AB206560

Anti-V5 tag antibody [SV5-P-K]

Recombinant
What is this?

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(1 Publication)

Human Recombinant Monoclonal V5 tag antibody. Suitable for IP, ELISA, WB, RIA, ICC/IF, IHC-Fr and reacts with Tag samples. Cited in 1 publication.

View Alternative Names

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, V5 epitope tag

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

SV5-P-K

Light chain type

kappa

Carrier free

Applications

WB, IHC-Fr, RIA, ELISA, ICC/IF, IP

applications

Specificity

ab206560 recognises a 14 amino acid sequence (GKPIPNPLLGLDST) derived from the P and V proteins of the paramyxovirus simian virus 5.

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Reactivity data

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Product details

ab206560 is a recombinant monoclonal antibody to V5 epitope tag, manufactured using variable regions (i.e. specificity) from the hybridoma SV5-P-K. This is a chimeric human Fab fragment with a his-tag, based on the original mouse IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified by Immobilized Metal Affinity Chromatography.

Storage buffer

Preservative: 0.02% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.

Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information V5 tag

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 223: PubMed39373700

2024

Dachsous and Fat coordinately repress the Dachs-Dlish-Approximated complex to control growth.

Applications

Unspecified application

Species

Unspecified reactive species

Hitoshi Matakatsu,Richard G Fehon

View all publications

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