Goat Polyclonal V5 tag antibody. Suitable for ICC, ELISA, WB, IHC-P and reacts with Tag samples. Cited in 13 publications.
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- Frontiers in neuroscience 17:12446792023Retinal astrocyte morphology predicts integration of vascular and neuronal architecture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph M Holden,Lauren K Wareham,David J CalkinsPubMed 37621717
- Nature communications 13:8902022Pharmacological perturbation of CXCL1 signaling alleviates neuropathogenesis in a model of HEVA71 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaravanan Gunaseelan,Mohammed Zacky Ariffin,Sanjay Khanna,Mong How Ooi,David Perera,Justin Jang Hann Chu,John Jia En ChuaPubMed 35173169
- Frontiers in pharmacology 12:7144522021Assessment of Lentiviral Vector Mediated CFTR Correction in Mice Using an Improved Rapid Nasal Potential Difference Measurement Protocol.Applications:Unspecified applicationReactive species:Unspecified reactive speciesP Cmielewski,J Delhove,M Donnelley,D ParsonsPubMed 34385926
- Frontiers in pharmacology 12:6822992021Single-Dose Lentiviral Mediated Gene Therapy Recovers CFTR Function in Cystic Fibrosis Knockout Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicole Reyne,Patricia Cmielewski,Alexandra McCarron,Juliette Delhove,David Parsons,Martin DonnelleyPubMed 34084147
- Biochimica et biophysica acta. Molecular basis of disease 1867:1661062021Predominant cellular mitochondrial dysfunction in the TOP3A gene-caused Bloom syndrome-like disorder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjun Jiang,Nan Jia,Chaowan Guo,Juan Wen,Lingqian Wu,Tomoo Ogi,Huiwen ZhangPubMed 33631320
- Scientific reports 10:71382020Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShimaa H A Soliman et. al.PubMed 32346083
- Cell 179:251-267.e242019Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGi Bum Kim,David Rincon Fernandez Pacheco,David Saxon,Amy Yang,Sara Sabet,Marina Dutra-Clarke,Rachelle Levy,Ashley Watkins,Hannah Park,Aslam Abbasi Akhtar,Paul W Linesch,Naomi Kobritz,Swasty S Chandra,Katie Grausam,Alberto Ayala-Sarmiento,Jessica Molina,Kristyna Sedivakova,Kendy Hoang,Jeremiah Tsyporin,Daniel S Gareau,Mariella G Filbin,Serguei Bannykh,Chintda Santiskulvong,Yizhou Wang,Jie Tang,Mario L Suva,Bin Chen,Moise Danielpour,Joshua J BreunigPubMed 31539496
- PLoS biology 17:e30003742019Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLech Kaczmarczyk et. al.PubMed 31393866
- Veterinary pathology 56:703-7102019Exogenous Expression of Equine MHC Class I Molecules in Mice Increases Susceptibility to Equine Herpesvirus 1 Pulmonary Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErina Minato et. al.PubMed 30866742
- Molecular therapy : the journal of the American So 23:1582-912015Riboswitch-mediated Attenuation of Transgene Cytotoxicity Increases Adeno-associated Virus Vector Yields in HEK-293 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin Strobel et. al.PubMed 26137851
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| ICC | ELISA | WB | IHC-P | |
|---|---|---|---|---|
| Tag | Expected | Expected | Tested | Tested |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes Coating: 2 - 10 ug/ml; Primary: 0.03 - 1 ug/ml |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes Colorimetric: 0.1 - 1 ug/ml; Chemiluminescent: 0.03 - 1 ug/ml |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Alternative names
GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, V5 epitope tag
Recommended products
Goat Polyclonal V5 tag antibody. Suitable for ICC, ELISA, WB, IHC-P and reacts with Tag samples. Cited in 13 publications.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.
- Biological function summary
Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.
- Pathways
The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.
- Associated diseases and disorders
Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-V5 tag antibody (ab95038)
A, cells expressing 315-HARE, 190-HARE, or EV alone were cultured in T-25 flasks until confluent, washed, scraped, and lysed. Cell lysates (100ug of protein) were immunoprecipitated (IP) with anti-Tyr(P) mAb and the immune complexes were subjected to 5% SDS-PAGE and Western blot (WB) analysis using anti-V5 pAb. Cells expressing 315-HARE (B) or 190-HARE (C) cDNA were grown until confluent, washed, incubated for 1 h in medium without serum, and then incubated with (HA) or without (Ctr) 5ug/ml HA for 20 min. The cells were lysed and 200 ug of total lysate protein was immunoprecipitated with anti-HARE mAb-30. Immune complexes were collected and subjected to 5% SDS-PAGE and Western analysis using anti- Tyr(P) mAb. The same blots in B and C were reprobed with anti-V5 Ab (D and E, respectively). Immunopurification using non-immune mouse IgG served as another control in B–E. The solid and open arrows indicate the positions of the 315-HARE and 190-HARE, respectively.
All lanes: Western blot - Anti-V5 tag antibody (ab95038)
Western blot - Anti-V5 tag antibody (ab95038)
All lanes: Western blot - Anti-V5 tag antibody (ab95038) at 0.4 µg/mL
Lane 1: E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2: E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3: E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Developed using the ECL technique.
Exposure time: 30s
Image from Faraji F et al., PLoS Genet 8(9): e1002926.. Fig 4.; doi: 10.1371/journal.pgen.1002926. Epub 2012 Sep 20.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-V5 tag antibody (ab95038)
Immunohistochemical analysis of murine mammary tumour tissue, expressing vector (left) or V5-tagged Cadm1 (right), staining V5 tag with ab95038.
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