JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB244215

Anti-USP46 antibody [CL0364]

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Monoclonal USP46 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USP46 aa 100-250.

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase 46, Deubiquitinating enzyme 46, Ubiquitin thioesterase 46, Ubiquitin-specific-processing protease 46, USP46

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-USP46 antibody [CL0364] (AB244215)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-USP46 antibody [CL0364] (AB244215)

A-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for USP46 (green) using ab244215 at 4 μg/ml in ICC/IF. Microtubule and nuclear probes are visualized in red and blue respectively.

Western blot - Anti-USP46 antibody [CL0364] (AB244215)
  • WB

Supplier Data

Western blot - Anti-USP46 antibody [CL0364] (AB244215)

All lanes:

Western blot - Anti-USP46 antibody [CL0364] (ab244215) at 1 µg/mL

All lanes:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 42 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0364

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human USP46 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

P62068

Epitope

Binds to an epitope located within the peptidesequence TNETRCLNCE as determined by overlapping synthetic peptides.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP46 also known as Deubiquitinating enzyme 46 is an important player in the process of removing ubiquitin molecules from proteins. This enzyme belonging to the ubiquitin-specific protease family has a molecular mass of approximately 41 kDa. USP46 is widely expressed in various human tissues but significant levels are observed in the brain indicating its importance in neurological processes. Its deubiquitination activity modulate protein turn-over stability and signaling pathways influencing diverse cellular functions.
Biological function summary

USP46 modulates the ubiquitin-proteasome system by removing ubiquitin from substrate proteins which impacts their degradation or activity. It exists as part of a larger protein complex interacting with proteins such as WDR20 which enhance its activity. This interaction allows USP46 to regulate protein homeostasis within neurons and other cells. In the nervous system its activity supports synaptic function and plasticity contributing to effective neural communication and cognitive processes.

Pathways

USP46 mainly acts within the ubiquitin-proteasome pathway an essential mechanism for protein degradation and regulation. It interacts with proteins like UBE3A and UCH-L1 which are important for maintaining protein balance and cellular stress responses. Another important pathway involving USP46 is the neural plasticity and signaling pathway where it helps in regulating the degradation of synaptic proteins contributing to learning and memory.

USP46 has been linked to neurological disorders such as depression and schizophrenia. Abnormalities in its function or expression levels could disrupt normal synaptic activity and protein homeostasis. The enzyme shows a connection with proteins like GSK3β and DISC1 which are implicated in these disorders. Targeting USP46 activity holds potential in developing new therapeutic strategies for such neuro-psychiatric conditions offering avenues for further research and innovation in medical science.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. May act by mediating the deubiquitination of GAD1/GAD67 (By similarity). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed : 19075014, PubMed : 26388029). Not involved in deubiquitination of monoubiquitinated FANCD2 (PubMed : 19075014).
See full target information USP46
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e38070 PubMed39381248

2024

Neuroprotective effects of pink lotus oil in kainic acid-induced epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Ratchaniporn Kongsui,Teera Chanmanee,Tichanon Promsrisuk,Lars Klimaschewski,Napatr Sriraksa,Jinatta Jittiwat,Sitthisak Thongrong

Molecular medicine reports 23: PubMed33576437

2021

Downregulated expression levels of USP46 promote the resistance of ovarian cancer to cisplatin and are regulated by PUM2.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Xu,Bin Zhang,Wenlan Li
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB88795

Anti-USP46 antibody

Western blot - Anti-USP46 antibody (AB88795)

  • 3
  • 2

Complementary Products

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB179515

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (AB179515)

  • 3
  • 3
Primary Antibodies

AB180673

Anti-Caspase-11 antibody [EPR18628]

RabMAb|Recombinant

Western blot - Anti-Caspase-11 antibody [EPR18628] (AB180673)

  • 4
  • 2
Primary Antibodies

AB52489

Anti-MCM7/PRL antibody [EP1974Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [EP1974Y] (AB52489)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com