Anti-UVRAG antibody [EPR27025-83]

• WB

Supplier Data

Western blot - Anti-UVRAG antibody [EPR27025-83] (AB313626)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The identity of the higher MW band at approximately 240 kDa and the bands between 25 kDa and 60 kDa are unknown. Exposure time : 59 seconds

All lanes:

Western blot - Anti-UVRAG antibody [EPR27025-83] (ab313626) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 90 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-UVRAG antibody [EPR27025-83] (AB313626)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : HCT 116, RKO (PMID : 26234763). The identity of the higher MW band at approximately 240 kDa is unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : 37 seconds

All lanes:

Western blot - Anti-UVRAG antibody [EPR27025-83] (ab313626) at 1/1000 dilution

Lane 1:

Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 20 µg

Lane 2:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

RKO (human colon carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 90 kDa

false

Exposure time: 37s

• WB

Lab

Western blot - Anti-UVRAG antibody [EPR27025-83] (AB313626)

Western blot : Anti-UVRAG antibody [EPR27025-83] (ab313626) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab313626 was shown to bind specifically to UVRAG. A band was observed at 78-100 kDa in wild-type MCF7 cell lysates with no signal observed at this size in UVRAG knockout cell line. To generate this image, wild-type and UVRAG knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-UVRAG antibody [EPR27025-83] (ab313626) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

UVRAG knockout MCF7 cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

HEK-293T Nuclear cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 78-100 kDa

false

• WB

Supplier Data

Western blot - Anti-UVRAG antibody [EPR27025-83] (AB313626)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The identity of the higher MW band at approximately 240 kDa and the bands between 10 kDa and 75 kDa are unknown. Exposure time : Lanes 1-4 : 136 seconds; Lanes 5-7 : 92 seconds; Lane 8 : 37 seconds.

All lanes:

Western blot - Anti-UVRAG antibody [EPR27025-83] (ab313626) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Lane 7:

Rat testis tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 90 kDa

false