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AB250352

Anti-USP4 antibody [EPR13846] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal USP4 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

UNP, UNPH, USP4, Ubiquitin carboxyl-terminal hydrolase 4, Deubiquitinating enzyme 4, Ubiquitin thioesterase 4, Ubiquitin-specific-processing protease 4, Ubiquitous nuclear protein homolog

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody [EPR13846] - BSA and Azide free (AB250352)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody [EPR13846] - BSA and Azide free (AB250352)

This data was developed using ab181105, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of -20°C Acetone-fixed THP1 cells labeling USP4 using ab181105 at a 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at a 1/200 dilution and counter stained with DAPI.

Western blot - Anti-USP4 antibody [EPR13846] - BSA and Azide free (AB250352)
  • WB

Supplier Data

Western blot - Anti-USP4 antibody [EPR13846] - BSA and Azide free (AB250352)

This data was developed using ab181105, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-USP4 antibody [EPR13846] (<a href='/en-us/products/primary-antibodies/usp4-antibody-epr13846-ab181105'>ab181105</a>) at 1/1000 dilution

Lane 1:

HuT-78 cell lysate at 20 µg

Lane 2:

THP1 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 109 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13846

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250352 is the carrier-free version of ab181105.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP4 also known as Ubiquitin Specific Peptidase 4 is a deubiquitinating enzyme with an approximate mass of 108 kDa. It functions by removing ubiquitin from protein substrates impacting their stability and functionality. USP4 is expressed in various tissues including heart lung skeletal muscle liver and brain. Its enzymatic action is important for regulating protein turnover and preventing the degradation of proteins that require fine control.
Biological function summary

USP4 contributes to multiple cellular processes by maintaining protein homeostasis. It participates in regulation of the endoplasmic reticulum-associated degradation (ERAD) pathway and influences processes such as cellular transport and signal transduction. USP4 often functions within protein complexes that control these pathways including interactions with the spliceosome machinery which impact RNA processing and gene expression.

Pathways

USP4 plays a significant role in the TGF-beta signaling pathway and the WNT signaling pathway. It interacts with proteins such as SMAD2 SMAD3 and beta-catenin which are integral to these pathways for regulating transcriptional activity and cellular responses. Through the regulation of signaling components USP4 modulates key developmental and cellular growth processes.

USP4 has been linked to cancer and neurodegenerative diseases. Overexpression of USP4 is observed in certain cancers such as breast cancer where it affects the stability of proteins like TGF-beta receptor influencing tumor progression. In neurodegenerative diseases USP4's interaction with proteins like tau can contribute to the pathology as defects in protein clearance mechanisms play a role in disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins (PubMed : 16316627, PubMed : 16339847, PubMed : 16472766, PubMed : 20595234, PubMed : 22347420, PubMed : 25404403, PubMed : 28604766, PubMed : 30514904). Deubiquitinates PDPK1 (PubMed : 22347420). Deubiquitinates TRIM21 (PubMed : 16316627). Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface (PubMed : 16339847). Deubiquitinates HAS2 (PubMed : 28604766). Deubiquitinates RHEB in response to EGF signaling, promoting mTORC1 signaling (PubMed : 30514904). May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3 (PubMed : 20595234). This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP (PubMed : 20595234). May also play a role in the regulation of quality control in the ER (PubMed : 16339847).
See full target information USP4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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