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AB27671

Anti-V5 tag antibody [SV5-Pk1]

5

(11 Reviews)

|

(205 Publications)

Anti-V5 tag antibody [SV5-Pk1] (ab27671) is a mouse monoclonal antibody detecting V5 tag in Western Blot, Flow Cytometry, IP, IHC-Fr, ELISA.

- Over 150 publications
- Trusted since 2005

View Alternative Names

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, V5 epitope tag

2 Images
Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671)
  • WB

AbReview55266****

Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671)

Cells were transfected with a V5-tagged HSPB8 (MW : ~25kD) plasmid, which expressed a V5-tagged protein with molecular weight of approximately 28kD.

All lanes:

Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution

Lanes 1 - 2:

Transfected NIH/3T3 cell lysate

Lane 3:

Not transfected NIH/3T3 cell lysate

Secondary

All lanes:

HRP-conjugated rabbit polyclonal Anti-mouse

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Exposure time: 1s

Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671)
  • WB

PubMed

Western blot - Anti-V5 tag antibody [SV5-Pk1] (AB27671)

Characterization of new C9orf72 dipeptide repeat antibodies.
Rabbit polyclonal antibodies were generated against the sense strand-derived DPRs GA and GR.

(A) Western blot of lysates from COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with the indicated vectors. Blots were serially probed with the indicated DPR antibody, then ab27671 and then actin as a loading control.

All lanes:

Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)

false

Flores et al PLoS One. 2016 Oct 24;11(10):e0165084. doi: 10.1371/journal.pone.0165084. eCollection 2016. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  • Biotin

    Biotin Anti-V5 tag antibody [SV5-Pk1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SV5-Pk1

Isotype

IgG2a

Carrier free

No

Applications

RIA, WB, IHC-Fr, ELISA, Flow Cyt, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

ab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.

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Reactivity data

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Product details

Anti-V5 tag antibody [SV5-Pk1] (ab27671) is a mouse monoclonal antibody and is validated for use in ELISA, Flow Cyt, IHC-Fr, IP, RIA, WB in human, mouse samples.

Anti-V5 tag antibody [SV5-Pk1] (ab27671) has been cited over 153 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-V5 tag antibody [SV5-Pk1] (ab27671) has high sensitivity and specificity.

Anti-V5 tag antibody [SV5-Pk1] (ab27671) has 11 independent reviews from customers.

Anti-V5 tag antibody [SV5-Pk1] (ab27671) specifically detects V5 tag (UniProt ID: P11207; Molecular weight: 24kDa) and is sold in 100 µg selling sizes.

Antibody clone SV5-Pk1 is also available pre-conjugated to Biotin for your convenience (ab27672).

ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
This antibody was purified from tissue culture supernatant by affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.
Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information V5 tag
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Publications (205)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40523800

2025

No evidence that human GIGYF2 interacts with GRB10: implications for human disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Hyun Choi,Israel Shpilman,Niaz Mahmood,Shaghayegh Farhangmehr,Ulrich Braunschweig,Nathalia Gomide Cruz,Jun Luo,Reese Jalal Ladak,Angelos Pistofidis,Jiannis Ragoussis,T Martin Schmeing,Seyed Mehdi Jafarnejad,Benjamin J Blencowe,Nahum Sonenberg

Nature 644:453-462 PubMed40468081

2025

Molecular gradients shape synaptic specificity of a visuomotor transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Dombrovski,Yixin Zang,Giovanni Frighetto,Andrea Vaccari,HyoJong Jang,Parmis S Mirshahidi,Fangming Xie,Piero Sanfilippo,Bryce W Hina,Aadil Rehan,Roni H Hussein,Pegah S Mirshahidi,Catherine Lee,Aileen Morris,Mark A Frye,Catherine R von Reyn,Yerbol Z Kurmangaliyev,Gwyneth M Card,S Lawrence Zipursky

Nature microbiology 10:1463-1483 PubMed40389650

2025

The fast-evolving FIKK kinase family of Plasmodium falciparum can be inhibited by a single compound.

Applications

Unspecified application

Species

Unspecified reactive species

Hugo Belda,David Bradley,Evangelos Christodoulou,Stephanie D Nofal,Malgorzata Broncel,David Jones,Heledd Davies,M Teresa Bertran,Andrew G Purkiss,Roksana W Ogrodowicz,Dhira Joshi,Nicola O'Reilly,Louise Walport,Andrew Powell,David House,Svend Kjaer,Antoine Claessens,Christian R Landry,Moritz Treeck

Nature communications 16:3401 PubMed40246839

2025

Mitochondrial complexity is regulated at ER-mitochondria contact sites via PDZD8-FKBP8 tethering.

Applications

Unspecified application

Species

Unspecified reactive species

Koki Nakamura,Saeko Aoyama-Ishiwatari,Takahiro Nagao,Mohammadreza Paaran,Christopher J Obara,Yui Sakurai-Saito,Jake Johnston,Yudan Du,Shogo Suga,Masafumi Tsuboi,Makoto Nakakido,Kouhei Tsumoto,Yusuke Kishi,Yukiko Gotoh,Chulhwan Kwak,Hyun-Woo Rhee,Jeong Kon Seo,Hidetaka Kosako,Clint Potter,Bridget Carragher,Jennifer Lippincott-Schwartz,Franck Polleux,Yusuke Hirabayashi

Nature communications 16:2738 PubMed40108168

2025

Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals.

Applications

Unspecified application

Species

Unspecified reactive species

Louis-Mathieu Harvey,Pierre-Marc Frédérick,Rajani Kanth Gudipati,Pascale Michaud,François Houle,Daniel Young,Catherine Desbiens,Shanna Ladouceur,Antoine Dufour,Helge Großhans,Martin J Simard

Journal of virology 99:e0177724 PubMed40067013

2025

BET degraders reveal BRD4 disruption of 7SK and P-TEFb is critical for effective reactivation of latent HIV in CD4+ T-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anne-Marie W Turner,Frances M Bashore,Shane D Falcinelli,Joshua A Fox,Alana L Keller,Anthony D Fenton,Renee F Geyer,Brigitte Allard,Jennifer L Kirchherr,Nancie M Archin,Lindsey I James,David M Margolis

Nature communications 16:1511 PubMed39929834

2025

Loss-of-function mutations in the dystonia gene THAP1 impair proteasome function by inhibiting PSMB5 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Dylan E Ramage,Drew W Grant,Richard T Timms

STAR protocols 6:103617 PubMed39891917

2025

Protocol for assessing the clogging of the mitochondrial translocase of the outer membrane by precursor proteins in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

John Kim,Hilla Weidberg

Nature communications 16:223 PubMed39794322

2025

Girolline is a sequence context-selective modulator of eIF5A activity.

Applications

Unspecified application

Species

Unspecified reactive species

Tilman Schneider-Poetsch,Yongjun Dang,Wakana Iwasaki,Mayumi Arata,Yuichi Shichino,Ali Al Mourabit,Celine Moriou,Daniel Romo,Jun O Liu,Takuhiro Ito,Shintaro Iwasaki,Minoru Yoshida

The Journal of neuroscience : the official journal of the Society for Neuroscience 45: PubMed39779375

2025

Amyloid-β-Driven Synaptic Deficits Are Mediated by Synaptic Removal of GluA3-Containing AMPA Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Niels R Reinders,Sophie J F van der Spek,Remco V Klaassen,Karin J Koymans,Harold D MacGillavry,August B Smit,Helmut W Kessels
View all publications
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