Rabbit Polyclonal USP6 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human USP6 aa 1100-1400.
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Publications
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- BMC cancer 23:10422023Low-dose adropin stimulates inflammasome activation of macrophage via mitochondrial ROS involved in colorectal cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinghui Jia,Liting Liao,Yongshuai Jiang,Xiangyu Hu,Guotao Lu,Weiming Xiao,Weijuan Gong,Xiaoqin JiaPubMed 37904094
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human USP6 aa 1100-1400. The exact immunogen used to generate this antibody is proprietary information. Database link P35125
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation. May act as a GTPase-activating protein for RAB3A (PubMed:19077034).
Alternative names
HRP1, TRE2, USP6, Ubiquitin carboxyl-terminal hydrolase 6, Deubiquitinating enzyme 6, Proto-oncogene TRE-2, RN-tre, Ubiquitin thioesterase 6, Ubiquitin-specific-processing protease 6
Recommended products
Rabbit Polyclonal USP6 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human USP6 aa 1100-1400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human USP6 aa 1100-1400. The exact immunogen used to generate this antibody is proprietary information. Database link P35125
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The USP6 protein also known as Tre-2 or Ubiquitin-Specific Peptidase 6 serves as a deubiquitinating enzyme. It has a molecular mass of approximately 140 kDa. USP6 catalyzes the removal of ubiquitin from specific substrates impacting their stability and degradation. You can find USP6 expression in a variety of tissues but it primarily shows in skeletal muscle and brain tissues.
- Biological function summary
USP6 regulates numerous cellular processes by modifying protein ubiquitination levels. This protein often interacts with components of the endosomal sorting complex required for transport (ESCRT) playing a part in the regulation of membrane trafficking. Additionally USP6 influences cell signaling pathways by modulating the turnover of receptor proteins important for cell communication.
- Pathways
Several cellular networks involve USP6 that control protein degradation and signal transduction. In particular it plays a role in the Wnt signaling pathway impacting cell proliferation and differentiation. The protein also associates with β-catenin through this pathway affecting cellular processes such as transcriptional activation and cell cycle regulation.
- Associated diseases and disorders
Aberrant USP6 expression links with various conditions. It has a connection to aneurysmal bone cysts where its upregulation results in tumor formation due to enhanced osteoclast activity. The USP6 gene fusion with another protein CDH11 is implicated in this disorder altering normal cellular signaling. Furthermore USP6's dysregulation can also relate to certain soft tissue tumors indicating its role in tumorigenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-USP6 antibody (ab224725)
All lanes: Western blot - Anti-USP6 antibody (ab224725) at 1/500 dilution
All lanes: rat kidney tissue lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 159 kDa
Observed band size: 159 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP6 antibody (ab224725)
Paraffin embedded human ovarian cancer tissue stained for USP6 with ab224725 (1/100 dilution) in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-USP6 antibody (ab224725)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for USP6 (green) using ab224725 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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