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AB224725

Anti-USP6 antibody

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(2 Publications)

Rabbit Polyclonal USP6 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human USP6 aa 1100 to C-terminus.

View Alternative Names

HRP1, TRE2, USP6, Ubiquitin carboxyl-terminal hydrolase 6, Deubiquitinating enzyme 6, Proto-oncogene TRE-2, RN-tre, Ubiquitin thioesterase 6, Ubiquitin-specific-processing protease 6

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-USP6 antibody (AB224725)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-USP6 antibody (AB224725)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for USP6 (green) using ab224725 at 1/100 dilution in ICC/IF. Secondary : Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP6 antibody (AB224725)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP6 antibody (AB224725)

Paraffin embedded human ovarian cancer tissue stained for USP6 with ab224725 (1/100 dilution) in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human USP6 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P35125

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The USP6 protein also known as Tre-2 or Ubiquitin-Specific Peptidase 6 serves as a deubiquitinating enzyme. It has a molecular mass of approximately 140 kDa. USP6 catalyzes the removal of ubiquitin from specific substrates impacting their stability and degradation. You can find USP6 expression in a variety of tissues but it primarily shows in skeletal muscle and brain tissues.
Biological function summary

USP6 regulates numerous cellular processes by modifying protein ubiquitination levels. This protein often interacts with components of the endosomal sorting complex required for transport (ESCRT) playing a part in the regulation of membrane trafficking. Additionally USP6 influences cell signaling pathways by modulating the turnover of receptor proteins important for cell communication.

Pathways

Several cellular networks involve USP6 that control protein degradation and signal transduction. In particular it plays a role in the Wnt signaling pathway impacting cell proliferation and differentiation. The protein also associates with β-catenin through this pathway affecting cellular processes such as transcriptional activation and cell cycle regulation.

Aberrant USP6 expression links with various conditions. It has a connection to aneurysmal bone cysts where its upregulation results in tumor formation due to enhanced osteoclast activity. The USP6 gene fusion with another protein CDH11 is implicated in this disorder altering normal cellular signaling. Furthermore USP6's dysregulation can also relate to certain soft tissue tumors indicating its role in tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation. May act as a GTPase-activating protein for RAB3A (PubMed : 19077034).
See full target information USP6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed41034039

2025

Expressions of VEGF, Vasohibin-1, and Vasohibin-2 in Benign Parotid Gland Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Yutaka Tateda,Takahiro Suzuki,Teruyuki Sato,Kazue Ise,Hiroki Shimada,Yasuhiro Nakamura,Nobuo Ohta

Translational cancer research 13:2691-2703 PubMed38988918

2024

Increase in GPIHBP1 expression in advanced stage colorectal cancer indicates poor immune surveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Min Gao,Liting Liao,Zhijie Lin,Xiangyu Hu,Linhui Jia,Weijuan Gong,Xiaoqin Jia
View all publications
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Product promise

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