JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB264258

Anti-UVRAG antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal UVRAG antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human UVRAG aa 600 to C-terminus.

View Alternative Names

UV radiation resistance-associated gene protein, p63, UVRAG

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UVRAG antibody (AB264258)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UVRAG antibody (AB264258)

Formalin-fixed, parrafin-embedded human colon carcinoma tissue stained for UVRAG using ab264258 at 1/200 dilution in immunohistochemical analysis. Detection : DAB.

Immunoprecipitation - Anti-UVRAG antibody (AB264258)
  • IP

Supplier Data

Immunoprecipitation - Anti-UVRAG antibody (AB264258)

UVRAG was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264258 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitates using ab264258 at 0.4 μg/ml.

Lane 1 : ab264258 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in Hela whole cell lysate.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-UVRAG antibody (ab264258)

Predicted band size: 78 kDa

false

Western blot - Anti-UVRAG antibody (AB264258)
  • WB

Unknown

Western blot - Anti-UVRAG antibody (AB264258)

All lanes:

Western blot - Anti-UVRAG antibody (ab264258) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 78 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human UVRAG aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P2Y5

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB124762

Anti-p63 antibody [EPR5701]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p63 antibody [EPR5701] (AB124762)

  • 4
  • 12
Primary Antibodies

AB124905

Anti-VPS34 antibody [EPR5301]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS34 antibody [EPR5301] (AB124905)

  • 0
  • 0
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UVRAG also known as UV radiation resistance-associated gene is a protein with a molecular weight of approximately 70 kDa. This protein exists within various tissues but shows higher expression in the brain heart and skeletal muscle. The mechanistic role of UVRAG involves its interaction with cellular membranes mediating autophagy and endocytic trafficking processes which are essential for cellular homeostasis and defense against stress.
Biological function summary

UVRAG regulates important cellular processes contributing to autophagy by promoting the maturation of autophagosomes into lysosomes. It is an integral part of the PI3K complex working alongside other proteins such as Beclin 1. Through these interactions UVRAG aids cell survival under nutrient-deficient conditions by facilitating the degradation of damaged organelles and protein recycling.

Pathways

UVRAG's role extends to its involvement in the mTOR signaling pathway and the endocytic pathway. Within the mTOR pathway UVRAG interacts with proteins like Rubicon and Beclin 1 acting as a modulator of cellular growth and response to energy status. In the endocytic pathway UVRAG collaborates with the VPS34 complex influencing the sorting and degradation of cellular cargo. These pathways highlight UVRAG's importance in cellular metabolism and regulation.

UVRAG shows significant connections particularly in cancer and neurodegenerative diseases. Mutations or altered expressions of UVRAG can contribute to tumorigenesis by disrupting autophagic balance or fail in cell death processes. In cancer UVRAG interacts with proteins such as p53 influencing tumor suppressor activities. In neurodegenerative disorders its regulatory role in autophagy becomes impaired contributing to protein aggregation and cell death highlighting UVRAG as a potential therapeutic target.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed : 16799551, PubMed : 18552835, PubMed : 20643123, PubMed : 24056303, PubMed : 28306502). During autophagy acts as a regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3 (PubMed : 16799551, PubMed : 20643123, PubMed : 24056303, PubMed : 28306502). Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2 (PubMed : 16799551, PubMed : 20643123, PubMed : 24056303). Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events (PubMed : 18552835, PubMed : 28306502). Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion (PubMed : 24550300). In case of negative-strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly (PubMed : 24550300).. Involved in maintaining chromosomal stability. Promotes DNA double-strand break (DSB) repair by association with DNA-dependent protein kinase complex DNA-PK and activating it in non-homologous end joining (NHEJ) (PubMed : 22542840). Required for centrosome stability and proper chromosome segregation (PubMed : 22542840).
See full target information UVRAG
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com