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AB241578

Anti-UTP18 antibody

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(1 Publication)

Rabbit Polyclonal UTP18 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UTP18 aa 50-150.

View Alternative Names

WDR50, CDABP0061, CGI-48, UTP18, U3 small nucleolar RNA-associated protein 18 homolog, WD repeat-containing protein 50

2 Images
Immunoprecipitation - Anti-UTP18 antibody (AB241578)
  • IP

Supplier Data

Immunoprecipitation - Anti-UTP18 antibody (AB241578)

UTP18 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241578 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab241578 at 0.1 μg/ml.

Lane 1 : ab241578 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 1 second.

Lysates prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-UTP18 antibody (ab241578)

Predicted band size: 62 kDa

false

Western blot - Anti-UTP18 antibody (AB241578)
  • WB

Supplier Data

Western blot - Anti-UTP18 antibody (AB241578)

All lanes:

Western blot - Anti-UTP18 antibody (ab241578) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 62 kDa

true

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human UTP18 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5J1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab241578 was affinity purified using an epitope specific to UTP18 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UTP18 also known as U3 small nucleolar RNA-associated protein 18 plays a mechanical role in the assembly and maturation of small subunit (SSU) ribosomal RNA. UTP18 with an approximate mass of 35 kDa functions as part of the UTP-B module of the SSU processome. It is expressed primarily in the nucleolus of eukaryotic cells where it facilitates rRNA synthesis and processing an essential step in ribosome biogenesis.
Biological function summary

UTP18 contributes to the early processing stages of the 18S rRNA and is integral to the formation of the SSU processome. This protein is part of a larger complex known as the small subunit (SSU) processome which contains several U3 small nucleolar RNAs and numerous protein subunits. Its activity supports the assembly of ribosomal subunits an essential function for cellular protein synthesis and growth.

Pathways

UTP18 participates in ribosome biogenesis and nucleotide metabolism in eukaryotic cells. It associates with various proteins such as UTP14 and others within the SSU processome to ensure proper rRNA processing. UTP18 action affects the ribosome biogenesis pathway directly impacting translation and cellular metabolism.

Mutations or dysregulation of UTP18 have links to ribosomopathies which involve impaired ribosome function. Disorders such as Diamond-Blackfan anemia characterized by defective erythropoiesis may involve issues with UTP18 function. Its interactions with other SSU processome components suggest that any disruptions in this protein complex might contribute to disease pathology affecting ribosomal assembly and cellular protein synthesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA.
See full target information UTP18
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2307660 PubMed38491910

2024

Activation of Bivalent Gene POU4F1 Promotes and Maintains Basal-like Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Zhang,Nanyan Miao,Liyan Lao,Wen Deng,Jiawen Wang,Xiaofeng Zhu,Yongsheng Huang,Huayue Lin,Wenfeng Zeng,Wei Zhang,Luyuan Tan,Xiaoqing Yuan,Xin Zeng,Jingkun Zhu,Xueman Chen,Erwei Song,Linbin Yang,Yan Nie,Di Huang
View all publications
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Product promise

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