Anti-V5 tag antibody [SV5-P-K]

• I-ELISA

Supplier Data

Indirect ELISA - Anti-V5 tag antibody [SV5-P-K] (AB206566)

ELISA analysis of multi-tag protein labeling V5 tag using ab206566. Plate coated with 5 μg/mL of Multi-tag protein. Primary antibody loaded at 1000 ng/mL in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated Goat anti-Mouse secondary antibody.

• WB

Lab

Western blot - Anti-V5 tag antibody [SV5-P-K] (AB206566)

Anti-V5 tag antibody [SV5-P-K] staining at 1/1000 dilution, shown in black. In Western blot, ab206566 was shown to bind specifically to V5 tag. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 3 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) at 1/50000 dilution.

All lanes:

Western blot - Anti-V5 tag antibody [SV5-P-K] (ab206566) at 1/1000 dilution

Lane 1:

Western blot - Recombinant E. coli Multi Tag protein (<a href='/en-us/products/proteins-peptides/recombinant-e-coli-multi-tag-protein-ab36791'>ab36791</a>) at 0.2 µg

Lane 2:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a>) at 0.2 µg

Observed band size: 45 kDa

false

Exposure time: 3min