Mouse Monoclonal UTP3 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UTP3.
Images
![Western blot - Anti-UTP3 antibody [2950C2a] (AB60063), expandable thumbnail](https://content.abcam.com/products/images/utp3-antibody-2950c2a-ab60063--western-blot-img96985.gif "Western blot - Anti-UTP3 antibody [2950C2a] (AB60063)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride, 0.0204% Potassium phosphate monobasic- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human UTP3. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQZ2
Reactivity data
Select an application| WB | |
|---|---|
| Recombinant fragment - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Essential for gene silencing: has a role in the structure of silenced chromatin. Plays a role in the developing brain (By similarity). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Alternative names
CRLZ1, SAS10, UTP3, Something about silencing protein 10, Charged amino acid-rich leucine zipper 1, Disrupter of silencing SAS10, UTP3 homolog, CRL1
Recommended products
Mouse Monoclonal UTP3 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UTP3.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride, 0.0204% Potassium phosphate monobasic- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human UTP3. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQZ2
- Clone number
- 2950C2a
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
ab60063 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UTP3 also known as Utp3p is a component of the small subunit processome involved in ribosome biogenesis. This protein has a mass of approximately 65 kDa and mainly localizes in the nucleolus of eukaryotic cells. UTP3 plays an important role in the early steps of 18S rRNA processing as part of the preribosomal particle assembly. This early involvement is essential for the production of the small ribosomal subunit which impacts protein synthesis.
- Biological function summary
UTP3 engages in ribosome assembly interacting with other proteins within the small subunit (SSU) processome complex. This complex processes pre-rRNA into mature rRNA critical for ribosome function. UTP3's role ensures the accuracy and efficiency of rRNA folding and processing which is essential for the assembly of functional ribosomal units in eukaryotic cells.
- Pathways
UTP3 is highly involved in the ribosome biogenesis pathway. This pathway is critical for cell growth and proliferation as it directly affects translation and protein synthesis. UTP3 associates with other SSU processome proteins like UTP4 and UTP15 facilitating pre-rRNA processing and ribosome assembly. These interactions highlight UTP3's importance in maintaining the integrity of the protein synthesis machinery.
- Associated diseases and disorders
Abnormalities in UTP3 function can relate to ribosomopathies and cancer. Disruptions in ribosome biogenesis involving proteins like UTP3 can lead to improper cell growth and proliferation often noted in cancers. Additionally any aberration in its interaction with other nucleolar proteins like UTP4 may further impact disease manifestation reflecting UTP3's significance in maintaining cellular homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-UTP3 antibody [2950C2a] (ab60063)
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
All lanes: Western blot - Anti-UTP3 antibody [2950C2a] (ab60063)
All lanes: immunising recombinant protein
Predicted band size: 55 kDa
Observed band size: 39 kDa
Downloads
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