Rabbit Polyclonal USP47 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human USP47.
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Publications
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- International journal of molecular medicine 47:2021Long non‑coding RNA KCNQ1OT1 promotes nasopharyngeal carcinoma cell cisplatin resistance via the miR‑454/USP47 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Yuan,Zhiping Lou,Zhifeng Zhou,Xiaojun YanPubMed 33576460
- Frontiers in oncology 10:5654492021E2F7 Transcriptionally Inhibits MicroRNA-199b Expression to Promote USP47, Thereby Enhancing Colon Cancer Tumor Stem Cell Activity and Promoting the Occurrence of Colon Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiong Guo,Ling Liu,Qi Zhang,Weiming Yang,Yang ZhangPubMed 33489876
- Biotechnology and applied biochemistry 68:841-8482020USP47 promotes apoptosis in rat myocardial cells after ischemia/reperfusion injury via NF-κB activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Hu,Zhihui Ma,Zhong Chen,Bin ChenPubMed 32761659
- European journal of pharmacology 858:1724642019LINC00668 promotes tumorigenesis and progression through sponging miR-188-5p and regulating USP47 in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuai Yan et. al.PubMed 31233752
- Molecular biology of the cell 25:3851-602014Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyoko Sako-Kubota et. al.PubMed 25253721
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human USP47. The exact immunogen used to generate this antibody is proprietary information. Database link Q96K76
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.
Alternative names
Ubiquitin carboxyl-terminal hydrolase 47, Deubiquitinating enzyme 47, Ubiquitin thioesterase 47, Ubiquitin-specific-processing protease 47, USP47
Recommended products
Rabbit Polyclonal USP47 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human USP47.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human USP47. The exact immunogen used to generate this antibody is proprietary information. Database link Q96K76
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP47 also known as Ubiquitin Specific Peptidase 47 plays a role in deubiquitinating proteins by removing ubiquitin molecules from them. It is a member of the ubiquitin-specific proteases with a molecular mass of approximately 159 kDa. USP47 expresses widely in various tissues including the brain lungs and liver.
- Biological function summary
The function of USP47 focuses on maintaining levels of key proteins that require deubiquitination. It stabilizes beta-Catenin and other regulatory proteins by protecting them from proteasomal degradation. As a part of the larger ubiquitin-proteasome system USP47 works with other deubiquitinating enzymes but forms no stable complex with them. Its action ensures the regulation of protein homeostasis impacting cell cycle progression and DNA damage response.
- Pathways
USP47 interacts with the Wnt signaling pathway and the DNA damage repair pathway. By controlling the stability of beta-Catenin USP47 influences the Wnt signaling which plays a role in cell growth and differentiation. In the context of DNA repair USP47 interacts with other proteins like Flap endonuclease 1 (FEN1) to contribute to genome integrity by processing DNA lesions and maintaining genomic stability.
- Associated diseases and disorders
USP47 shows involvement with cancer and neurological disorders. Abnormal USP47 activity can lead to uncontrolled cell proliferation due to impacted beta-Catenin levels linking it to cancerous transformations. In neurological disorders USP47's effect on protein homeostasis may connect with proteins like Tau suggesting a role in neurodegenerative disorders where protein aggregation occurs.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-USP47 antibody (ab72143)
All lanes: Western blot - Anti-USP47 antibody (ab72143) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Predicted band size: 157 kDa
Observed band size: 170 kDa
Exposure time: 10s
Immunoprecipitation - Anti-USP47 antibody (ab72143)
Detection of USP47 by Western Blot of Immunprecipitate.
ab72143 at 0.1µg/ml for WB, using HeLa whole cell lysate immunoprecipitated using ab72143 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.All lanes: Immunoprecipitation - Anti-USP47 antibody (ab72143)
Predicted band size: 157 kDa
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