Anti-USP5 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-USP5 antibody (AB241311)

USP5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241311 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab241311 at 1 μg/ml.

Lane 1 : ab241311 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-USP5 antibody (ab241311)

Predicted band size: 96 kDa

false

• WB

Supplier Data

Western blot - Anti-USP5 antibody (AB241311)

All lanes:

Western blot - Anti-USP5 antibody (ab241311) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 96 kDa

true

Exposure time: 30s