AB213203

Anti-Uteroglobin antibody [EPR19846]

RabMAb
Recombinant
What is this?

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(15 Publications)

Anti-Uteroglobin antibody [EPR19846] (ab213203) is a rabbit monoclonal antibody detecting Uteroglobin in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Cc10, Ugb, Utg, Scgb1a1, Uteroglobin, Club cell 17 kDa protein, Club cell phospholipid-binding protein, Club cells 10 kDa secretory protein, PCB-binding protein, Secretoglobin family 1A member 1, CCPBP, CC10

7 Images

Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat lung tissue labeling Uteroglobin with ab213203 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Positive staining on the airway epithelium of rat lung [PMID : 22916196].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Uteroglobin with ab213203 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Strong cytoplasmic staining on the airway epithelium of mouse lung. However, there is some non-specific cytoplasmic staining on the alveolar cells of mouse lung. The correct staining on the airway epithelium of mouse lung is observed when using immunofluorescence detection method.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Uteroglobin with ab213203 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Positive staining on the airway epithelium of mouse lung [PMID : 22916196].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, paraffin-embedded mouse lung tissue labeling Uteroglobin antibody with ab213203 at 1/1000 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution.

Positive staining of Uteroglobin on the airway epithelium of mouse lung is observed.

The nuclear counterstain is DAPI (blue).

Heat mediated antigen retrieval using citrate buffer pH 6.0

The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Uteroglobin with ab213203 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Strong cytoplasmic staining on the airway epithelium of rat lung is observed [PMID : 22916196].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Uteroglobin was immunoprecipitated from 0.35 mg of mouse lung lysate with ab213203 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab213203 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : Mouse lung lysate 10ug (Input).

Lane 2 : ab213203 IP in mouse lung lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab213203 in mouse lung lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] (ab213203)

Predicted band size: 10 kDa

false

Supplier Data

Western blot - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Uteroglobin antibody [EPR19846] (ab213203) at 1/1000 dilution

Lane 1:

Mouse lung lysate at 20 µg

Lane 2:

Mouse heart lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

false

Exposure time: 3s

Carrier free

Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free
Biotin

Biotin Anti-Uteroglobin antibody [EPR19846]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19846

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-Fr, IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "We don't recommend this antibody for rat in IP. In our hands rat tissues showed negative staining.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "We don't recommend this antibody for rat in WB. In our hands rat tissues showed negative staining.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "(Use 1/100 for rat tissue).Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "In IHC, under our experimental conditions, this antibody showed specific staining in bronchial epithelium cells of both rat and mouse; however, there was non-specific staining on the pulmonary alveoli of mouse lung. Our collaborator observed the correct staining on the airway epithelium of mouse lung using immunofluorescence detection method. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "We don't recommend this antibody for rat in WB. In our hands rat tissues showed negative staining.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "(Use 1/100 for rat tissue).Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "In IHC, under our experimental conditions, this antibody showed specific staining in bronchial epithelium cells of both rat and mouse; however, there was non-specific staining on the pulmonary alveoli of mouse lung. Our collaborator observed the correct staining on the airway epithelium of mouse lung using immunofluorescence detection method. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Uteroglobin antibody [EPR19846] (ab213203) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse, Rat samples.

What is the molecular weight of Uteroglobin?
Anti-Uteroglobin [EPR19846] (ab213203) specifically detects a band for Uteroglobin (UniProt: Q06318) at a molecular weight of 10kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR19846] also available for your convenience: ab213203, Carrier free - ab232562, Biotin - ab321781

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uteroglobin also known as Clara cell secretory protein (CC16) is a small secreted protein with a molecular mass of approximately 16 kDa. Expressed chiefly in the respiratory epithelium of the lungs particularly by Clara cells it is also present in other tissues such as the kidney and uterus. Uteroglobin acts as an anti-inflammatory and immunomodulatory protein playing an important role in protecting epithelial cells from damage by various harmful agents.

Biological function summary

Uteroglobin serves as a multifunctional secretory agent contributing to the regulation of inflammation and tissue remodeling. It does not form part of a larger complex but acts independently. By binding and inhibiting pro-inflammatory mediators like phospholipase A2 Uteroglobin reduces the production of arachidonic acid derivatives which are involved in inflammatory processes. Additionally it acts as a chemoattractant for certain immune cells further modulating immune responses.

Pathways

Uteroglobin integrates into the immune response and inflammation pathways. It indirectly interacts with proteins such as cytokines and chemokines influencing pathways like the arachidonic acid metabolism pathway and the leukocyte extravasation signaling pathway. These interactions reduce inflammatory response and regulate immune cell movement demonstrating its anti-inflammatory function.

Low levels of Uteroglobin have been associated with respiratory conditions such as asthma and chronic obstructive pulmonary disease (COPD). By affecting inflammation pathways Uteroglobin interacts with inflammatory cytokines which exacerbate these conditions. Additionally its connection to proteins involved in these pathways makes it a potential biomarker for assessing lung injury and monitoring disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.

See full target information Scgb1a1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013563 PubMed41052208

2025

Dysplastic lung repair fosters a tuberculosis-promoting microenvironment through maladaptive macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Shivraj M Yabaji,Suruchi Lata,Anna E Tseng,Prasanna Babu Araveti,Ming Lo,Igor Gavrish,Aoife K O'Connell,Hans P Gertje,Anna C Belkina,Colleen E Thurman,Hirofumi Kiyokawa,Darrell Kotton,Shumin Tan,Janice J Endsley,William R Bishai,Nicholas Crossland,Lester Kobzik,Igor Kramnik

STAR protocols 6:103984 PubMed40714561

2025

Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants.

Applications

Unspecified application

Species

Unspecified reactive species

Shivraj M Yabaji,Suruchi Lata,Igor Gavrish,Ming Lo,Aoife K O'Connell,Hans P Gertje,Colleen E Thurman,Nicholas A Crossland,Lester Kobzik,Igor Kramnik

iScience 28:112845 PubMed40612507

2025

Cellular heterogeneity and patterning strategies as revealed by upper respiratory epithelium single cell atlas.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander G Foote,Xin Sun

STAR protocols 6:103648 PubMed39982823

2025

Protocol for the in vivo tracing of alveolar type I cells from mice using two distinct dual recombinase-mediated genetic approaches.

Applications

Unspecified application

Species

Unspecified reactive species

Xueying Yang,Xinfeng Meng,Jingting Zhu,Zhongxiao Wang,Bin Zhou,Kuo Liu

Stem cell research & therapy 15:286 PubMed39256871

2024

Connexin 25 maintains self-renewal and functions of airway basal cells for airway regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyuan Zhang,Shaoyang Wang,Zeyu Liu,Cheng Zhong,Yuqiong Lei,Qi Zheng,Yongle Xu,Shan Shan,Hao He,Tao Ren

Nature communications 15:1496 PubMed38383468

2024

Exosomes define a local and systemic communication network in healthy pancreas and pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bárbara Adem,Nuno Bastos,Carolina F Ruivo,Sara Sousa-Alves,Carolina Dias,Patrícia F Vieira,Inês A Batista,Bruno Cavadas,Dieter Saur,José C Machado,Dawen Cai,Sonia A Melo

iScience 27:109008 PubMed38352228

2024

Circadian disruption during fetal development promotes pathological cardiac remodeling in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yu,Jing-Yu Liu,Hui-Jiao Yang,Xiao-Qin Luo,Xiao-Ping Gao,Xiao-Xin Huang,Ao-Xue Tang,Hai-Ying Mary Cheng,Wei-Chao Liu,Peng Zhang

General physiology and biophysics 43:37-48 PubMed38312033

2024

Inactivation of the TGF-β1/ALK5 axis enhances club cell function and alleviates lung tissue damage to ameliorate COPD progression through the MEK/ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Tian,Hui Ouyang,Jie Wu,Lu Wen,Xiaoping Li,Fangying Yang,Hao Yuan

Chinese medicine 18:145 PubMed37924136

2023

Therapeutic potential of Lianhua Qingke in airway mucus hypersecretion of acute exacerbation of chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanjie Hao,Tongxing Wang,Yunlong Hou,Xiaoqi Wang,Yujie Yin,Yi Liu,Ningxin Han,Yan Ma,Zhen Li,Yaru Wei,Wei Feng,Zhenhua Jia,Hui Qi

Particle and fibre toxicology 20:10 PubMed37069663

2023

Type 1 diabetes and diet-induced obesity predispose C57BL/6J mice to PM-induced lung injury: a comparative study.

Applications

Unspecified application

Species

Unspecified reactive species

Shen Chen,Miao Li,Rui Zhang,Lizhu Ye,Yue Jiang,Xinhang Jiang,Hui Peng,Ziwei Wang,Zhanyu Guo,Liping Chen,Rong Zhang,Yujie Niu,Michael Aschner,Daochuan Li,Wen Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Uteroglobin antibody [EPR19846]

Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Western blot - Anti-Uteroglobin antibody [EPR19846] (AB213203)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise