AB72226

Anti-USP48 antibody

Name: Anti-USP48 antibody. Rabbit polyclonal (ab72226) | Abcam
Brand: Abcam Limited
SKU: AB72226
Price: 520 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(10 Publications)

Rabbit Polyclonal USP48 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human USP48.

View Alternative Names

USP31, USP48, Ubiquitin carboxyl-terminal hydrolase 48, Deubiquitinating enzyme 48, Ubiquitin thioesterase 48, Ubiquitin-specific peptidase 48, Ubiquitin-specific protease 48, Ubiquitin-specific-processing protease 48

3 Images

AbReview81927****

Immunocytochemistry - Anti-USP48 antibody (AB72226)

Immunocytochemistry analysis of paraformaldehyde-fixed human ARPE-19 cells permeabilized with 0.5% Triton X-100 for 20 minutes at RT, staining with ab72226 at 1/50 dilution. Secondary antibody was Alexa Fluor™ 488 goat anti-rabbit IgG at 1/300 dilution. Cells were incubated with the primary antibody for 12 hours at 4°C. Blocking was done with 3% BSA for 1 hour at 25°C.

This image is courtesy of an anonymous Abreview

Unknown

Immunoprecipitation - Anti-USP48 antibody (AB72226)

Detection of USP48 by Western Blot of Immunprecipitate.
ab72226 at 1µg/ml staining USP48 in HeLa whole cell lysate immunoprecipitated using ab72226 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-USP48 antibody (ab72226)

Predicted band size: 119 kDa

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Unknown

Western blot - Anti-USP48 antibody (AB72226)

All lanes:

Western blot - Anti-USP48 antibody (ab72226) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 119 kDa

Observed band size: 145 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IP

applications

Immunogen

Synthetic Peptide within Human USP48. The exact immunogen used to generate this antibody is proprietary information.

Q86UV5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab72226 was affinity purified using an epitope specific to USP48 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

USP48 also known as Ubiquitin Specific Peptidase 48 acts as a deubiquitinating enzyme. It specifically removes ubiquitin from target proteins influencing protein stability and function. USP48 has a molecular mass of approximately 130 kDa. This enzyme expresses in various tissues with significant levels observed in the brain and immune cells. Researchers recognize its involvement in protein homeostasis through regulation of protein degradation pathways.

Biological function summary

Protein deubiquitination by USP48 affects cellular processes such as DNA repair transcription regulation and signal transduction. USP48 does not function as part of a stable complex but it associates transiently with substrates and other molecular partners during its activity. It has a role in maintaining genomic stability by modulating transcription factors and repair proteins through deubiquitination.

Pathways

One can find USP48 participating in the NF-kB signaling pathway. It stabilizes RelA an important NF-kB subunit by removing ubiquitin which influences the transcription of genes involved in immune response and inflammation. USP48 also interacts with proteins such as OTUB1 in these pathways contributing to the balance of protein ubiquitination and deubiquitination within the cell.

Research connections exist between USP48 and several cancers including glioblastoma due to its regulation of pathways that control cell survival and proliferation. USP48's interactions with NF-kB impact glioblastoma progression by altering the expression of genes that promote cell growth. Defective USP48 activity also associates with neurodegenerative disorders where its modulation of protein stability could contribute to neuron degeneration through inadequate DNA repair mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Deubiquitinase that recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of polyubiquitin precursors as well as that of ubiquitinated proteins (PubMed : 16214042, PubMed : 34059922). Plays a role in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites. Plays an important role in cell cycle progression by deubiquitinating Aurora B/AURKB and thereby extending its stability (PubMed : 34445214). In the context of H. pylori infection, stabilizes nuclear RELA through deubiquitination, thereby promoting the transcriptional activity of RELA to prolong TNFAIP3 de novo synthesis. Consequently, TNFAIP3 suppresses caspase activity and apoptotic cell death (PubMed : 35913642). Functions also in the modulation of the ciliary and synaptic transport as well as cytoskeleton organization, which are key for photoreceptor function and homeostasis. To achieve this, stabilizes the levels of the retinal degeneration-associated proteins ARL3 and UNC119 using distinct mechanisms (PubMed : 36293380). Plays a positive role in pyroptosis by stabilizing gasdermin E/GSDME through removal of its 'Lys-48'-linked ubiquitination (PubMed : 36607699).

See full target information USP48

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106468 PubMed37091236

2023

Latent CSN-CRL complexes are crucial for curcumin-induced apoptosis and recruited during adipogenesis to lipid droplets via small GTPase RAB18.

Applications

Unspecified application

Species

Unspecified reactive species

Dawadschargal Dubiel,Jing Wang,Roland Hartig,Supattra Chaithongyot,Wolfgang Dubiel,Michael Naumann

European journal of pain (London, England) 27:723-734 PubMed36864656

2023

USP48 alleviates bone cancer pain and regulates MrgC stabilization in spinal cord neurons of male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jiacheng Yu,Bailing Hou,Yulin Huang,Xiaoyu Wang,Yue Qian,Ying Liang,Xiaoping Gu,Zhengliang Ma,Yue Sun

International journal of molecular sciences 23: PubMed36293380

2022

The Deubiquitinating Enzyme USP48 Interacts with the Retinal Degeneration-Associated Proteins UNC119a and ARL3.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Sánchez-Bellver,Andrea Férriz-Gordillo,Marc Carrillo-Pz,Laura Rabanal,Francesc R Garcia-Gonzalo,Gemma Marfany

International journal of molecular sciences 23: PubMed36077078

2022

Ubiquitin Specific Protease USP48 Destabilizes NF-κB/p65 in Retinal Pigment Epithelium Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Serena Mirra,Laura Sánchez-Bellver,Carmela Casale,Alessandra Pescatore,Gemma Marfany

Cellular and molecular life sciences : CMLS 79:461 PubMed35913642

2022

USP48 and A20 synergistically promote cell survival in Helicobacter pylori infection.

Applications

Unspecified application

Species

Unspecified reactive species

Phatcharida Jantaree,Supattra Chaithongyot,Olga Sokolova,Michael Naumann

Journal of cell science 135: PubMed35748225

2022

Imaging nanoscale nuclear structures with expansion microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Emma L Faulkner,Jeremy A Pike,Ruth M Densham,Evelyn Garlick,Steven G Thomas,Robert K Neely,Joanna R Morris

The journal of gene medicine 22:e3184 PubMed32159247

2020

LINC00467 enhances head and neck squamous cell carcinoma progression and the epithelial-mesenchymal transition process via miR-299-5p/ubiquitin specific protease-48 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Chen,Yiying Ding

Molecular biology reports 46:1369-1375 PubMed30628021

2019

Catalytic domain of deubiquitinylase USP48 directs interaction with Rel homology domain of nuclear factor kappaB transcription factor RelA.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Ghanem,Katrin Schweitzer,Michael Naumann

International journal of oncology 53:895-903 PubMed29901102

2018

Deubiquitinase USP48 promotes ATRA-induced granulocytic differentiation of acute promyelocytic leukemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lianlian Li,Yong Wang,Xiaoyu Zhang,Guanhua Song,Qiang Guo,Zhiyong Zhang,Yutao Diao,Haipeng Yin,Hongyan Liu,Guosheng Jiang

EMBO reports 18:1318-1330 PubMed28623188

2017

Gli1-induced deubiquitinase USP48 aids glioblastoma tumorigenesis by stabilizing Gli1.

Applications

Unspecified application

Species

Unspecified reactive species

Aidong Zhou,Kangyu Lin,Sicong Zhang,Li Ma,Jianfei Xue,Saint-Aaron Morris,Kenneth D Aldape,Suyun Huang

View all publications

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Product promise

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Anti-USP48 antibody

Immunocytochemistry - Anti-USP48 antibody (AB72226)

Immunoprecipitation - Anti-USP48 antibody (AB72226)

Western blot - Anti-USP48 antibody (AB72226)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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