Anti-V5 tag antibody [SV5-Pk1] ab27671 is a mouse monoclonal antibody that is used in V5 tag western blotting, IHC and flow cytometry.
- Antibody clone SV5-Pk1 has been tried and trusted by researchers since 2005
Same trusted quality, new lower price
Images
![Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671), expandable thumbnail](https://content.abcam.com/products/images/v5-tag-antibody-sv5-pk1-ab27671--western-blot-img138988.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671)")
![Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671), expandable thumbnail](https://content.abcam.com/products/images/v5-tag-antibody-sv5-pk1-ab27671--western-blot-img103756.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671)")
Publications
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- PLoS biology 21:e30023372023Spatacsin regulates directionality of lysosome trafficking by promoting the degradation of its partner AP5Z1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandre Pierga,Raphaël Matusiak,Margaux Cauhapé,Julien Branchu,Lydia Danglot,Maxime Boutry,Frédéric DariosPubMed 37871017
- The Journal of clinical investigation 133:2023SELENOP modifies sporadic colorectal carcinogenesis and WNT signaling activity through LRP5/6 interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer M Pilat,Rachel E Brown,Zhengyi Chen,Nathaniel J Berle,Adrian P Othon,M Kay Washington,Shruti A Anant,Suguru Kurokawa,Victoria H Ng,Joshua J Thompson,Justin Jacobse,Jeremy A Goettel,Ethan Lee,Yash A Choksi,Ken S Lau,Sarah P Short,Christopher S WilliamsPubMed 37166989
- ACS synthetic biology 12:657-6632023A Biological OR(XNOR) Logic Gate Couples Carbon Source and Transgene Expression Switching in a () Strain Co-producing Process-Enhancing Lipase and a Virus-like Particle (VLP) Vaccine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSushobhan Bandyopadhyay,Vasos Pavlika,Daniel G Bracewell,Darren N NesbethPubMed 36848292
- Frontiers in immunology 13:10162142023Duck TRIM29 negatively regulates type I IFN production by targeting MAVS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiqiang Li,Yating Song,Yuqing Du,Zhanhong Huang,Meng Zhang,Zuxian Chen,Zhuoliang He,Yangbao Ding,Junsheng Zhang,Luxiang Zhao,Hailiang Sun,Peirong JiaoPubMed 36685538
- Human molecular genetics 32:1497-15102022Characterization of the TBR1 interactome: variants associated with neurodevelopmental disorders disrupt novel protein interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElliot Sollis,Joery den Hoed,Marti Quevedo,Sara B Estruch,Arianna Vino,Dick H W Dekkers,Jeroen A A Demmers,Raymond Poot,Pelagia Deriziotis,Simon E FisherPubMed 36579832
- Cellular and molecular life sciences : CMLS 79:6152022The endocytic recycling compartment serves as a viral factory for hepatitis E virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCyrine Bentaleb,Kévin Hervouet,Claire Montpellier,Charline Camuzet,Martin Ferrié,Julien Burlaud-Gaillard,Stéphane Bressanelli,Karoline Metzger,Elisabeth Werkmeister,Maliki Ankavay,Nancy Leon Janampa,Julien Marlet,Julien Roux,Clarence Deffaud,Anne Goffard,Yves Rouillé,Jean Dubuisson,Philippe Roingeard,Cécile-Marie Aliouat-Denis,Laurence CocquerelPubMed 36460928
- Frontiers in cellular and infection microbiology 12:9260362022Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDarshika J Udawatte,Diane M Lang,Jeffrey R Currier,Carey L Medin,Alan L RothmanPubMed 36310878
- Journal of virology 96:e01278222022G3BP/Rin-Binding Motifs Inserted into Flexible Regions of nsP2 Support RNA Replication of Chikungunya Virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSainan Wang,Andres MeritsPubMed 36226983
- Science advances 8:eabq63532022Cryo-EM reveals a mechanism of USP1 inhibition through a cryptic binding site.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin L Rennie,Connor Arkinson,Viduth K Chaugule,Helen WaldenPubMed 36170365
- The Journal of biological chemistry 298:1024842022A STUB1 ubiquitin ligase/CHIC2 protein complex negatively regulates the IL-3, IL-5, and GM-CSF cytokine receptor common β chain (CSF2RB) protein stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSebastian H J Koochaki,Mikołaj Słabicki,Ryan Lumpkin,Charles Zou,Roger Belizaire,Eric S Fischer,Benjamin L EbertPubMed 36108743
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Virus preparation containing V5 tag protein. The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ELISA | WB | RIA | IHC-Fr | |
|---|---|---|---|---|---|---|
| Transfected cell lysate - Simian virus 5 (strain W3) (SV5) | Expected | Expected | Expected | Tested | Expected | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Simian virus 5 (strain W3) (SV5) | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Simian virus 5 (strain W3) (SV5) | Dilution info Use at an assay dependent concentration. | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Simian virus 5 (strain W3) (SV5) | Dilution info 1/1000 - 1/5000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Simian virus 5 (strain W3) (SV5) | Dilution info 1/1000 - 1/5000 | Notes - |
RIA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Simian virus 5 (strain W3) (SV5) | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Simian virus 5 (strain W3) (SV5) | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Alternative names
GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, V5 epitope tag
Recommended products
Anti-V5 tag antibody [SV5-Pk1] ab27671 is a mouse monoclonal antibody that is used in V5 tag western blotting, IHC and flow cytometry.
- Antibody clone SV5-Pk1 has been tried and trusted by researchers since 2005
Same trusted quality, new lower price
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Virus preparation containing V5 tag protein. The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SV5-Pk1
- Purification technique
- Affinity purification Protein A
- Epitope
- ab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
- Concentration
- Purification notes
This antibody was purified from tissue culture supernatant by affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Anti-V5 tag antibody [SV5-Pk1] (ab27671) is a mouse monoclonal antibody and is validated for use in ELISA, Flow Cyt, IHC-Fr, IP, RIA, WB in human, mouse samples.
Anti-V5 tag antibody [SV5-Pk1] (ab27671) has been cited over 153 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-V5 tag antibody [SV5-Pk1] (ab27671) has high sensitivity and specificity.
Anti-V5 tag antibody [SV5-Pk1] (ab27671) has 11 independent reviews from customers.
Anti-V5 tag antibody [SV5-Pk1] (ab27671) specifically detects V5 tag (UniProt ID: P11207; Molecular weight: 24kDa) and is sold in 100 µg selling sizes.
Antibody clone SV5-Pk1 is also available pre-conjugated to Biotin for your convenience (Biotin Anti-V5 tag antibody [SV5-Pk1] ab27672).
ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.
- Biological function summary
Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.
- Pathways
The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.
- Associated diseases and disorders
Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671), expandable thumbnail](https://content.abcam.com/products/images/v5-tag-antibody-sv5-pk1-ab27671--western-blot-img138988.jpg "Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)")
Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)
Cells were transfected with a V5-tagged HSPB8 (MW:~25kD) plasmid, which expressed a V5-tagged protein with molecular weight of approximately 28kD.
All lanes: Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution
Lanes 1 - 2: Transfected NIH/3T3 cell lysate
Lane 3: Not transfected NIH/3T3 cell lysate
SecondaryAll lanes: HRP-conjugated rabbit polyclonal Anti-mouse
Developed using the ECL technique.
Exposure time: 1s
![Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671), expandable thumbnail](https://content.abcam.com/products/images/v5-tag-antibody-sv5-pk1-ab27671--western-blot-img103756.jpg "Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)")
Flores et al PLoS One. 2016 Oct 24;11(10):e0165084. doi: 10.1371/journal.pone.0165084. eCollection 2016. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)
Characterization of new C9orf72 dipeptide repeat antibodies.
Rabbit polyclonal antibodies were generated against the sense strand-derived DPRs GA and GR.(A) Western blot of lysates from COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with the indicated vectors. Blots were serially probed with the indicated DPR antibody, then ab27671 and then actin as a loading control.
All lanes: Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)
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