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AB279710

Anti-VAChT antibody [EPR24248-167] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal VACHT antibody. Carrier free. Suitable for IP, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Vacht, Slc18a3, Vesicular acetylcholine transporter, VAChT, Solute carrier family 18 member 3

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)

This data was developed using ab271111, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Punctate staining in mouse cerebrum. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunoprecipitation - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)
  • IP

Supplier Data

Immunoprecipitation - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)

This data was developed using ab271111, the same antibody clone in a different buffer formulation.

VAChT was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab271111 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271111 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 ug

Lane 2 : ab271111 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab271111 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-VAChT antibody [EPR24248-167] (<a href='/en-us/products/primary-antibodies/vacht-antibody-epr24248-167-ab271111'>ab271111</a>)

Predicted band size: 56 kDa

Observed band size: 70 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)

This data was developed using ab271111, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly punctate staining in the corpus striatum of mouse cerebrum. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunoprecipitation - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)
  • IP

Supplier Data

Immunoprecipitation - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)

This data was developed using ab271111, the same antibody clone in a different buffer formulation.

VAChT was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab271111 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271111 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate 10 ug

Lane 2 : ab271111 IP in Rat brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab271111 in rat brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-VAChT antibody [EPR24248-167] (<a href='/en-us/products/primary-antibodies/vacht-antibody-epr24248-167-ab271111'>ab271111</a>)

Predicted band size: 56 kDa

Observed band size: 70 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)

This data was developed using ab271111, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Punctate staining in rat cerebrum. The section was incubated with ab271111 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)

This data was developed using ab271111, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in mouse liver (PMID : 9427309). The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAChT antibody [EPR24248-167] - BSA and Azide free (AB279710)

This data was developed using ab271111, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling VAChT with ab271111 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in rat liver. The section was incubated with ab271111 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24248-167

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

ab279710 is the carrier-free version of ab271111.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The vesicular acetylcholine transporter often referred to as VAChT is responsible for packaging acetylcholine into vesicles in cholinergic neurons. This process is important for synaptic transmission in the nervous system. VAChT functions by using a proton gradient to transport acetylcholine against its concentration gradient into synaptic vesicles. VAChT has a molecular mass of approximately 55 kDa. It is widely expressed in cholinergic neurons throughout the central and peripheral nervous systems particularly in regions such as the cortex hippocampus and neuromuscular junctions.
Biological function summary

The function of VAChT plays an important role in the cholinergic system by facilitating neurotransmitter storage essential for neural communication. As part of the vesicular neurotransmitter transporter family VAChT collectively works with other proteins within this complex system to regulate acetylcholine availability and release. The stored acetylcholine within vesicles is released into the synaptic cleft in response to neuronal signals which promotes synapse firing and communication between neurons.

Pathways

Acetylcholine packaging and release managed by VAChT is integral to the cholinergic signaling pathway. This pathway is fundamental to ensuring proper function of learning memory and muscle control processes. VAChT works closely with the protein choline acetyltransferase (ChAT) which synthesizes acetylcholine from choline and acetyl-CoA. These proteins ensure a seamless conversion and transport loop within cholinergic neurons to maintain efficient neural signaling.

VAChT has been linked to conditions that involve impaired cholinergic function such as Alzheimer's disease and myasthenia gravis. In Alzheimer's disease disruptions in the cholinergic system often marked by reduced VAChT expression contribute to cognitive decline. VAChT variations can also impact acetylcholine availability in myasthenia gravis a disorder characterized by muscle weakness. In both cases the relationship between VAChT and ChAT is important as ChAT levels often change in parallel affecting overall cholinergic signaling and response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Electrogenic antiporter that exchanges one cholinergic neurotransmitter, acetylcholine or choline, with two intravesicular protons across the membrane of synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to store neurotransmitters inside the vesicles prior to their release via exocytosis (By similarity). Determines cholinergic vesicular quantal size at presynaptic nerve terminals in developing neuro-muscular junctions with an impact on motor neuron differentiation and innervation pattern (By similarity) (PubMed : 19635813). Part of forebrain cholinergic system, regulates hippocampal synapse transmissions that underlie spatial memory formation (PubMed : 23045697). Can transport serotonin.
See full target information Slc18a3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurophysiology 127:313-327 PubMed34907797

2021

Efferent neurons control hearing sensitivity and protect hearing from noise through the regulation of gap junctions between cochlear supporting cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Bo Zhao,Li-Man Liu,Ning Yu,Yan Zhu,Ling Mei,Jin Chen,Chun Liang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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