Anti-Uteroglobin antibody [EPR27144-86] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR27144-86] - BSA and Azide free (AB307667)

This data was developed using ab307732, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling LAGE-1 with ab307732 at 1/2000 (0.245 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human testis (PMID : 10399963). The section was incubated with ab307732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR27144-86] - BSA and Azide free (AB307667)

This data was developed using ab307732, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LAGE-1 with ab307732 at 1/2000 (0.245 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human kidney (PMID : 10399963). The section was incubated with ab307732 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uteroglobin antibody [EPR27144-86] - BSA and Azide free (AB307667)

This data was developed using ab307644, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded cell pellets with ab307666 at 1/2000 (0.271 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T (human embryonic kidney epithelial cell) transformed with a SCGB1A1 expression vector containing a his tag, no staining on (B) HEK-293T transfected with empty vector containing a his tag. The section was incubated with ab307666 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Uteroglobin antibody [EPR27144-86] - BSA and Azide free (AB307667)

This data was produced using ab307666, the same antibody clone but in a different formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells transfected with a human Uteroglobin expression vector containing a myc-his tag cells labelling Uteroglobin with ab307666 at 1/1000 dilution (0.481 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing cytoplasmic and membranous staining in HEK-293T cells transfected with a human Uteroglobin expression vector containing a myc-his tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (0.38 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Uteroglobin antibody [EPR27144-86] - BSA and Azide free (AB307667)

This data was produced using ab307666, the same antibody but in a different formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) cells transfected with a human Uteroglobin expression vector containing a myc-his tag or a control vector containing a myc-his tag cells labelling Uteroglobin with ab307666 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Uteroglobin antibody [EPR27144-86] - BSA and Azide free (AB307667)

This data was developed using ab307666, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human lung tissue staining SFTPA1 + SFTPA2 with ab320648 at a 1/1000 dilution, ab213363 anti-CD68 used at 1/500 dilution and ab307666 anti-Uteroglobin used at a 1/2000 dilution.

Panel A : merged staining of anti-SFTPA1+SFTPA2 (magenta; Opal^™690), anti-CD68 (green; Opal^™520) and anti-Uteroglobin (gray; Opal^™570) on human lung.
Panel B : anti-SFTPA1+SFTPA2 staining alveolar type II cells in human lung.
Panel C : ant-CD68 staining macrophages in human lung.
Panel D : ant-Uteroglobin staining club cells in human lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab320648, ab211363 and ab307666 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins..

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Uteroglobin antibody [EPR27144-86] - BSA and Azide free (AB307667)

This data was developed using ab307666, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human lung tissue staining Prosurfactant Protein C with ab312850 at a 1 : 2000 (0.26 ug/ml) dilution; Uteroglobin with ab307666 at 1 : 2000 dilution and MARCO with ab314646 at 1 : 100 (5.1 ug/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-Prosurfactant Protein C (magenta; Opal™690), anti-Uteroglobin (green; Opal™520) and anti-MARCO (gray; Opal™570) on human lung.
Panel B : anti-Prosurfactant Protein C staining pneumocytes in human lung.
Panel C : anti-Uteroglobin staining club cells in human lung.
Panel D : anti-MARCO staining alveolar macrophages in human lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab312850, ab307666 and ab314646 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins