Rabbit Polyclonal Vaccinia Virus antibody. Suitable for ELISA and reacts with Vaccinia virus samples. Cited in 1 publication.
View Alternative Names
Orthopoxvirus
- ELISA
Unknown
ELISA - Anti-Vaccinia Virus antibody (AB26853)
ELISA method :
Antigen is coated on EIA strips at 1μg per well.
Add 200μl blocking buffer then wash wells with PBST.
Antiserum or peptide specific purified antibody is diluted in series as 102~106 folds.
Incubate antibody for 1 hour.
Wash unbound antibodies and add HRP-conjugated anti-rabbit IgG.
Wash plates and add substrate to develop color for 5 minutes.
Read absorbance at 650nm. Amount of colour is directly proportional to amount of antibodies.
Antibody titer is defined as >0.1 of absorbance of antiserum minus pre-immune serum.
Titer ~ 1/437000.
Reactivity data
Properties and storage information
Form
Purity
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Product protocols
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Target data
Publications (1)
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Matrix biology : journal of the International Society for Matrix Biology 112:90-115 PubMed35963565
2022
Applications
Unspecified application
Species
Unspecified reactive species
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