AB236453

Anti-USP39 antibody - N-terminal

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(2 Publications)

Rabbit Polyclonal USP39 antibody. N-terminal. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USP39.

View Alternative Names

CGI-21, HSPC332, PRO2855, USP39, Ubiquitin carboxyl-terminal hydrolase 39, SAD1 homolog, U4/U6.U5 tri-snRNP-associated 65 kDa protein

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody - N-terminal (AB236453)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody - N-terminal (AB236453)

Top panel : Flag -USP39 transfected U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for USP39 using ab236453 at a dilution of 1/2000 in ICC/IF.

Middle panel : Anti-Flag.

Bottom panel : Merged (+ DAPI) image.

Cells were fixed using 2% PFA. Incubated with primary antibody overnight at +4°C.

Flow Cytometry - Anti-USP39 antibody - N-terminal (AB236453)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-USP39 antibody - N-terminal (AB236453)

Flow Cytometry analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells, labeling USP39 with ab236453 at a 1/200 dilution (green) compared to a negative control (no primary antibody) (blue).

Cells were fixed in PFA 1% for 10 minutes, permeabilized with Triton X-100 and incubated with the primary antibody overnight.

Supplier Data

Immunoprecipitation - Anti-USP39 antibody - N-terminal (AB236453)

USP39 was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 μl of ab236453.

Lane 1 : U-2 OS whole cell lysate (Input).

Lane 2 : Control IgG in U-2 OS whole cell lysate.

Lane 3 : ab236453 in U-2 OS whole cell lysate.

For western blotting ab236453 was used at a 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-USP39 antibody - N-terminal (ab236453)

Predicted band size: 65 kDa

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Supplier Data

Western blot - Anti-USP39 antibody - N-terminal (AB236453)

Block : TBS-T 5% milk. Incubated overnight with primary antibody.

Treated with control or USP39 siRNA to show specificity.

All lanes:

Western blot - Anti-USP39 antibody - N-terminal (ab236453) at 1/2000 dilution

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell line) siCtrl at 40 µg

Lane 2:

U-2 OS (human bone osteosarcoma epithelial cell line) siUSP39 at 40 µg

Predicted band size: 65 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human USP39. The exact immunogen used to generate this antibody is proprietary information.

Q53GS9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 3 μl to ~400 μg of extact", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Purification notes

Crude serum containing sodium azide.

Storage buffer

Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP39 also known as U4/U6.U5 tri-snRNP-associated protein or Sad1 homologue functions as a deubiquitinating enzyme which is essential for the ubiquitin-proteasome system in cells. This protein has a molecular mass of approximately 65 kDa and is expressed abundantly in the nucleus. It participates in the regulation of pre-mRNA splicing by facilitating the assembly of the spliceosome an intricate molecular machine.

Biological function summary

USP39 plays a significant role in pre-mRNA splicing a process critical for the maturation of messenger RNA. It forms part of the U4/U6.U5 tri-snRNP complex which is essential for spliceosome assembly. This protein ensures proper spliceosome cycling by stabilizing snRNPs contributing to the accurate removal of introns from pre-mRNA sequences. Its involvement in RNA processing highlights its importance in gene expression regulation.

Pathways

USP39 integrates into the spliceosome pathway and is also linked to the mRNA surveillance pathway. These pathways are important for correct mRNA synthesis and degradation. USP39 functions alongside other splicing factors such as PRPF8 and EFTUD2 which are important components of the spliceosome machinery. By working synchronously with these proteins it maintains efficient RNA processing and cellular homeostasis.

USP39 has associations with various cancer types including breast and prostate cancer. Alterations in its expression or function can disrupt normal splicing and gene expression contributing to oncogenesis. In breast cancer for instance USP39 interacts with the BRCA1 protein influencing pathways linked to DNA repair and tumor suppression. In prostate cancer defects in its deubiquitination activity can lead to aberrant cell cycles and resistance to apoptosis highlighting its role in maintaining cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deubiquitinating enzyme that plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development (PubMed : 33127822, PubMed : 34614178). Plays a role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the precatalytic spliceosome (PubMed : 11350945, PubMed : 26912367). Specifically regulates immunoglobulin gene rearrangement in a spliceosome-dependent manner, which involves modulating chromatin interactions at the Igh locus and therefore plays an essential role in B-cell development (By similarity). Regulates AURKB mRNA levels, and thereby plays a role in cytokinesis and in the spindle checkpoint (PubMed : 18728397). Regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2 (PubMed : 30771428). Plays also an important role in DNA repair by controlling the recruitment of XRCC4/LIG4 to DNA double-strand breaks for non-homologous end-joining repair (PubMed : 34614178). Participates in antiviral activity by affecting the type I IFN signaling by stabilizing STAT1 and decreasing its 'Lys-6'-linked ubiquitination (PubMed : 33127822). Contributes to non-canonical Wnt signaling during epidermal differentiation (By similarity). Acts as a negative regulator NF-kappa-B activation through deubiquitination of 'Lys-48'-linked ubiquitination of NFKBIA (PubMed : 36651806).

See full target information USP39

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7032 PubMed37923718

2023

Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Donghai Cui,Zixiang Wang,Qianli Dang,Jing Wang,Junchao Qin,Jianping Song,Xiangyu Zhai,Yachao Zhou,Ling Zhao,Gang Lu,Hongbin Liu,Gang Liu,Runping Liu,Changshun Shao,Xiyu Zhang,Zhaojian Liu

Cell cycle (Georgetown, Tex.) 22:680-692 PubMed36305548

2022

miR-140-3p/usp36 axis mediates ubiquitination to regulate PKM2 and suppressed the malignant biological behavior of breast cancer through Warburg effect.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wu,Yile Jiao,Chen Zhou,Xinyi Guo,Zhenru Wu,Qing Lv

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-USP39 antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody - N-terminal (AB236453)

Flow Cytometry - Anti-USP39 antibody - N-terminal (AB236453)

Immunoprecipitation - Anti-USP39 antibody - N-terminal (AB236453)

Western blot - Anti-USP39 antibody - N-terminal (AB236453)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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