Rabbit Polyclonal USP48 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human USP48 aa 350-550.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human USP48 aa 350-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q86UV5
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Deubiquitinase that recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of polyubiquitin precursors as well as that of ubiquitinated proteins (PubMed:16214042, PubMed:34059922). Plays a role in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites. Plays an important role in cell cycle progression by deubiquitinating Aurora B/AURKB and thereby extending its stability (PubMed:34445214). In the context of H. pylori infection, stabilizes nuclear RELA through deubiquitination, thereby promoting the transcriptional activity of RELA to prolong TNFAIP3 de novo synthesis. Consequently, TNFAIP3 suppresses caspase activity and apoptotic cell death (PubMed:35913642). Functions also in the modulation of the ciliary and synaptic transport as well as cytoskeleton organization, which are key for photoreceptor function and homeostasis. To achieve this, stabilizes the levels of the retinal degeneration-associated proteins ARL3 and UNC119 using distinct mechanisms (PubMed:36293380). Plays a positive role in pyroptosis by stabilizing gasdermin E/GSDME through removal of its 'Lys-48'-linked ubiquitination (PubMed:36607699).
Alternative names
USP31, USP48, Ubiquitin carboxyl-terminal hydrolase 48, Deubiquitinating enzyme 48, Ubiquitin thioesterase 48, Ubiquitin-specific peptidase 48, Ubiquitin-specific protease 48, Ubiquitin-specific-processing protease 48
Recommended products
Rabbit Polyclonal USP48 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human USP48 aa 350-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human USP48 aa 350-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q86UV5
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity greater than 95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP48 also known as Ubiquitin Specific Peptidase 48 acts as a deubiquitinating enzyme. It specifically removes ubiquitin from target proteins influencing protein stability and function. USP48 has a molecular mass of approximately 130 kDa. This enzyme expresses in various tissues with significant levels observed in the brain and immune cells. Researchers recognize its involvement in protein homeostasis through regulation of protein degradation pathways.
- Biological function summary
Protein deubiquitination by USP48 affects cellular processes such as DNA repair transcription regulation and signal transduction. USP48 does not function as part of a stable complex but it associates transiently with substrates and other molecular partners during its activity. It has a role in maintaining genomic stability by modulating transcription factors and repair proteins through deubiquitination.
- Pathways
One can find USP48 participating in the NF-kB signaling pathway. It stabilizes RelA an important NF-kB subunit by removing ubiquitin which influences the transcription of genes involved in immune response and inflammation. USP48 also interacts with proteins such as OTUB1 in these pathways contributing to the balance of protein ubiquitination and deubiquitination within the cell.
- Associated diseases and disorders
Research connections exist between USP48 and several cancers including glioblastoma due to its regulation of pathways that control cell survival and proliferation. USP48's interactions with NF-kB impact glioblastoma progression by altering the expression of genes that promote cell growth. Defective USP48 activity also associates with neurodegenerative disorders where its modulation of protein stability could contribute to neuron degeneration through inadequate DNA repair mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-USP48 antibody (ab237765)
All lanes: Western blot - Anti-USP48 antibody (ab237765) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5: A549 (human lung carcinoma cell line) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 119 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP48 antibody (ab237765)
Paraffin-embedded human small intestine tissue stained for USP48 with ab237765 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP48 antibody (ab237765)
Paraffin-embedded human pancreatic cancer tissue stained for USP48 with ab237765 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunocytochemistry/ Immunofluorescence - Anti-USP48 antibody (ab237765)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for USP48 (green) using ab237765 at 1/66 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com