Anti-USP4 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody (AB236987)

U-251 MG (Human brain glioma cell line) cells stained for USP4 (green) using ab236987 at 1/166 dilution in ICC/IF, followed by Alexa Fluor^®488-conjugated goat anti-rabbit IgG (H+L). Counterstained with DAPI (blue).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubate with primary antibody at 4°C overnight.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP4 antibody (AB236987)

Paraffin-embedded human kidney tissue stained for USP4 using ab236987 at 1/500 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4° overnight. The primary is detected by a biotinylated secondary and visualized using an HRP conjugated SP system.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP4 antibody (AB236987)

Paraffin-embedded human colon cancer tissue stained for USP4 using ab236987 at 1/500 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4° overnight. The primary is detected by a biotinylated secondary and visualized using an HRP conjugated SP system.

• WB

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Western blot - Anti-USP4 antibody (AB236987)

All lanes:

Western blot - Anti-USP4 antibody (ab236987) at 1/500 dilution

All lanes:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 109 kDa

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