin,Jianping Song,Xiangyu Zhai,Yachao Zhou,Ling Zhao,Gang Lu,Hongbin Liu,Gang Liu,Runping Liu,Changshun Shao,Xiyu Zhang,Zhaojian Liu

Frontiers in oncology 11:667495 PubMed34123832

2021

The Deubiquitinase USP39 Promotes ESCC Tumorigenesis Through Pre-mRNA Splicing of the mTORC2 Component Rictor.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhao,Huiwu Geng,Gang Liu,Qiang Ji,Xiaomin Cheng,Xinying Li,Wei Liu,Rick F Thorne,Renquan Zhang,Xiaoying Liu

Frontiers in cell and developmental biology 8:331 PubMed32457909

2020

The Use of Mononucleosome Immunoprecipitation for Analysis of Combinatorial Histone Post-translational Modifications and Purification of Nucleosome-Interacting Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Kashif Aziz Khan,Marlee K Ng,Peter Cheung

Oncogene 38:6414-6428 PubMed31332287

2019

RNA splicing factor USP39 promotes glioma progression by inducing TAZ mRNA maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Kaikai Ding,Jianxiong Ji,Xin Zhang,Bin Huang,Anjing Chen,Di Zhang,Xingang Li,Xinyu Wang,Jian Wang

Experimental and therapeutic medicine 13:1506-1513 PubMed28413501

2017

USP39 regulates the growth of SMMC-7721 cells via FoxM1.

Applications

Unspecified application

Species

Unspecified reactive species

Xianwen Yuan,Xitai Sun,Xiaolei Shi,Chunping Jiang,Decai Yu,Weiwei Zhang,Yitao Ding

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USP39 antibody [EPR8684]

Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody [EPR8684] (AB131332)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP39 antibody [EPR8684] (AB131332)

Western blot - Anti-USP39 antibody [EPR8684] (AB131332)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise