AB181105

Anti-USP4 antibody [EPR13846]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal USP4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

UNP, UNPH, USP4, Ubiquitin carboxyl-terminal hydrolase 4, Deubiquitinating enzyme 4, Ubiquitin thioesterase 4, Ubiquitin-specific-processing protease 4, Ubiquitous nuclear protein homolog

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody [EPR13846] (AB181105)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody [EPR13846] (AB181105)

Immunofluorescent staining of -20°C Acetone-fixed THP1 cells labeling USP4 using ab181105 at a 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at a 1/200 dilution and counter stained with DAPI.

Supplier Data

Western blot - Anti-USP4 antibody [EPR13846] (AB181105)

All lanes:

Western blot - Anti-USP4 antibody [EPR13846] (ab181105) at 1/1000 dilution

Lane 1:

HuT-78 cell lysate at 20 µg

Lane 2:

THP1 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 109 kDa

false

Carrier free

Anti-USP4 antibody [EPR13846] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13846

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP4 also known as Ubiquitin Specific Peptidase 4 is a deubiquitinating enzyme with an approximate mass of 108 kDa. It functions by removing ubiquitin from protein substrates impacting their stability and functionality. USP4 is expressed in various tissues including heart lung skeletal muscle liver and brain. Its enzymatic action is important for regulating protein turnover and preventing the degradation of proteins that require fine control.

Biological function summary

USP4 contributes to multiple cellular processes by maintaining protein homeostasis. It participates in regulation of the endoplasmic reticulum-associated degradation (ERAD) pathway and influences processes such as cellular transport and signal transduction. USP4 often functions within protein complexes that control these pathways including interactions with the spliceosome machinery which impact RNA processing and gene expression.

Pathways

USP4 plays a significant role in the TGF-beta signaling pathway and the WNT signaling pathway. It interacts with proteins such as SMAD2 SMAD3 and beta-catenin which are integral to these pathways for regulating transcriptional activity and cellular responses. Through the regulation of signaling components USP4 modulates key developmental and cellular growth processes.

USP4 has been linked to cancer and neurodegenerative diseases. Overexpression of USP4 is observed in certain cancers such as breast cancer where it affects the stability of proteins like TGF-beta receptor influencing tumor progression. In neurodegenerative diseases USP4's interaction with proteins like tau can contribute to the pathology as defects in protein clearance mechanisms play a role in disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins (PubMed : 16316627, PubMed : 16339847, PubMed : 16472766, PubMed : 20595234, PubMed : 22347420, PubMed : 25404403, PubMed : 28604766, PubMed : 30514904). Deubiquitinates PDPK1 (PubMed : 22347420). Deubiquitinates TRIM21 (PubMed : 16316627). Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface (PubMed : 16339847). Deubiquitinates HAS2 (PubMed : 28604766). Deubiquitinates MAVs leading to maintain MAVS protein stability, resulting in increased production of type I interferons (IFN-I) and enhanced antiviral innate immune responses against viral infections (PubMed : 39589880). Deubiquitinates RHEB in response to EGF signaling, promoting mTORC1 signaling (PubMed : 30514904). May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3 (PubMed : 20595234). This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP (PubMed : 20595234). May also play a role in the regulation of quality control in the ER (PubMed : 16339847).

See full target information USP4

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature aging 5:1750-1770 PubMed40903652

2025

The aging factor EPS8 induces disease-related protein aggregation through RAC signaling hyperactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Seda Koyuncu,Yaiza Dominguez-Canterla,Rafael Alis,Nassima Salarzai,Dunja Petrovic,Nuria Flames,David Vilchez

Scientific reports 15:7553 PubMed40038378

2025

USP37-stabilized SALL4 promotes the keloid formation by PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Fang,Zishuo Wang,Jianguo Xu,Miao Xu,Jiesong Zhou,Yuntong Zhang,Chunyu Xue

Cell cycle (Georgetown, Tex.) 22:986-1004 PubMed36762777

2023

Metformin attenuates multiple myeloma cell proliferation and encourages apoptosis by suppressing METTL3-mediated m6A methylation of THRAP3, RBM25, and USP4.

Applications

Unspecified application

Species

Unspecified reactive species

Cong-Jie Chen,Jie-Yun Huang,Jian-Qing Huang,Jia-Yi Deng,Xiao-Hui Shangguan,Ai-Zhen Chen,Long-Tian Chen,Wei-Hao Wu

Experimental cell research 396:112317 PubMed33038351

2020

Ubiquitin-specific protease 4 predicts an unfavorable prognosis and promotes malignant behaviors in vitro in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhi Wang,Li Zhou,Jun Lu,Bolun Jiang,Chengxi Liu,Zhiyong Liang,Weixun Zhou,Junchao Guo

Journal of cellular and molecular medicine 22:2944-2954 PubMed29542252

2018

Up-regulated deubiquitinase USP4 plays an oncogenic role in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weinan Guo,Jinyuan Ma,Tianli Pei,Tao Zhao,Sen Guo,Xiuli Yi,Yu Liu,Shiyu Wang,Guannan Zhu,Zhe Jian,Tianwen Gao,Chunying Li,Wenjun Liao,Qiong Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-USP4 antibody [EPR13846]

Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody [EPR13846] (AB181105)

Western blot - Anti-USP4 antibody [EPR13846] (AB181105)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise