AB77483

Anti-UXT antibody

Name: Anti-UXT antibody (ab77483) | Abcam
Brand: Abcam Limited
SKU: AB77483
Price: 535 USD
Availability: InStock

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(2 Publications)

Goat Polyclonal UXT antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human UXT.

View Alternative Names

HSPC024, UXT, Protein UXT, Androgen receptor trapped clone 27 protein, Ubiquitously expressed transcript protein, ART-27

1 Images

Unknown

Western blot - Anti-UXT antibody (AB77483)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-UXT antibody (ab77483) at 0.03 µg/mL

All lanes:

Human Kidney lysate (in RIPA buffer) at 35 µg

Predicted band size: 18 kDa

Observed band size: 20 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human UXT. The exact immunogen used to generate this antibody is proprietary information.

Q9UBK9

Specificity

ab77483 is expected to recognize both reported isoforms (NP_004173.1; NP_705582.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab77483 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The UXT protein also known as ubiquitously expressed transcript protein plays a role in cellular homeostasis by interacting with nucleic acids and other proteins. The mass of UXT is approximately 18 kDa. It expresses ubiquitously across various tissues indicating its fundamental role in basic cellular processes. UXT involves itself in several cellular functions providing stability and immunity at the cellular level.

Biological function summary

This protein participates in transcription regulation by forming part of the larger transcriptional coregulator complex. UXT enhances the transcriptional machinery's efficiency impacting the expression of genes critical for cell cycle and survival. It coordinates with heat shock proteins possibly preventing protein denaturation and other stress responses. Its involvement across different cell types emphasizes its multifaceted role in maintaining cellular integrity.

Pathways

The UXT protein impacts transcriptional regulation and stress response pathways. It directly influences the NF-kB signaling pathway which is important for immune response and inflammation. The UXT protein also interacts with components like RelA/p65 modulating inflammatory gene expression. Moreover UXT integrates into the androgen receptor signaling pathway linking it to hormonal regulation and reproductive biology. Its connections in these pathways highlight its regulatory potential in diverse biological contexts.

UXT has associations with cancer and neurodegenerative diseases. Altered UXT expression links to certain carcinomas suggesting its role in tumorigenesis through transcriptional deregulation. UXT's interaction with proteins like p53 which influences cell cycle and apoptosis can affect cancer progression. Additionally UXT plays a part in neurodegenerative disease mechanisms although the exact pathways remain less defined. UXT's involvement in protein folding and preventing protein denaturing might explain its connection to neurodegenerative processes where misfolded proteins contribute to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in gene transcription regulation (PubMed : 21730289, PubMed : 28106301). Acts in concert with the corepressor URI1 to regulate androgen receptor AR-mediated transcription (PubMed : 11854421, PubMed : 21730289). Together with URI1, associates with chromatin to the NKX3-1 promoter region (PubMed : 21730289). Negatively regulates the transcriptional activity of the estrogen receptor ESR1 by inducing its translocation into the cytoplasm (PubMed : 28106301). May act as nuclear chaperone that facilitates the formation of the NF-kappa-B enhanceosome and thus positively regulates NF-kappa-B transcription activity (PubMed : 17620405, PubMed : 21307340). Potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling (PubMed : 17554592). Increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC (PubMed : 17554592). Suppresses cell transformation and it might mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM (PubMed : 17635584).. Isoform 1. Plays a role in protecting cells against TNF-alpha-induced apoptosis by preventing the recruitment of FADD and caspase 8 to the apoptotic complex I, composed of TRADD, TRAF2 and RIPK1/RIP.

See full target information UXT

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastroenterology and hepatology 38:2006-2017 PubMed37608570

2023

Regulation of epithelial cell differentiation by the Ubiquitous expressed transcript isoform 1 in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wu Peng,Chengcheng Zeng,Jing Xu,Hailan Zhao,Qingqing Zhu,Haoming Xu,Huiting Chen,Hongli Huang,Yongjian Zhou,Chong Zhao

Scientific reports 6:27401 PubMed27264542

2016

Specific threonine-4 phosphorylation and function of RNA polymerase II CTD during M phase progression.

Applications

Unspecified application

Species

Unspecified reactive species

Corinna Hintermair,Kirsten Voß,Ignasi Forné,Martin Heidemann,Andrew Flatley,Elisabeth Kremmer,Axel Imhof,Dirk Eick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-UXT antibody

Western blot - Anti-UXT antibody (AB77483)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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