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AB53418

Anti-V5 tag antibody [E10]

4

(6 Reviews)

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(6 Publications)

Mouse Monoclonal V5 tag antibody. Suitable for ELISA, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Tag samples. Cited in 6 publications.

View Alternative Names

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, V5 epitope tag

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [E10] (AB53418)
  • ICC/IF

AbReview39525****

Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [E10] (AB53418)

ab53418 staining V5 tag in Mouse B16F1 melanoma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Tween 20 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in 1% BSA + 0.5% Tween 20 + 1% Donkey serum) for 1 hour at 21°C. An Alexa Fluor® 555-conjugated Donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

Melonma cells expressing GFP are also expressing GFP (AF55) due to transfected cell line with GFP-V5.

This image is courtesy of an Abreview submitted by Javier Rodriguez

Western blot - Anti-V5 tag antibody [E10] (AB53418)
  • WB

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Western blot - Anti-V5 tag antibody [E10] (AB53418)

All lanes:

Western blot - Anti-V5 tag antibody [E10] (ab53418) at 1/1000 dilution

Lane 1:

293 cellsuntransfected (control) at 30 µg

Lane 2:

293 cellstransfectedwith lacZ-V5-tagged at 30 µg

Observed band size: 130 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

E10

Isotype

IgG1

Carrier free

No

Applications

IHC-Fr, IHC-P, ICC/IF, ELISA, WB

applications

Specificity

ab53418 detects (GKPIPNPLLGLDST) V5 tag fusion protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/5000 - 1/10000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/2000", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/500 - 1/2000", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab53418 was affinity purified from culture supernatant of hybridoma cells grown in a bioreactor.
Storage buffer
pH: 7.2 Preservative: 0.05% Sodium azide Constituents: 0.0268% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.
Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information V5 tag
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature 570:533-537 PubMed31217585

2019

A universal biomolecular integral feedback controller for robust perfect adaptation.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie K Aoki,Gabriele Lillacci,Ankit Gupta,Armin Baumschlager,David Schweingruber,Mustafa Khammash

Nucleic acids research 47:5381-5394 PubMed30931476

2019

Dido3-dependent SFPQ recruitment maintains efficiency in mammalian alternative splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Mora Gallardo,Ainhoa Sánchez de Diego,Julio Gutiérrez Hernández,Amaia Talavera-Gutiérrez,Thierry Fischer,Carlos Martínez-A,Karel H M van Wely

Cell 167:219-232.e14 PubMed27662090

2016

Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing.

Applications

WB

Species

Unspecified reactive species

Angelo Amabile,Alessandro Migliara,Paola Capasso,Mauro Biffi,Davide Cittaro,Luigi Naldini,Angelo Lombardo

Insect biochemistry and molecular biology 54:80-8 PubMed25240618

2014

Sumoylation modulates 20-hydroxyecdysone signaling by maintaining USP protein levels in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawan Wang,Sheng Wang,Sheng Li

Archives of toxicology 89:2027-38 PubMed25224401

2014

Novel role of hnRNP-A2/B1 in modulating aryl hydrocarbon receptor ligand sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

See-Wun Cho,Ken-ichi Suzuki,Yoshiaki Miura,Tatsuhiko Miyazaki,Masato Nose,Hisato Iwata,Eun-Young Kim

Journal of virology 84:10681-9 PubMed20702639

2010

The African swine fever virus DP71L protein recruits the protein phosphatase 1 catalytic subunit to dephosphorylate eIF2alpha and inhibits CHOP induction but is dispensable for these activities during virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Fuquan Zhang,Alice Moon,Kay Childs,Stephen Goodbourn,Linda K Dixon
View all publications
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