AB15828

Anti-V5 tag antibody

Name: Anti-V5 tag antibody (ab15828) | Abcam
Brand: Abcam Limited
SKU: AB15828
Price: 400 USD
Availability: InStock
Rating: 4 (1 reviews)

Lab Essentials
What is this?

(1 Review)

(71 Publications)

Anti-V5 tag antibody (ab15828) is a rabbit polyclonal antibody detecting V5 tag in Western Blot, IP, ChIP.

- Over 50 publications
- Trusted since 2005

2 Images

Project

Western blot - Anti-V5 tag antibody (AB15828)

Lane 1 : Primary : ab15828, 0.5 ug/ml
Sample : Recombinant V5 tagged Groucho homolog, 20 ug
Secondary : Goat anti-Rabbit IgG, 1/10000 dilution

Lane 2 : Primary : ab15828, 0.5 ug/ml
Blocking peptide : ab15829, 1 ug/ml
Sample : Recombinant V5 tagged Groucho , 20 ug
Secondary : Goat anti-Rabbit IgG, 1/10000 dilution

All lanes:

Western blot - Anti-V5 tag antibody (ab15828) at 0.5 µg/mL

Lane 1:

Recombinant V5 tagged Groucho homolog at 20 µg

Lane 2:

Recombinant V5 tagged Groucho at 20 µg with V5 tag peptide (<a href='/en-us/products/proteins-peptides/v5-tag-peptide-ab15829'>ab15829</a>)

Secondary

All lanes:

Goat anti-Rabbit IgG at 1/10000 dilution

Observed band size: 25 kDa

false

ChIP

Unknown

ChIP - Anti-V5 tag antibody (AB15828)

A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

WB, ChIP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p>ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-V5 tag antibody (ab15828) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ChIP. Tagged protein detection
V5 tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trusted by the scientific community
Anti-V5 tag (ab15828) was first used in a scientific publication in 2005 and has been cited over 50 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.

Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information V5 tag

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Publications (71)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8726 PubMed41028714

2025

Regulation of NSL by TAF4A is critical for genome stability and quiescence of muscle stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Angelina M Georgieva,Krishna Sreenivasan,Dong Ding,Clementine Villeneuve,Sara A Wickström,Stefan Günther,Carsten Kuenne,Ulrich Gärtner,Xinyue Guo,Yonggang Zhou,Xuejun Yuan,Thomas Braun

Cell death & disease 16:559 PubMed40707487

2025

RB1 controls differentiation through positive regulation of phosphoglycerate mutases.

Applications

Unspecified application

Species

Unspecified reactive species

Susumu Kohno,Nobuyuki Okahashi,Yuansong Wan,Hai Yu,Yujiro Takegami,Paing Linn,Naoko Nagatani,Shunsuke Kitajima,Teruo Kawada,Fumio Matsuda,Hiroshi Shimizu,Chiaki Takahashi

Nature communications 16:2244 PubMed40050279

2025

SART3 promotes homologous recombination repair by stimulating DNA-RNA hybrids removal and DNA end resection.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Fu,Min Huang,Honglin Wu,Hui Zheng,Yifei Gong,Lingyu Xing,Juanjuan Gong,Ruiyuan An,Qian Li,Xinyu Jie,Xiaolu Ma,Tie-Shan Tang,Caixia Guo

Orphanet journal of rare diseases 20:57 PubMed39920764

2025

Deciphering TCOF1 mutations in Chinese Treacher Collins syndrome patients: insights into pathogenesis and transcriptional disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoyuan Jiang,Ke Mao,Bingqing Wang,Hao Zhu,Jiqiang Liu,Ruirui Lang,Baichuan Xiao,Hailin Shan,Qi Chen,Ying Li,Shouqin Zhao,Qingguo Zhang,Huisheng Liu,Yong-Biao Zhang

iScience 27:111452 PubMed39720519

2024

A negatively charged region within carboxy-terminal domain maintains proper CTCF DNA binding.

Applications

Unspecified application

Species

Unspecified reactive species

Lian Liu,Yuanxiao Tang,Yan Zhang,Qiang Wu

Nature communications 15:10186 PubMed39582024

2024

Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuteng Weng,Yanhuizhi Feng,Zeyuan Li,Shuyu Xu,Di Wu,Jie Huang,Haicheng Wang,Zuolin Wang

Scientific reports 14:27425 PubMed39521917

2024

AEBP1 is a negative regulator of skeletal muscle cell differentiation in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fumika Okazaki,Akira Yorozu,Shohei Sekiguchi,Takeshi Niinuma,Reo Maruyama,Hiroshi Kitajima,Eiichiro Yamamoto,Kazuya Ishiguro,Mutsumi Toyota,Yui Hatanaka,Koyo Nishiyama,Kazuhiro Ogi,Masahiro Kai,Kenichi Takano,Shingo Ichimiya,Akihiro Miyazaki,Hiromu Suzuki

JCI insight 9: PubMed39499902

2024

BPDCN MYB fusions regulate cell cycle genes, impair differentiation, and induce myeloid-dendritic cell leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher A G Booth,Juliette M Bouyssou,Katsuhiro Togami,Olivier Armand,Hembly G Rivas,Kezhi Yan,Siobhan Rice,Shuyuan Cheng,Emily M Lachtara,Jean-Pierre Bourquin,Alex Kentsis,Esther Rheinbay,James A DeCaprio,Andrew A Lane

Journal of virology 98:e0082624 PubMed39194246

2024

Human papillomavirus 16 replication converts SAMHD1 into a homologous recombination factor and promotes its recruitment to replicating viral DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Claire D James,Aya Youssef,Apurva T Prabhakar,Raymonde Otoa,Jenny D Roe,Austin Witt,Rachel L Lewis,Molly L Bristol,Xu Wang,Kun Zhang,Renfeng Li,Iain M Morgan

Science advances 10:eadm8196 PubMed38787953

2024

Human DNA topoisomerase I poisoning causes R loop-mediated genome instability attenuated by transcription factor IIS.

Applications

Unspecified application

Species

Unspecified reactive species

Renée C Duardo,Jessica Marinello,Marco Russo,Sara Morelli,Simona Pepe,Federico Guerra,Belén Gómez-González,Andrés Aguilera,Giovanni Capranico

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-V5 tag antibody

Western blot - Anti-V5 tag antibody (AB15828)

ChIP - Anti-V5 tag antibody (AB15828)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (71)

Product promise