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Rabbit Recombinant Monoclonal USP4 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody [EPR13846] - BSA and Azide free (AB250352), expandable thumbnail
  • Western blot - Anti-USP4 antibody [EPR13846] - BSA and Azide free (AB250352), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins (PubMed:16316627, PubMed:16472766, PubMed:16339847, PubMed:20595234, PubMed:22347420, PubMed:25404403). Deubiquitinates PDPK1 (PubMed:22347420). Deubiquitinates TRIM21 (PubMed:16316627). Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface (PubMed:16339847). May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3 (PubMed:20595234). This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP (PubMed:20595234). May also play a role in the regulation of quality control in the ER (PubMed:16339847).

Alternative names

Ubiquitin carboxyl-terminal hydrolase 4, Deubiquitinating enzyme 4, Ubiquitin thioesterase 4, Ubiquitin-specific-processing protease 4, Ubiquitous nuclear protein homolog, UNPH, UNP, USP4

Recommended products

Rabbit Recombinant Monoclonal USP4 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

Alternative names

Ubiquitin carboxyl-terminal hydrolase 4, Deubiquitinating enzyme 4, Ubiquitin thioesterase 4, Ubiquitin-specific-processing protease 4, Ubiquitous nuclear protein homolog, UNPH, UNP, USP4

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR13846

Purification technique

Affinity purification

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab250352 is the carrier-free version of Anti-USP4 antibody [EPR13846] ab181105.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

USP4 also known as Ubiquitin Specific Peptidase 4 is a deubiquitinating enzyme with an approximate mass of 108 kDa. It functions by removing ubiquitin from protein substrates impacting their stability and functionality. USP4 is expressed in various tissues including heart lung skeletal muscle liver and brain. Its enzymatic action is important for regulating protein turnover and preventing the degradation of proteins that require fine control.

Biological function summary

USP4 contributes to multiple cellular processes by maintaining protein homeostasis. It participates in regulation of the endoplasmic reticulum-associated degradation (ERAD) pathway and influences processes such as cellular transport and signal transduction. USP4 often functions within protein complexes that control these pathways including interactions with the spliceosome machinery which impact RNA processing and gene expression.

Pathways

USP4 plays a significant role in the TGF-beta signaling pathway and the WNT signaling pathway. It interacts with proteins such as SMAD2 SMAD3 and beta-catenin which are integral to these pathways for regulating transcriptional activity and cellular responses. Through the regulation of signaling components USP4 modulates key developmental and cellular growth processes.

Associated diseases and disorders

USP4 has been linked to cancer and neurodegenerative diseases. Overexpression of USP4 is observed in certain cancers such as breast cancer where it affects the stability of proteins like TGF-beta receptor influencing tumor progression. In neurodegenerative diseases USP4's interaction with proteins like tau can contribute to the pathology as defects in protein clearance mechanisms play a role in disease development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody [EPR13846] - BSA and Azide free (ab250352), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody [EPR13846] - BSA and Azide free (ab250352)

    This data was developed using Anti-USP4 antibody [EPR13846] ab181105, the same antibody clone in a different buffer formulation.Immunofluorescent staining of -20°C Acetone-fixed THP1 cells labeling USP4 using Anti-USP4 antibody [EPR13846] ab181105 at a 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at a 1/200 dilution and counter stained with DAPI.

  • Western blot - Anti-USP4 antibody [EPR13846] - BSA and Azide free (ab250352), expandable thumbnail

    Western blot - Anti-USP4 antibody [EPR13846] - BSA and Azide free (ab250352)

    This data was developed using Anti-USP4 antibody [EPR13846] ab181105, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-USP4 antibody [EPR13846] (Anti-USP4 antibody [EPR13846] ab181105) at 1/1000 dilution

    Lane 1: HuT-78 cell lysate at 20 µg

    Lane 2: THP1 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 109 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com