Rabbit Polyclonal UTP18 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human UTP18 aa 50-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human UTP18 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5J1
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Target data
Function
Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA.
Alternative names
WDR50, CDABP0061, CGI-48, UTP18, U3 small nucleolar RNA-associated protein 18 homolog, WD repeat-containing protein 50
Recommended products
Rabbit Polyclonal UTP18 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human UTP18 aa 50-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human UTP18 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5J1
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab241578 was affinity purified using an epitope specific to UTP18 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UTP18 also known as U3 small nucleolar RNA-associated protein 18 plays a mechanical role in the assembly and maturation of small subunit (SSU) ribosomal RNA. UTP18 with an approximate mass of 35 kDa functions as part of the UTP-B module of the SSU processome. It is expressed primarily in the nucleolus of eukaryotic cells where it facilitates rRNA synthesis and processing an essential step in ribosome biogenesis.
- Biological function summary
UTP18 contributes to the early processing stages of the 18S rRNA and is integral to the formation of the SSU processome. This protein is part of a larger complex known as the small subunit (SSU) processome which contains several U3 small nucleolar RNAs and numerous protein subunits. Its activity supports the assembly of ribosomal subunits an essential function for cellular protein synthesis and growth.
- Pathways
UTP18 participates in ribosome biogenesis and nucleotide metabolism in eukaryotic cells. It associates with various proteins such as UTP14 and others within the SSU processome to ensure proper rRNA processing. UTP18 action affects the ribosome biogenesis pathway directly impacting translation and cellular metabolism.
- Associated diseases and disorders
Mutations or dysregulation of UTP18 have links to ribosomopathies which involve impaired ribosome function. Disorders such as Diamond-Blackfan anemia characterized by defective erythropoiesis may involve issues with UTP18 function. Its interactions with other SSU processome components suggest that any disruptions in this protein complex might contribute to disease pathology affecting ribosomal assembly and cellular protein synthesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-UTP18 antibody (ab241578)
All lanes: Western blot - Anti-UTP18 antibody (ab241578) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Exposure time: 1s
Immunoprecipitation - Anti-UTP18 antibody (ab241578)
UTP18 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241578 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab241578 at 0.1 μg/ml.
Lane 1: ab241578 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
Lysates prepared using NETN lysis buffer.
All lanes: Immunoprecipitation - Anti-UTP18 antibody (ab241578)
Predicted band size: 62 kDa
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