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AB9116

Anti-V5 tag antibody

4

(13 Reviews)

|

(187 Publications)

Anti-V5 tag antibody (ab9116) is a rabbit polyclonal antibody detecting V5 tag in Western Blot, IP, IHC-P, ELISA.

- Over 160 publications
- Trusted since 2002
1 Images
Western blot - Anti-V5 tag antibody (AB9116)
  • WB

AbReview13363****

Western blot - Anti-V5 tag antibody (AB9116)

Mouse ES cells lysate.

All lanes:

Western blot - Anti-V5 tag antibody (ab9116) at 1/5000 dilution

Lanes 1 - 3:

Cells transfected with v5-tagged protein.

Lanes 4 - 5:

Untransfected cells.

Secondary

All lanes:

Anti-rabbit Ig-HRP at 1/20000 dilution

false

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

No

Applications

IHC-P, IP, WB, ELISA

applications

Specificity

ELISA: V5 tag antibody - ChIP Grade (ab9116) specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:50,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP- conjugated goat anti rabbit (IgG) at 1:10000 and TMB as the substrate. Appropriate specificity controls were run.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-V5 tag antibody (ab9116) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ELISA. Tagged protein detection
V5 tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trusted by the scientific community
Anti-V5 tag (ab9116) was first used in a scientific publication in 2002 and has been cited over 160 times in peer-reviewed journals.

Reviewed by scientists
Anti-V5 tag (ab9116) has over 10 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg .
Storage buffer
pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.
Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information V5 tag
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Publications (187)

Recent publications for all applications. Explore the full list and refine your search

Nature 647:517-527 PubMed40866703

2025

Mechanical confinement governs phenotypic plasticity in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Miranda V Hunter,Eshita Joshi,Sydney Bowker,Emily Montal,Yilun Ma,Young Hun Kim,Zhifan Yang,Laura Tuffery,Zhuoning Li,Eric Rosiek,Alexander Browning,Reuben Moncada,Itai Yanai,Helen Byrne,Mara Monetti,Elisa de Stanchina,Pierre-Jacques Hamard,Richard P Koche,Richard M White

Science advances 11:eado3852 PubMed39841822

2025

The deubiquitinase USP5 prevents accumulation of protein aggregates in cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yvonne Eibach,Silke Kreher,Mareike S Poetsch,Ay Lin Kho,Ulrich Gaertner,Christoph S Clemen,Rolf Schröder,Kai Guo,Hendrik Milting,Benjamin Meder,Michael Potente,Manfred Richter,Andre Schneider,Silke Meiners,Mathias Gautel,Thomas Braun

eLife 12: PubMed39792435

2025

Surprising features of nuclear receptor interaction networks revealed by live-cell single-molecule imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Liza Dahal,Thomas G W Graham,Gina M Dailey,Alec Heckert,Robert Tjian,Xavier Darzacq

eLife 12: PubMed39773557

2025

Complexes of vertebrate TMC1/2 and CIB2/3 proteins form hair-cell mechanotransduction cation channels.

Applications

Unspecified application

Species

Unspecified reactive species

Arnaud P J Giese,Wei-Hsiang Weng,Katie S Kindt,Hui Ho Vanessa Chang,Jonathan S Montgomery,Evan M Ratzan,Alisha J Beirl,Roberto Aponte Rivera,Jeffrey M Lotthammer,Sanket Walujkar,Mark P Foster,Omid A Zobeiri,Jeffrey R Holt,Saima Riazuddin,Kathleen E Cullen,Marcos Sotomayor,Zubair M Ahmed

Nature immunology 25:2308-2319 PubMed39424985

2024

A SIRT7-dependent acetylation switch regulates early B cell differentiation and lineage commitment through Pax5.

Applications

Unspecified application

Species

Unspecified reactive species

Andres Gamez-Garcia,Maria Espinosa-Alcantud,Alberto Bueno-Costa,Elisenda Alari-Pahissa,Anna Marazuela-Duque,Joshua K Thackray,Chandni Ray,Clara Berenguer,Poonam Kumari,Joan Josep Bech,Thomas Braun,Alessandro Ianni,Jay A Tischfield,Lourdes Serrano,Manel Esteller,Jose L Sardina,Carolina De La Torre,Mikael Sigvardsson,Berta N Vazquez,Alejandro Vaquero

Immunity 57:1908-1922.e6 PubMed39079535

2024

Cancer stem cells release interleukin-33 within large oncosomes to promote immunosuppressive differentiation of macrophage precursors.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah L Erickson,Sachiko Taniguchi,Anish Raman,Justin J Leitenberger,Sanjay V Malhotra,Naoki Oshimori

Nature biomedical engineering 8:1124-1141 PubMed38698155

2024

Transient pacing in pigs with complete heart block via myocardial injection of mRNA coding for the T-box transcription factor 18.

Applications

Unspecified application

Species

Unspecified reactive species

David W Wolfson,Nam Kyun Kim,Ki Hong Lee,Jared P Beyersdorf,Jonathan J Langberg,Natasha Fernandez,Dahim Choi,Nadine Zureick,Tae Yun Kim,Seongho Bae,Jin-Mo Gu,Jonathan L Kirschman,Jinqi Fan,Christina Y Sheng,Danielle Gottlieb Sen,Bret Mettler,Jung Hoon Sung,Young-Sup Yoon,Sung-Jin Park,Philip J Santangelo,Hee Cheol Cho

Cell reports 43:114054 PubMed38578832

2024

14-3-3 binding motif phosphorylation disrupts Hdac4-organized condensates to stimulate cardiac reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Liu,Ienglam Lei,Shuo Tian,Wenbin Gao,Yijing Guo,Zhaokai Li,Ziad Sabry,Paul Tang,Y Eugene Chen,Zhong Wang

The Journal of biological chemistry 300:107206 PubMed38519031

2024

Dual-specificity kinase DYRK3 phosphorylates p62 at the Thr-269 residue and promotes melanoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Hyung Lee,A-Rum Yoon,Chae-Ok Yun,Kwang Chul Chung

The Journal of biological chemistry 300:107198 PubMed38508312

2024

The SCF-FBW7β E3 ligase mediates ubiquitination and degradation of the serine/threonine protein kinase PINK1.

Applications

Unspecified application

Species

Unspecified reactive species

Seo Jeong Jeon,Kwang Chul Chung
View all publications
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Product promise

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