Rabbit Polyclonal USP48 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human USP48.
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- iScience 26:1064682023Latent CSN-CRL complexes are crucial for curcumin-induced apoptosis and recruited during adipogenesis to lipid droplets via small GTPase RAB18.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDawadschargal Dubiel,Jing Wang,Roland Hartig,Supattra Chaithongyot,Wolfgang Dubiel,Michael NaumannPubMed 37091236
- European journal of pain (London, England) 27:723-7342023USP48 alleviates bone cancer pain and regulates MrgC stabilization in spinal cord neurons of male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiacheng Yu,Bailing Hou,Yulin Huang,Xiaoyu Wang,Yue Qian,Ying Liang,Xiaoping Gu,Zhengliang Ma,Yue SunPubMed 36864656
- International journal of molecular sciences 23:2022The Deubiquitinating Enzyme USP48 Interacts with the Retinal Degeneration-Associated Proteins UNC119a and ARL3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Sánchez-Bellver,Andrea Férriz-Gordillo,Marc Carrillo-Pz,Laura Rabanal,Francesc R Garcia-Gonzalo,Gemma MarfanyPubMed 36293380
- International journal of molecular sciences 23:2022Ubiquitin Specific Protease USP48 Destabilizes NF-κB/p65 in Retinal Pigment Epithelium Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSerena Mirra,Laura Sánchez-Bellver,Carmela Casale,Alessandra Pescatore,Gemma MarfanyPubMed 36077078
- Cellular and molecular life sciences : CMLS 79:4612022USP48 and A20 synergistically promote cell survival in Helicobacter pylori infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhatcharida Jantaree,Supattra Chaithongyot,Olga Sokolova,Michael NaumannPubMed 35913642
- Journal of cell science 135:2022Imaging nanoscale nuclear structures with expansion microscopy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmma L Faulkner,Jeremy A Pike,Ruth M Densham,Evelyn Garlick,Steven G Thomas,Robert K Neely,Joanna R MorrisPubMed 35748225
- The journal of gene medicine 22:e31842020LINC00467 enhances head and neck squamous cell carcinoma progression and the epithelial-mesenchymal transition process via miR-299-5p/ubiquitin specific protease-48 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Chen,Yiying DingPubMed 32159247
- Molecular biology reports 46:1369-13752019Catalytic domain of deubiquitinylase USP48 directs interaction with Rel homology domain of nuclear factor kappaB transcription factor RelA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed Ghanem et. al.PubMed 30628021
- International journal of oncology 53:895-9032018Deubiquitinase USP48 promotes ATRA-induced granulocytic differentiation of acute promyelocytic leukemia cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLianlian Li,Yong Wang,Xiaoyu Zhang,Guanhua Song,Qiang Guo,Zhiyong Zhang,Yutao Diao,Haipeng Yin,Hongyan Liu,Guosheng JiangPubMed 29901102
- EMBO reports 18:1318-13302017Gli1-induced deubiquitinase USP48 aids glioblastoma tumorigenesis by stabilizing Gli1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAidong Zhou,Kangyu Lin,Sicong Zhang,Li Ma,Jianfei Xue,Saint-Aaron Morris,Kenneth D Aldape,Suyun HuangPubMed 28623188
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human USP48. The exact immunogen used to generate this antibody is proprietary information. Database link Q86UV5
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Predicted | Predicted | Expected |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Turkey | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Deubiquitinase that recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of polyubiquitin precursors as well as that of ubiquitinated proteins (PubMed:16214042, PubMed:34059922). Plays a role in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites. Plays an important role in cell cycle progression by deubiquitinating Aurora B/AURKB and thereby extending its stability (PubMed:34445214). In the context of H. pylori infection, stabilizes nuclear RELA through deubiquitination, thereby promoting the transcriptional activity of RELA to prolong TNFAIP3 de novo synthesis. Consequently, TNFAIP3 suppresses caspase activity and apoptotic cell death (PubMed:35913642). Functions also in the modulation of the ciliary and synaptic transport as well as cytoskeleton organization, which are key for photoreceptor function and homeostasis. To achieve this, stabilizes the levels of the retinal degeneration-associated proteins ARL3 and UNC119 using distinct mechanisms (PubMed:36293380). Plays a positive role in pyroptosis by stabilizing gasdermin E/GSDME through removal of its 'Lys-48'-linked ubiquitination (PubMed:36607699).
Alternative names
USP31, USP48, Ubiquitin carboxyl-terminal hydrolase 48, Deubiquitinating enzyme 48, Ubiquitin thioesterase 48, Ubiquitin-specific peptidase 48, Ubiquitin-specific protease 48, Ubiquitin-specific-processing protease 48
Recommended products
Rabbit Polyclonal USP48 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human USP48.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human USP48. The exact immunogen used to generate this antibody is proprietary information. Database link Q86UV5
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab72226 was affinity purified using an epitope specific to USP48 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP48 also known as Ubiquitin Specific Peptidase 48 acts as a deubiquitinating enzyme. It specifically removes ubiquitin from target proteins influencing protein stability and function. USP48 has a molecular mass of approximately 130 kDa. This enzyme expresses in various tissues with significant levels observed in the brain and immune cells. Researchers recognize its involvement in protein homeostasis through regulation of protein degradation pathways.
- Biological function summary
Protein deubiquitination by USP48 affects cellular processes such as DNA repair transcription regulation and signal transduction. USP48 does not function as part of a stable complex but it associates transiently with substrates and other molecular partners during its activity. It has a role in maintaining genomic stability by modulating transcription factors and repair proteins through deubiquitination.
- Pathways
One can find USP48 participating in the NF-kB signaling pathway. It stabilizes RelA an important NF-kB subunit by removing ubiquitin which influences the transcription of genes involved in immune response and inflammation. USP48 also interacts with proteins such as OTUB1 in these pathways contributing to the balance of protein ubiquitination and deubiquitination within the cell.
- Associated diseases and disorders
Research connections exist between USP48 and several cancers including glioblastoma due to its regulation of pathways that control cell survival and proliferation. USP48's interactions with NF-kB impact glioblastoma progression by altering the expression of genes that promote cell growth. Defective USP48 activity also associates with neurodegenerative disorders where its modulation of protein stability could contribute to neuron degeneration through inadequate DNA repair mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunoprecipitation - Anti-USP48 antibody (ab72226)
Detection of USP48 by Western Blot of Immunprecipitate.
ab72226 at 1µg/ml staining USP48 in HeLa whole cell lysate immunoprecipitated using ab72226 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second.All lanes: Immunoprecipitation - Anti-USP48 antibody (ab72226)
Predicted band size: 119 kDa
Western blot - Anti-USP48 antibody (ab72226)
All lanes: Western blot - Anti-USP48 antibody (ab72226) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Predicted band size: 119 kDa
Observed band size: 145 kDa
Exposure time: 3s
This image is courtesy of an anonymous customer reviewImmunocytochemistry - Anti-USP48 antibody (ab72226)
Immunocytochemistry analysis of paraformaldehyde-fixed human ARPE-19 cells permeabilized with 0.5% Triton X-100 for 20 minutes at RT, staining with ab72226 at 1/50 dilution. Secondary antibody was Alexa Fluor™ 488 goat anti-rabbit IgG at 1/300 dilution. Cells were incubated with the primary antibody for 12 hours at 4°C. Blocking was done with 3% BSA for 1 hour at 25°C.
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