JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB182008

Anti-V5 tag antibody [EPR12989]

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Recombinant Monoclonal V5 tag antibody. Suitable for WB and reacts with Tag samples. Cited in 5 publications.

View Alternative Names

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, V5 epitope tag

1 Images
Western blot - Anti-V5 tag antibody [EPR12989] (AB182008)
  • WB

Supplier Data

Western blot - Anti-V5 tag antibody [EPR12989] (AB182008)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-V5 tag antibody [EPR12989] (ab182008) at 1/50000 dilution

All lanes:

multi-tag fusion protein, 5ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

false

  • Carrier free

    Anti-V5 tag antibody [EPR12989] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12989

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/50000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.
Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information V5 tag
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:269 PubMed38475767

2024

Bacterial DnaK reduces the activity of anti-cancer drugs cisplatin and 5FU.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Benedetti,Emmanuel F Mongodin,Jonathan H Badger,Arshi Munawwar,Ashley Cellini,Weirong Yuan,Giovannino Silvestri,Carl N Kraus,Simone Marini,Chozha V Rathinam,Marco Salemi,Hervé Tettelin,Robert C Gallo,Davide Zella

Proceedings of the National Academy of Sciences of the United States of America 120:e2219897120 PubMed37459550

2023

Mycoplasma DnaK increases DNA copy number variants in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Benedetti,Giovannino Silvestri,Saman Saadat,Frank Denaro,Olga S Latinovic,Harry Davis,Sumiko Williams,Joseph Bryant,Rudy Ippodrino,Chozha V Rathinam,Robert C Gallo,Davide Zella

Cell death & disease 13:532 PubMed35668069

2022

Furin extracellularly cleaves secreted PTENα/β to generate C-terminal fragment with a tumor-suppressive role.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Zhang,Hong-Ming Ma,Shuang-Shu Dong,Na Zhang,Ping He,Meng-Kai Ge,Li Xia,Jian-Xiu Yu,Qiang Xia,Guo-Qiang Chen,Shao-Ming Shen

Proceedings of the National Academy of Sciences of 117:15137-15147 PubMed32554502

2020

Pseudouridylation defect due to and mutations causes nephrotic syndrome with cataracts, hearing impairment, and enterocolitis.

Applications

Unspecified application

Species

Unspecified reactive species

Eszter Balogh,Jennifer C Chandler,Máté Varga,Mona Tahoun,Dóra K Menyhárd,Gusztáv Schay,Tomas Goncalves,Renáta Hamar,Regina Légrádi,Ákos Szekeres,Olivier Gribouval,Robert Kleta,Horia Stanescu,Detlef Bockenhauer,Andrea Kerti,Hywel Williams,Veronica Kinsler,Wei-Li Di,David Curtis,Maria Kolatsi-Joannou,Hafsa Hammid,Anna Szőcs,Kristóf Perczel,Erika Maka,Gergely Toldi,Florentina Sava,Christelle Arrondel,Magdolna Kardos,Attila Fintha,Ahmed Hossain,Felipe D'Arco,Mario Kaliakatsos,Jutta Koeglmeier,William Mifsud,Mariya Moosajee,Ana Faro,Eszter Jávorszky,Gábor Rudas,Marwa H Saied,Salah Marzouk,Kata Kelen,Judit Götze,George Reusz,Tivadar Tulassay,François Dragon,Géraldine Mollet,Susanne Motameny,Holger Thiele,Guillaume Dorval,Peter Nürnberg,András Perczel,Attila J Szabó,David A Long,Kazunori Tomita,Corinne Antignac,Aoife M Waters,Kálmán Tory

Experimental hematology 44:745-754.e4 PubMed27174803

2016

Biological implications of somatic DDX41 p.R525H mutation in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Moe Kadono,Akinori Kanai,Akiko Nagamachi,Satoru Shinriki,Jin Kawata,Koji Iwato,Taiichi Kyo,Kumi Oshima,Akihiko Yokoyama,Takeshi Kawamura,Reina Nagase,Daichi Inoue,Toshio Kitamura,Toshiya Inaba,Tatsuo Ichinohe,Hirotaka Matsui
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB18617

Biotin Anti-V5 tag antibody

  • 0
  • 0
Primary Antibodies

AB9116

Anti-V5 tag antibody

Lab Essentials

Western blot - Anti-V5 tag antibody (AB9116)

  • 4
  • 13
Primary Antibodies

AB27671

Anti-V5 tag antibody [SV5-Pk1]

Lab Essentials

Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671)

  • 5
  • 11
Primary Antibodies

AB27672

Biotin Anti-V5 tag antibody [SV5-Pk1]

  • 0
  • 0
Primary Antibodies

AB206565

Anti-V5 tag antibody [SV5-P-K]

Recombinant

ELISA - Anti-V5 tag antibody [SV5-P-K] (AB206565)

  • 0
  • 0
Primary Antibodies

AB206566

Anti-V5 tag antibody [SV5-P-K]

Recombinant

Indirect ELISA - Anti-V5 tag antibody [SV5-P-K] (AB206566)

  • 5
  • 1
Primary Antibodies

AB206571

Anti-V5 tag antibody [SV5-P-K]

Recombinant

ELISA - Anti-V5 tag antibody [SV5-P-K] (AB206571)

  • 0
  • 0
Primary Antibodies

AB206557

Anti-V5 tag antibody [SV5-P-K]

Recombinant

ELISA - Anti-V5 tag antibody [SV5-P-K] (AB206557)

  • 0
  • 0
Primary Antibodies

AB206558

Anti-V5 tag antibody [SV5-P-K]

Recombinant

  • 0
  • 0
Primary Antibodies

AB206559

Anti-V5 tag antibody [SV5-P-K]

Recombinant

  • 0
  • 0
Primary Antibodies

AB206561

Anti-V5 tag antibody [SV5-P-K]

Recombinant

  • 0
  • 0
Primary Antibodies

AB206562

Anti-V5 tag antibody [SV5-P-K]

Recombinant

  • 0
  • 0
Primary Antibodies

AB1229

Agarose Anti-V5 tag antibody

  • 5
  • 1
Primary Antibodies

AB15828

Anti-V5 tag antibody

Lab Essentials

Western blot - Anti-V5 tag antibody (AB15828)

  • 4
  • 1
Primary Antibodies

AB72560

SureLight® APC Anti-V5 tag antibody

Flow Cytometry - SureLight® APC Anti-V5 tag antibody (AB72560)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB236632

Anti-HA tag antibody [EPR22819-101]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (AB236632)

  • 3
  • 1
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com