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AB84531

Anti-USP47 antibody

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(1 Publication)

Rabbit Polyclonal USP47 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human USP47.

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase 47, Deubiquitinating enzyme 47, Ubiquitin thioesterase 47, Ubiquitin-specific-processing protease 47, USP47

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)

ab84531 at a 1 : 500 dilution, staining human USP47 in basal cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection : DAB staining using an Immunohistochemistry Accessory Kit.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)

ab84531 at a 1 : 500 dilution, staining human USP47 in prostate carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection : DAB staining using an Immunohistochemistry Accessory Kit

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)

ab84531 at a 1 : 100 dilution, staining human USP47 in a FFPE section of prostate adenocarcinoma, using Immunofluorescence. Detection : Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1 : 500.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP47 antibody (AB84531)

ab84531 at a 1 : 100 dilution, staining mouse USP47 in teratoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection : DAB staining using an Immunohistochemistry Accessory Kit.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human USP47. The exact immunogen used to generate this antibody is proprietary information.

Q96K76

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Alternative Products

Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Affinity purified using an epitope specific to USP47 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP47 also known as Ubiquitin Specific Peptidase 47 plays a role in deubiquitinating proteins by removing ubiquitin molecules from them. It is a member of the ubiquitin-specific proteases with a molecular mass of approximately 159 kDa. USP47 expresses widely in various tissues including the brain lungs and liver.
Biological function summary

The function of USP47 focuses on maintaining levels of key proteins that require deubiquitination. It stabilizes beta-Catenin and other regulatory proteins by protecting them from proteasomal degradation. As a part of the larger ubiquitin-proteasome system USP47 works with other deubiquitinating enzymes but forms no stable complex with them. Its action ensures the regulation of protein homeostasis impacting cell cycle progression and DNA damage response.

Pathways

USP47 interacts with the Wnt signaling pathway and the DNA damage repair pathway. By controlling the stability of beta-Catenin USP47 influences the Wnt signaling which plays a role in cell growth and differentiation. In the context of DNA repair USP47 interacts with other proteins like Flap endonuclease 1 (FEN1) to contribute to genome integrity by processing DNA lesions and maintaining genomic stability.

USP47 shows involvement with cancer and neurological disorders. Abnormal USP47 activity can lead to uncontrolled cell proliferation due to impacted beta-Catenin levels linking it to cancerous transformations. In neurological disorders USP47's effect on protein homeostasis may connect with proteins like Tau suggesting a role in neurodegenerative disorders where protein aggregation occurs.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.
See full target information USP47
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1026 PubMed39863586

2025

CHD6 has poly(ADP-ribose)- and DNA-binding domains and regulates PARP1/2-trapping inhibitor sensitivity via abasic site repair.

Applications

Unspecified application

Species

Unspecified reactive species

Luc Provencher,Wilson Nartey,Peter M Brownlee,Austin W Atkins,Jean-Philippe Gagné,Lou Baudrier,Nicholas S Y Ting,Cortt G Piett,Shujuan Fang,Dustin D Pearson,Shaun Moore,Pierre Billon,Zachary D Nagel,Guy G Poirier,Gareth J Williams,Aaron A Goodarzi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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