Rabbit Polyclonal UVRAG antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human UVRAG aa 600 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human UVRAG aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P2Y5
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
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Target data
Function
Versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed:16799551, PubMed:18552835, PubMed:20643123, PubMed:24056303, PubMed:28306502). During autophagy acts as a regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3 (PubMed:16799551, PubMed:20643123, PubMed:24056303, PubMed:28306502). Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2 (PubMed:16799551, PubMed:20643123, PubMed:24056303). Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events (PubMed:18552835, PubMed:28306502). Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion (PubMed:24550300). In case of negative-strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly (PubMed:24550300). Involved in maintaining chromosomal stability. Promotes DNA double-strand break (DSB) repair by association with DNA-dependent protein kinase complex DNA-PK and activating it in non-homologous end joining (NHEJ) (PubMed:22542840). Required for centrosome stability and proper chromosome segregation (PubMed:22542840).
Alternative names
UV radiation resistance-associated gene protein, p63, UVRAG
Recommended products
Rabbit Polyclonal UVRAG antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human UVRAG aa 600 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human UVRAG aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P2Y5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UVRAG also known as UV radiation resistance-associated gene is a protein with a molecular weight of approximately 70 kDa. This protein exists within various tissues but shows higher expression in the brain heart and skeletal muscle. The mechanistic role of UVRAG involves its interaction with cellular membranes mediating autophagy and endocytic trafficking processes which are essential for cellular homeostasis and defense against stress.
- Biological function summary
UVRAG regulates important cellular processes contributing to autophagy by promoting the maturation of autophagosomes into lysosomes. It is an integral part of the PI3K complex working alongside other proteins such as Beclin 1. Through these interactions UVRAG aids cell survival under nutrient-deficient conditions by facilitating the degradation of damaged organelles and protein recycling.
- Pathways
UVRAG's role extends to its involvement in the mTOR signaling pathway and the endocytic pathway. Within the mTOR pathway UVRAG interacts with proteins like Rubicon and Beclin 1 acting as a modulator of cellular growth and response to energy status. In the endocytic pathway UVRAG collaborates with the VPS34 complex influencing the sorting and degradation of cellular cargo. These pathways highlight UVRAG's importance in cellular metabolism and regulation.
- Associated diseases and disorders
UVRAG shows significant connections particularly in cancer and neurodegenerative diseases. Mutations or altered expressions of UVRAG can contribute to tumorigenesis by disrupting autophagic balance or fail in cell death processes. In cancer UVRAG interacts with proteins such as p53 influencing tumor suppressor activities. In neurodegenerative disorders its regulatory role in autophagy becomes impaired contributing to protein aggregation and cell death highlighting UVRAG as a potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-UVRAG antibody (ab264258)
All lanes: Western blot - Anti-UVRAG antibody (ab264258) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 78 kDa
Exposure time: 10s
Immunoprecipitation - Anti-UVRAG antibody (ab264258)
UVRAG was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264258 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitates using ab264258 at 0.4 μg/ml.
Lane 1: ab264258 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-UVRAG antibody (ab264258)
Predicted band size: 78 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UVRAG antibody (ab264258)
Formalin-fixed, parrafin-embedded human colon carcinoma tissue stained for UVRAG using ab264258 at 1/200 dilution in immunohistochemical analysis. Detection: DAB.
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