JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB121234

Anti-USP40 antibody

4

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal USP40 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USP40 aa 750-900.

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase 40, Deubiquitinating enzyme 40, Ubiquitin thioesterase 40, Ubiquitin-specific-processing protease 40, USP40

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-USP40 antibody (AB121234)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-USP40 antibody (AB121234)

Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles and focal adhesions. Recommended concentration of ab121234 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP40 antibody (AB121234)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP40 antibody (AB121234)

ab121234, at 1/75 dilution, staining USP40 in Human tonsil tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Note : shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human USP40 aa 750-900. The exact immunogen used to generate this antibody is proprietary information.

Q9NVE5

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p>Use Buffer at pH 6 for antigen retrieval.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP40 also known as Ubiquitin Specific Peptidase 40 functions as a deubiquitinating enzyme. It belongs to a family of enzymes that remove ubiquitin from proteins. USP40 has a molecular mass of approximately 140 kDa. The expression of USP40 occurs mainly in tissues like the brain and testis. It lacks deubiquitinase activity on some substrates indicating specificity in its action compared to other ubiquitin proteases.
Biological function summary

The role of USP40 in cellular processes centers on regulating protein degradation. This regulation typically affects the stability and function of target proteins. It is not known to form a complex with other proteins but its action can still influence cellular homeostasis and stress responses indirectly. If not correctly regulated protein degradation can impact many cellular processes leading to varied biological outcomes.

Pathways

Several components of the ubiquitin-proteasome system involve USP40. This system plays an important role in maintaining protein quality control within cells. It interacts with proteins such as ubiquitin C-terminal hydrolase L1 (UCHL1) to modulate protein turnover and stability. Another pathway significantly influenced by USP40 is the autophagy-lysosome pathway where it impacts the recycling and degradation of cellular components.

Irregularities in USP40 have associations with neurodegenerative conditions like Parkinson's disease. In these contexts the dysfunction of protein degradation pathways contributes to the accumulation of misfolded proteins. USP40's association with Alzheimer's disease arises from its interaction with proteins involved in synaptic function and degradation processes like presenilin-1 (PSEN1). Enhancing the understanding of USP40 functions could provide insights into therapeutic strategies for these diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May be catalytically inactive.
See full target information USP40
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 19:13 PubMed38308285

2024

USP40 promotes hepatocellular carcinoma cell proliferation, migration and stemness by deubiquitinating and stabilizing Claudin1.

Applications

Unspecified application

Species

Unspecified reactive species

Qingsong Wu,Yuanyuan Qiu,Jinhui Guo,Zibo Yuan,Yingnan Yang,Qingwei Zhu,Zhe Zhang,Junwei Guo,Yanfang Wu,Junyu Zhang,Dongsheng Huang,Kangsheng Tu,Xiaoge Hu

Cancer letters 555:216025 PubMed36538983

2022

Miz1 promotes KRAS-driven lung tumorigenesis by repressing the protocadherin Pcdh10.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yang,Changchun Hou,Huashan Wang,Edith A Perez,Hanh Chi Do-Umehara,Huali Dong,Vinothini Arunagiri,Fangjia Tong,Michelle Van Scoyk,Minsu Cho,Xinyi Liu,Xiaodong Ge,Robert A Winn,Karen M Ridge,Xiaowei Wang,Navdeep S Chandel,Jing Liu
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB121943

Anti-WEE2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (AB121943)

  • 3
  • 1
Primary Antibodies

AB72115

Anti-WDR33 antibody

Immunoprecipitation - Anti-WDR33 antibody (AB72115)

  • 0
  • 0
Primary Antibodies

AB118471

Anti-ZNF238 antibody

Western blot - Anti-ZNF238 antibody (AB118471)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com