Rabbit Recombinant Monoclonal valyl tRNA synthetase antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (AB250401), expandable thumbnail](https://content.abcam.com/products/images/valyl-trna-synthetase-antibody-epr11300-bsa-and-azide-free-ab250401--western-blot-img116061.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (AB250401)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (AB250401), expandable thumbnail](https://content.abcam.com/products/images/valyl-trna-synthetase-antibody-epr11300-bsa-and-azide-free-ab250401--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26132.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (AB250401)")
![Immunocytochemistry/ Immunofluorescence - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (AB250401), expandable thumbnail](https://content.abcam.com/products/images/valyl-trna-synthetase-antibody-epr11300-bsa-and-azide-free-ab250401--immunocytochemistry-immunofluorescence-img15390.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (AB250401)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Catalyzes the attachment of valine to tRNA(Val).
Alternative names
G7A, VARS, VARS2, VARS1, Valine--tRNA ligase, Protein G7a, Valyl-tRNA synthetase, ValRS
Recommended products
Rabbit Recombinant Monoclonal valyl tRNA synthetase antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11300
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250401 is the carrier-free version of Anti-valyl tRNA synthetase antibody [EPR11300] - C-terminal ab181175.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (ab250401), expandable thumbnail](https://content.abcam.com/products/images/valyl-trna-synthetase-antibody-epr11300-bsa-and-azide-free-ab250401--western-blot-img116061.jpg "Western blot - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (ab250401)")
Western blot - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (ab250401)
This data was developed using Anti-valyl tRNA synthetase antibody [EPR11300] - C-terminal ab181175, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-valyl tRNA synthetase antibody [EPR11300] - C-terminal (Anti-valyl tRNA synthetase antibody [EPR11300] - C-terminal ab181175) at 1/5000 dilution
Lane 1: HepG2 cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
Lane 3: Ramos cell lysate at 20 µg
Lane 4: K562 cell lysate at 20 µg
SecondaryAll lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 140 kDa
Observed band size: 140 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (ab250401), expandable thumbnail](https://content.abcam.com/products/images/valyl-trna-synthetase-antibody-epr11300-bsa-and-azide-free-ab250401--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26132.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (ab250401)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (ab250401)
This data was developed using Anti-valyl tRNA synthetase antibody [EPR11300] - C-terminal ab181175, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling valyl tRNA synthetase with Anti-valyl tRNA synthetase antibody [EPR11300] - C-terminal ab181175 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (ab250401), expandable thumbnail](https://content.abcam.com/products/images/valyl-trna-synthetase-antibody-epr11300-bsa-and-azide-free-ab250401--immunocytochemistry-immunofluorescence-img15390.jpg "Immunocytochemistry/ Immunofluorescence - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (ab250401)")
Immunocytochemistry/ Immunofluorescence - Anti-valyl tRNA synthetase antibody [EPR11300] - BSA and Azide free (ab250401)
This data was developed using Anti-valyl tRNA synthetase antibody [EPR11300] - C-terminal ab181175, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling valyl tRNA synthetase with Anti-valyl tRNA synthetase antibody [EPR11300] - C-terminal ab181175 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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