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AB326355

Anti-VAChT antibody [S6]

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Mouse Recombinant Monoclonal VACHT antibody. Suitable for ICC/IF, WB and reacts with Human, Rat samples.

View Alternative Names

VACHT, SLC18A3, Vesicular acetylcholine transporter, VAChT, Solute carrier family 18 member 3, Vacht, Vat, Vesicular acetylcholine transporter, VAChT, rVAT, Solute carrier family 18 member 3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody [S6] (AB326355)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody [S6] (AB326355)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Neuroblastoma cell line (SK-N-BE). Species : Human. Fixation : 4% Formaldehyde for 15 min at RT. Primary Antibody : ab326355 at 1 : 100 for 60 min at RT. Secondary Antibody : Goat Anti-Mouse ATTO 488 at 1 : 200 for 60 min at RT. Counterstain : Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1 : 1000, 1 : 5000 for 60 min at RT, 5 min at RT. Localization : Membrane. Magnification : 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab326355. (D) Composite.

Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody [S6] (AB326355)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAChT antibody [S6] (AB326355)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Neuroblastoma cells (SH-SY5Y). Species : Human. Fixation : 4% PFA for 15 min. Primary Antibody : ab326355 at 1 : 200 for overnight at 4°C with slow rocking. Secondary Antibody : AlexaFluor 488 at 1 : 1000 for 1 hour at RT. Counterstain : Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1 : 800, 1.6mM for 20 min at RT.

Western blot - Anti-VAChT antibody [S6] (AB326355)
  • WB

Supplier Data

Western blot - Anti-VAChT antibody [S6] (AB326355)

Western Blot analysis of Rat brain membrane lysate showing detection of VAChT protein. Primary Antibody : ab326355 at 1 : 1000.

All lanes:

Western blot - Anti-VAChT antibody [S6] (ab326355) at 1/1000 dilution

All lanes:

Rat brain membrane lysate

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

S6

Isotype

IgG1

Carrier free

No

Reacts with

Human, Rat

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Electrogenic antiporter that exchanges one cholinergic neurotransmitter, acetylcholine or choline, with two intravesicular protons across the membrane of synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to store neurotransmitters inside the vesicles prior to their release via exocytosis (By similarity) (PubMed : 20225888, PubMed : 8910293). Determines cholinergic vesicular quantal size at presynaptic nerve terminals in developing neuro-muscular junctions with an impact on motor neuron differentiation and innervation pattern (By similarity). Part of forebrain cholinergic system, regulates hippocampal synapse transmissions that underlie spatial memory formation (By similarity). Can transport serotonin.
See full target information SLC18A3

Additional targets

Slc18a3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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