AB72143

Anti-USP47 antibody

Name: Anti-USP47 antibody (ab72143) | Abcam
Brand: Abcam Limited
SKU: AB72143
Price: 520 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal USP47 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human USP47.

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase 47, Deubiquitinating enzyme 47, Ubiquitin thioesterase 47, Ubiquitin-specific-processing protease 47, USP47

2 Images

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Immunoprecipitation - Anti-USP47 antibody (AB72143)

Detection of USP47 by Western Blot of Immunprecipitate.
ab72143 at 0.1µg/ml for WB, using HeLa whole cell lysate immunoprecipitated using ab72143 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-USP47 antibody (ab72143)

Predicted band size: 157 kDa

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Western blot - Anti-USP47 antibody (AB72143)

All lanes:

Western blot - Anti-USP47 antibody (ab72143) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 157 kDa

Observed band size: 170 kDa

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Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human USP47. The exact immunogen used to generate this antibody is proprietary information.

Q96K76

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP47 also known as Ubiquitin Specific Peptidase 47 plays a role in deubiquitinating proteins by removing ubiquitin molecules from them. It is a member of the ubiquitin-specific proteases with a molecular mass of approximately 159 kDa. USP47 expresses widely in various tissues including the brain lungs and liver.

Biological function summary

The function of USP47 focuses on maintaining levels of key proteins that require deubiquitination. It stabilizes beta-Catenin and other regulatory proteins by protecting them from proteasomal degradation. As a part of the larger ubiquitin-proteasome system USP47 works with other deubiquitinating enzymes but forms no stable complex with them. Its action ensures the regulation of protein homeostasis impacting cell cycle progression and DNA damage response.

Pathways

USP47 interacts with the Wnt signaling pathway and the DNA damage repair pathway. By controlling the stability of beta-Catenin USP47 influences the Wnt signaling which plays a role in cell growth and differentiation. In the context of DNA repair USP47 interacts with other proteins like Flap endonuclease 1 (FEN1) to contribute to genome integrity by processing DNA lesions and maintaining genomic stability.

USP47 shows involvement with cancer and neurological disorders. Abnormal USP47 activity can lead to uncontrolled cell proliferation due to impacted beta-Catenin levels linking it to cancerous transformations. In neurological disorders USP47's effect on protein homeostasis may connect with proteins like Tau suggesting a role in neurodegenerative disorders where protein aggregation occurs.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.

See full target information USP47

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:370 PubMed37564825

2023

Upregulation of ubiquitin carboxy‑terminal hydrolase 47 (USP47) in papillary thyroid carcinoma ex vivo and reduction of tumor cell malignant behaviors after USP47 knockdown by stabilizing SATB1 expression in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Guirong Luo,Liting Zhang,Wenyi Wu,Lihong Zhang,Jianqing Lin,Haihong Shi,Xinquan Wu,Yihuang Yu,Weigang Qiu,Jinyan Chen,Hansen Ding,Xinyao Chen

International journal of molecular medicine 47: PubMed33576460

2021

Long non‑coding RNA KCNQ1OT1 promotes nasopharyngeal carcinoma cell cisplatin resistance via the miR‑454/USP47 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yuan,Zhiping Lou,Zhifeng Zhou,Xiaojun Yan

Frontiers in oncology 10:565449 PubMed33489876

2021

E2F7 Transcriptionally Inhibits MicroRNA-199b Expression to Promote USP47, Thereby Enhancing Colon Cancer Tumor Stem Cell Activity and Promoting the Occurrence of Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong Guo,Ling Liu,Qi Zhang,Weiming Yang,Yang Zhang

Biotechnology and applied biochemistry 68:841-848 PubMed32761659

2020

USP47 promotes apoptosis in rat myocardial cells after ischemia/reperfusion injury via NF-κB activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Hu,Zhihui Ma,Zhong Chen,Bin Chen

European journal of pharmacology 858:172464 PubMed31233752

2019

LINC00668 promotes tumorigenesis and progression through sponging miR-188-5p and regulating USP47 in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Yan,Yinzi Yue,Jinbang Wang,Wenting Li,Mingming Sun,Chao Gu,Li Zeng

Molecular biology of the cell 25:3851-60 PubMed25253721

2014

Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoko Sako-Kubota,Nobutoshi Tanaka,Shigenori Nagae,Wenxiang Meng,Masatoshi Takeichi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-USP47 antibody

Immunoprecipitation - Anti-USP47 antibody (AB72143)

Western blot - Anti-USP47 antibody (AB72143)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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