AB188748

Anti-V2R antibody

Name: Anti-V2R antibody (ab188748) | Abcam
Brand: Abcam Limited
SKU: AB188748
Rating: 5 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal V2R antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human AVPR2.

View Alternative Names

ADHR, DIR, DIR3, V2R, AVPR2, Vasopressin V2 receptor, AVPR V2, Antidiuretic hormone receptor, Renal-type arginine vasopressin receptor

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody (AB188748)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody (AB188748)

Immunohistochemical analysis of Human kidney, renal tubules labeling V2R using ab188748 at 26 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human AVPR2. The exact immunogen used to generate this antibody is proprietary information.

P30518

Specificity

BLAST analysis of the peptide immunogen showed no homology with other human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "26 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Baboon": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The V2R or vasopressin receptor 2 is a G protein-coupled receptor with a mass of approximately 40 kDa. It is also known as the antidiuretic hormone receptor. Most expression of V2R occurs in the renal collecting ducts specifically on the basolateral surface of cells. This receptor mainly binds to arginine vasopressin an antidiuretic hormone facilitating the reabsorption of water in the kidneys.

Biological function summary

V2R plays an important role in maintaining water balance and homeostasis within the body. It is not a part of a larger complex but interacts with adenylate cyclase through the Gs protein pathway promoting cAMP generation. This increase in cAMP levels leads to the insertion of aquaporin-2 channels into the apical membrane enhancing water permeability and therefore water reabsorption in the kidney.

Pathways

The activity of V2R closely links to water regulation and osmoregulation pathways. It interacts with proteins such as aquaporin-2 as mentioned which are key in the transport of water. V2R's function connects to other signaling pathways including those involving other G protein-coupled receptors which mediate different physiological responses.

V2R dysfunction is associated with conditions such as nephrogenic diabetes insipidus and the syndrome of inappropriate antidiuretic hormone secretion (SIADH). In nephrogenic diabetes insipidus mutations in the receptor prevent normal function leading to excessive urination. This condition often involves aquaporin-2 due to its role in the same pathway. In SIADH hyperactive V2R elicits excessive water reabsorption which can result in water retention and hyponatremia.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption.

See full target information AVPR2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Veterinary world 14:2773-2781 PubMed34903939

2021

Comparative quantitation of aquaporin-2 and arginine vasopressin receptor-2 localizations among chronic kidney disease and healthy kidney in dogs.

Applications

Unspecified application

Species

Unspecified reactive species

Pitchaya Matchimakul,Wanpitak Pongkan,Piyamat Kongtung,Raktham Mektrirat

Endocrine journal 68:839-848 PubMed33790062

2021

The usefulness of the combined high-dose dexamethasone suppression test and desmopressin stimulation test in establishing the source of ACTH secretion in ACTH-dependent Cushing's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jingtao Qiao,Jiaqi Li,Weiwei Zhang,Chun Wang,Jianwei Li,Shu Jiang,Huiwen Tan,Yaxi Chen,Hui Liu,Bowen Cai,Yerong Yu

Journal of comparative pathology 179:36-40 PubMed32958145

2020

Expression of Arginine Vasopressin Type 2 Receptor in Canine Mammary Tumours: Preliminary Results.

Applications

Unspecified application

Species

Unspecified reactive species

M A Benavente,C P Bianchi,M A Aba

Scientific reports 6:35205 PubMed27748416

2016

MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection.

Applications

Unspecified application

Species

Unspecified reactive species

Qin-Qin He,Liu-Lin Xiong,Fei Liu,Xiang He,Guo-Ying Feng,Fei-Fei Shang,Qing-Jie Xia,You-Cui Wang,De-Lu Qiu,Chao-Zhi Luo,Jia Liu,Ting-Hua Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-V2R antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V2R antibody (AB188748)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

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