AB235201

Anti-VAChT antibody

Name: Anti-VAChT antibody. Rabbit polyclonal (ab235201) | Abcam
Brand: Abcam Limited
SKU: AB235201

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(9 Publications)

Rabbit Polyclonal VACHT antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Mouse samples. Cited in 9 publications.

View Alternative Names

VACHT, SLC18A3, Vesicular acetylcholine transporter, VAChT, Solute carrier family 18 member 3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody (AB235201)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody (AB235201)

Paraffin-embedded rat spinal cord tissue stained for VAChT using ab235201 at 1/100 dilution for 30 minutes at room temperature. Secondary antibody was an anti-Rabbit Poly-HRP-IgG, ready to use for 8 minutes at room temperature. Counterstained with hematoxylin.

A : Rat spinal cord H&E.

B : ab235201 20X magnification.

C : ab235201 40X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody (AB235201)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody (AB235201)

4% PFA-fixed, 0.3%Triton X-100 permeabilized Post-natal rat pup (PND1) heterogeneous brain cells labeling VAChT
(green) using ab235201 at 15 μg/mL, O/N at 4⁰C. Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5μg/ml for 1 h at RT. The nuclear counterstain was DAPI (blue).

Expected localization : predicted membrane, golgi apparatus

Antibody detecting dendrites and axons and specific organelles in the nucleus (nucleolus). Staining correspondent to confirmed VAChT (Slc18a3) staining in mouse brain P7 Gensat project :

http : //www.gensat.org/imagenavigator.jsp?imageID=66611

(A) DAPI

(B) ab235201 + secondary antibody

(D) Secondary antibody only

Supplier Data

Western blot - Anti-VAChT antibody (AB235201)

All lanes:

Western blot - Anti-VAChT antibody (ab235201) at 1/1000 dilution

All lanes:

Post-natal rat pup whole brain lysate (PND2-6)

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5-15 µg/mL", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Antigen retrieval: HIER using citrate buffer for 20 minutes." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-15 µg/mL", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Antigen retrieval: HIER using citrate buffer for 20 minutes." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The vesicular acetylcholine transporter often referred to as VAChT is responsible for packaging acetylcholine into vesicles in cholinergic neurons. This process is important for synaptic transmission in the nervous system. VAChT functions by using a proton gradient to transport acetylcholine against its concentration gradient into synaptic vesicles. VAChT has a molecular mass of approximately 55 kDa. It is widely expressed in cholinergic neurons throughout the central and peripheral nervous systems particularly in regions such as the cortex hippocampus and neuromuscular junctions.

Biological function summary

The function of VAChT plays an important role in the cholinergic system by facilitating neurotransmitter storage essential for neural communication. As part of the vesicular neurotransmitter transporter family VAChT collectively works with other proteins within this complex system to regulate acetylcholine availability and release. The stored acetylcholine within vesicles is released into the synaptic cleft in response to neuronal signals which promotes synapse firing and communication between neurons.

Pathways

Acetylcholine packaging and release managed by VAChT is integral to the cholinergic signaling pathway. This pathway is fundamental to ensuring proper function of learning memory and muscle control processes. VAChT works closely with the protein choline acetyltransferase (ChAT) which synthesizes acetylcholine from choline and acetyl-CoA. These proteins ensure a seamless conversion and transport loop within cholinergic neurons to maintain efficient neural signaling.

VAChT has been linked to conditions that involve impaired cholinergic function such as Alzheimer's disease and myasthenia gravis. In Alzheimer's disease disruptions in the cholinergic system often marked by reduced VAChT expression contribute to cognitive decline. VAChT variations can also impact acetylcholine availability in myasthenia gravis a disorder characterized by muscle weakness. In both cases the relationship between VAChT and ChAT is important as ChAT levels often change in parallel affecting overall cholinergic signaling and response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electrogenic antiporter that exchanges one cholinergic neurotransmitter, acetylcholine or choline, with two intravesicular protons across the membrane of synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to store neurotransmitters inside the vesicles prior to their release via exocytosis (By similarity) (PubMed : 20225888, PubMed : 8910293). Determines cholinergic vesicular quantal size at presynaptic nerve terminals in developing neuro-muscular junctions with an impact on motor neuron differentiation and innervation pattern (By similarity). Part of forebrain cholinergic system, regulates hippocampal synapse transmissions that underlie spatial memory formation (By similarity). Can transport serotonin.

See full target information SLC18A3

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Biology 14: PubMed41007382

2025

A Simple Three-Dimensional Compartmentalized Co-Culture Model for Basal Forebrain and Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoman Luo,Jing Li,Zhiyu Deng,Yali Xu,Xixi Li,Miao Ren,Xiangning Li

ACS omega 10:30327-30334 PubMed40727794

2025

Early Exposure of an Infantile Rat to Sex-Related Content Induces Precocious Puberty by Activation of Cholinergic Neurons in the Amygdala and KNDy Neurons in the Arcuate Nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Yifen Shen,Tao Yang,Le Zhang,Yanping Hu,Hao Shen,Hua Li,Yi Yang,Shasha Zhou,Yihang Shen

iScience 27:109163 PubMed38425841

2024

Noninvasive neuromodulation protects against doxorubicin-induced cardiotoxicity and inhibits tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjie Xie,Fuding Guo,Lingpeng Song,Wuping Tan,Xinrui Han,Saiting Xu,Xujun Li,Yijun Wang,Yueyi Wang,Liping Zhou,Xiaoya Zhou,Hong Jiang,Lilei Yu

Frontiers in aging neuroscience 14:960314 PubMed36275011

2022

Inhibiting amyloid beta (1-42) peptide-induced mitochondrial dysfunction prevents the degradation of synaptic proteins in the entorhinal cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Olayemi Joseph Olajide,Claudia La Rue,Andreas Bergdahl,Clifton Andrew Chapman

PloS one 17:e0271131 PubMed35939438

2022

Ovariectomy reduces cholinergic modulation of excitatory synaptic transmission in the rat entorhinal cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel A Batallán Burrowes,Olayemi Joseph Olajide,Isabella A Iasenza,Waqqas M Shams,Francis Carter,C Andrew Chapman

Clinical and translational medicine 12:e890 PubMed35758323

2022

Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqiang He,Qi Fu,Min Sun,Yu Qian,Yucheng Liang,Jie Zhang,Rui Gao,Hemin Jiang,Hao Dai,Yuwei Liu,Xinyu Xu,Heng Chen,Kuanfeng Xu,Tao Yang

Frontiers in cell and developmental biology 9:719192 PubMed34497808

2021

PARP1 Inhibitor Combined With Oxaliplatin Efficiently Suppresses Oxaliplatin Resistance in Gastric Cancer-Derived Organoids via Homologous Recombination and the Base Excision Repair Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huafu Li,Chunming Wang,Linxiang Lan,Wenhui Wu,Ian Evans,E Josue Ruiz,Leping Yan,Zhijun Zhou,Joaquim M Oliveira,Rui L Reis,Zhenran Hu,Wei Chen,Axel Behrens,Yulong He,Changhua Zhang

Neuroscience 455:165-176 PubMed33385490

2021

Chronic Ethanol Exposure Potentiates Cholinergic Neurotransmission in the Basolateral Amygdala.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Sizer,Brian C Parrish,Brian A McCool

The American journal of pathology 191:515-526 PubMed33345997

2020

Vascular Endothelial Growth Factor Mediates the Sprouted Axonogenesis of Breast Cancer in Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxiu Han,Chunxue Yang,Yuan Zhang,Changhao Han,Guohua Zhang

View all publications

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Product promise

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Anti-VAChT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody (AB235201)

Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody (AB235201)

Western blot - Anti-VAChT antibody (AB235201)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise