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Rabbit Recombinant Monoclonal USP5 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

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Images

Western blot - Anti-USP5 antibody [EPR10454] (AB154170), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-USP5 antibody [EPR10454] (AB154170), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-USP5 antibody [EPR10454] (AB154170), expandable thumbnail
  • Western blot - Anti-USP5 antibody [EPR10454] (AB154170), expandable thumbnail
  • Western blot - Anti-USP5 antibody [EPR10454] (AB154170), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/50

Notes

For unpurified use at 1/50 - 1/100.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

For unpurified use at 1/1000 - 1/10000.

Species

Human

Dilution info

1/1000

Notes

For unpurified use at 1/1000 - 1/10000.

Species

Rat

Dilution info

1/1000

Notes

For unpurified use at 1/1000 - 1/10000.

Tested
Tested

Species

Human

Dilution info

1/50

Notes

For unpurified use at 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Alternative names

Ubiquitin carboxyl-terminal hydrolase 5, Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5, ISOT, USP5

Recommended products

Rabbit Recombinant Monoclonal USP5 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR10454

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The target USP5 also known as ubiquitin-specific protease 5 or isopeptidase T functions as a deubiquitinating enzyme. This protein has a mass of about 169 kDa and plays a critical role in removing ubiquitin moieties from ubiquitin-conjugated protein substrates. USP5 achieves this by cleaving at the ubiquitin C-terminal glycine which releases free ubiquitin and recycles it for future use. Expression of USP5 occurs broadly throughout different tissues indicating its general importance in cellular processes.

Biological function summary

This enzyme helps maintain ubiquitin homeostasis by disassembling unanchored polyubiquitin chains. USP5 is not known to be part of a larger complex but it interacts with components of the ubiquitin-proteasome system contributing to protein quality control. By processing unanchored ubiquitin chains USP5 ensures a sufficient supply of ubiquitin preventing potential cellular stress due to ubiquitin depletion.

Pathways

USP5 integrates into the ubiquitin-proteasome pathway and proteostasis network. It collaborates with other deubiquitinating enzymes like USP14 and UCHL5 modulating the degradation of polyubiquitinated proteins. These pathways play essential roles in regulating protein turnover and disruptions in these processes can impact cell cycle and apoptosis highlighting the importance of USP5 in maintaining cellular homeostasis.

Associated diseases and disorders

Experimental findings suggest USP5 involvement in several cancers and neurodegenerative diseases such as Alzheimer's disease. In cancer altered USP5 activity can disrupt the balance between protein synthesis and degradation promoting tumor progression. In Alzheimer's its role in ubiquitin homeostasis implicates it in the management of protein aggregates that characterize the disorder. USP5's relationship with other proteins like p53 in cancer and tau in Alzheimer's provides critical insights into its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-USP5 antibody [EPR10454] (ab154170), expandable thumbnail

    Western blot - Anti-USP5 antibody [EPR10454] (ab154170)

    All lanes: Western blot - Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Human brain lysate at 20 µg

    Lane 4: Mouse brain lysate at 20 µg

    Lane 5: Rat brain lysate at 20 µg

    Lane 6: Mouse heart lysate at 20 µg

    Lane 7: Rat heart lysate at 20 µg

    Lane 8: Mouse kidney lysate at 20 µg

    Lane 9: Rat kidney lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 96 kDa

    Observed band size: 96 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-USP5 antibody [EPR10454] (ab154170), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-USP5 antibody [EPR10454] (ab154170)

    Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling USP5 with purified ab154170 at 1/50 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-USP5 antibody [EPR10454] (ab154170), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-USP5 antibody [EPR10454] (ab154170)

    Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling USP5with purified ab154170 at 1/50 dilution (10μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-USP5 antibody [EPR10454] (ab154170), expandable thumbnail

    Western blot - Anti-USP5 antibody [EPR10454] (ab154170)

    All lanes: Western blot - Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution

    Lane 1: MCF7 cell lysate at 10 µg

    Lane 2: A549 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 96 kDa

  • Western blot - Anti-USP5 antibody [EPR10454] (ab154170), expandable thumbnail

    Western blot - Anti-USP5 antibody [EPR10454] (ab154170)

    All lanes: Western blot - Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution

    Lane 1: Mouse brain lysates at 10 µg

    Lane 2: Rat brain lysates at 10 µg

    Lane 3: Rat heart lysates at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 96 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-USP5 antibody [EPR10454] (ab154170), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-USP5 antibody [EPR10454] (ab154170)

    Immunofluorescent analysis of MCF7 cells labeling USP5 with unpurified ab154170 at 1/50 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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