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AB154170

Anti-USP5 antibody [EPR10454]

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(12 Publications)

Rabbit Recombinant Monoclonal USP5 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

View Alternative Names

ISOT, USP5, Ubiquitin carboxyl-terminal hydrolase 5, Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5

6 Images
Flow Cytometry (Intracellular) - Anti-USP5 antibody [EPR10454] (AB154170)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-USP5 antibody [EPR10454] (AB154170)

Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling USP5with purified ab154170 at 1/50 dilution (10μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-USP5 antibody [EPR10454] (AB154170)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-USP5 antibody [EPR10454] (AB154170)

Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling USP5 with purified ab154170 at 1/50 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunocytochemistry/ Immunofluorescence - Anti-USP5 antibody [EPR10454] (AB154170)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-USP5 antibody [EPR10454] (AB154170)

Immunofluorescent analysis of MCF7 cells labeling USP5 with unpurified ab154170 at 1/50 dilution.

Western blot - Anti-USP5 antibody [EPR10454] (AB154170)
  • WB

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Western blot - Anti-USP5 antibody [EPR10454] (AB154170)

All lanes:

Western blot - Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 96 kDa

false

Western blot - Anti-USP5 antibody [EPR10454] (AB154170)
  • WB

Unknown

Western blot - Anti-USP5 antibody [EPR10454] (AB154170)

All lanes:

Western blot - Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution

Lane 1:

Mouse brain lysates at 10 µg

Lane 2:

Rat brain lysates at 10 µg

Lane 3:

Rat heart lysates at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 96 kDa

false

Western blot - Anti-USP5 antibody [EPR10454] (AB154170)
  • WB

Unknown

Western blot - Anti-USP5 antibody [EPR10454] (AB154170)

All lanes:

Western blot - Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Human brain lysate at 20 µg

Lane 4:

Mouse brain lysate at 20 µg

Lane 5:

Rat brain lysate at 20 µg

Lane 6:

Mouse heart lysate at 20 µg

Lane 7:

Rat heart lysate at 20 µg

Lane 8:

Mouse kidney lysate at 20 µg

Lane 9:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 96 kDa

Observed band size: 96 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10454

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/50 - 1/100.</strong></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000 - 1/10000.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p>For unpurified use at 1/10 - 1/100. <a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000 - 1/10000.</strong></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000 - 1/10000.</strong></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target USP5 also known as ubiquitin-specific protease 5 or isopeptidase T functions as a deubiquitinating enzyme. This protein has a mass of about 169 kDa and plays a critical role in removing ubiquitin moieties from ubiquitin-conjugated protein substrates. USP5 achieves this by cleaving at the ubiquitin C-terminal glycine which releases free ubiquitin and recycles it for future use. Expression of USP5 occurs broadly throughout different tissues indicating its general importance in cellular processes.
Biological function summary

This enzyme helps maintain ubiquitin homeostasis by disassembling unanchored polyubiquitin chains. USP5 is not known to be part of a larger complex but it interacts with components of the ubiquitin-proteasome system contributing to protein quality control. By processing unanchored ubiquitin chains USP5 ensures a sufficient supply of ubiquitin preventing potential cellular stress due to ubiquitin depletion.

Pathways

USP5 integrates into the ubiquitin-proteasome pathway and proteostasis network. It collaborates with other deubiquitinating enzymes like USP14 and UCHL5 modulating the degradation of polyubiquitinated proteins. These pathways play essential roles in regulating protein turnover and disruptions in these processes can impact cell cycle and apoptosis highlighting the importance of USP5 in maintaining cellular homeostasis.

Experimental findings suggest USP5 involvement in several cancers and neurodegenerative diseases such as Alzheimer's disease. In cancer altered USP5 activity can disrupt the balance between protein synthesis and degradation promoting tumor progression. In Alzheimer's its role in ubiquitin homeostasis implicates it in the management of protein aggregates that characterize the disorder. USP5's relationship with other proteins like p53 in cancer and tau in Alzheimer's provides critical insights into its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
See full target information USP5
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Inflammation 48:3446-3457 PubMed40053057

2025

USP5 Deletion Inhibits KD Serum Induced-Human Coronary Artery Endothelial Cell Dysfunction by Regulating the NFATC1/TLR4-Mediated NF-κB Signaling Pathway in Kawasaki Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lidan Yao,Yupeng Lai,Heng Li,Sihan Chen,Xianjia Yu,Ni Zhou,Dandan Lang

Cell death & disease 14:525 PubMed37582769

2023

Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Yang,Yuxi Ou,Quan Zhou,Yingchun Liang,Weijian Li,Yiling Chen,Wensun Chen,Siqi Wu,Yifan Chen,Xiyu Dai,Xinan Chen,Tian Chen,Shengming Jin,Yufei Liu,Limin Zhang,Shenghua Liu,Yun Hu,Lujia Zou,Shanhua Mao,Haowen Jiang

BMC cancer 23:28 PubMed36611139

2023

EOAI, a ubiquitin-specific peptidase 5 inhibitor, prevents non-small cell lung cancer progression by inducing DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Zheng,Longhao Wang,Xiaoyu Niu,Yongjun Guo,Jiuzhou Zhao,Lifeng Li,Jie Zhao

Frontiers in pharmacology 13:858901 PubMed35600879

2022

ARV-771 Acts as an Inducer of Cell Cycle Arrest and Apoptosis to Suppress Hepatocellular Carcinoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanfei Deng,Cuifu Yu,Lushi Chen,Xin Zhang,Qiucheng Lei,Qing Liu,Gengxi Cai,Fang Liu

Cell death & disease 13:312 PubMed35387981

2022

SIX5-activated LINC01468 promotes lung adenocarcinoma progression by recruiting SERBP1 to regulate SERPINE1 mRNA stability and recruiting USP5 to facilitate PAI1 protein deubiquitylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yuan,Danyang Zhou,Feifei Chen,Zhenhua Yang,Wei Gu,Kai Zhang

Autophagy 18:2615-2635 PubMed35253629

2022

Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Xiao,Jianqun Wang,Xiaojing Wang,Shuang Cai,Yanhua Guo,Lin Ye,Dan Li,Anpei Hu,Shikai Jin,Boling Yuan,Yi Zhou,Qilan Li,Qiangsong Tong,Liduan Zheng

Frontiers in pharmacology 12:720307 PubMed34483932

2021

USP5 Sustains the Proliferation of Glioblastoma Through Stabilization of CyclinD1.

Applications

Unspecified application

Species

Unspecified reactive species

Gen Li,Tianquan Yang,Yanling Chen,Jianping Bao,Di Wu,Xiaohan Hu,Chenxi Feng,Lixiao Xu,Mei Li,Gang Li,Meifang Jin,Yunyun Xu,Rui Zhang,Guanghui Qian,Jian Pan

Molecular cell 80:633-647.e7 PubMed33217317

2020

Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases mA Methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Lung Sun,Allen C Zhu,Yawei Gao,Hideki Terajima,Qili Fei,Shun Liu,Linda Zhang,Zijie Zhang,Bryan T Harada,Yu-Ying He,Marc B Bissonnette,Mien-Chie Hung,Chuan He

Cell death & disease 11:863 PubMed33060560

2020

Mesenchymal stem cells reverse EMT process through blocking the activation of NF-κB and Hedgehog pathways in LPS-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Xiao,Wanxue He,Wei Guan,Fei Hou,Peng Yan,Jianqiao Xu,Ting Zhou,Yuhong Liu,Lixin Xie

Journal of cellular biochemistry 121:642-650 PubMed31452281

2019

miR-125a suppresses malignancy of multiple myeloma by reducing the deubiquitinase USP5.

Applications

Unspecified application

Species

Unspecified reactive species

Liting Wu,Cui Zhang,Min Chu,Yingchao Fan,Lu Wei,Zhumeng Li,Yonghua Yao,Wenfang Zhuang
View all publications
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