Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (AB232562)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Uteroglobin with ab213203 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Strong cytoplasmic staining on the airway epithelium of mouse lung. However, there is some non-specific cytoplasmic staining on the alveolar cells of mouse lung. The correct staining on the airway epithelium of mouse lung is observed when using immunofluorescence detection method.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213203).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (AB232562)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Uteroglobin with ab213203 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Positive staining on the airway epithelium of mouse lung [PMID : 22916196].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213203).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (AB232562)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat lung tissue labeling Uteroglobin with ab213203 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Positive staining on the airway epithelium of rat lung [PMID : 22916196].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213203).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (AB232562)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, paraffin-embedded mouse lung tissue labeling Uteroglobin antibody with ab213203 at 1/1000 dilution, followed by Donkey anti Rabbit Alexa-594 at 1/500 dilution.

Positive staining of Uteroglobin on the airway epithelium of mouse lung is observed.

The nuclear counterstain is DAPI (blue).

Heat mediated antigen retrieval using citrate buffer pH 6.0

The image was kindly provided by our collaborator Dr. Hai Song, Zhejiang University.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213203).

• IP

Supplier Data

Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] - BSA and Azide free (AB232562)

Uteroglobin was immunoprecipitated from 0.35 mg of mouse lung lysate with ab213203 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab213203 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse lung lysate 10ug (Input).

Lane 2 : ab213203 IP in mouse lung lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab213203 in mouse lung lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213203).

All lanes:

Immunoprecipitation - Anti-Uteroglobin antibody [EPR19846] (<a href='/en-us/products/primary-antibodies/uteroglobin-antibody-epr19846-ab213203'>ab213203</a>)

Predicted band size: 10 kDa

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