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AB180508

Anti-VAM1 antibody [EPR14157(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal VAM1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MPP6, VAM1, PALS2, Protein PALS2, MAGUK p55 subfamily member 6, Veli-associated MAGUK 1, VAM-1

2 Images
Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (AB180508)
  • IP

Supplier Data

Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (AB180508)

Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab180508 at 1/60 dilution.
Secondary : Anti-Rabbit IgG HRP at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (ab180508)

Predicted band size: 61 kDa

false

Western blot - Anti-VAM1 antibody [EPR14157(B)] (AB180508)
  • WB

Supplier Data

Western blot - Anti-VAM1 antibody [EPR14157(B)] (AB180508)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-VAM1 antibody [EPR14157(B)] (ab180508) at 1/5000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

NTERA2/D1 cell lysate at 20 µg

Lane 4:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14157(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAM1 also known as Vacuolar Assembly Machinery Protein 1 is an important component involved in vacuolar membrane fusion and transport processes within cells. The protein has an approximate molecular mass of 55 kDa. It is expressed in various cell types and tissues predominantly within eukaryotic cells where vacuolar functions are critical. In yeast cells VAM1 locates primarily at the vacuolar membrane playing a direct role in vesicle docking and fusion events that are critical for maintaining cellular homeostasis.
Biological function summary

The machinery orchestrated by VAM1 facilitates important vacuolar fusion processes ensuring appropriate cellular trafficking and compartmentalization. VAM1 operates as a part of a larger fusion complex which also includes SNARE proteins and other fusion mediators. Through these interactions VAM1 contributes to the remodeling of vacuolar structures ensuring efficient intracellular transport. Its function affects not just the integrity of vacuolar membranes but also the turnover and degradation of cellular components within the vacuole.

Pathways

VAM1 plays an important role in the homotypic fusion and protein sorting (HOPS) pathway which is important for vacuole docking and fusion. It also influences the endosomal sorting complexes required for transport (ESCRT) pathway where it collaborates with components such as Vps proteins to direct vesicle transport and sorting. These pathways are essential for cellular functions like nutrient recycling and waste degradation processes that significantly impact cell survival and adaptation.

Mutations or dysfunctions in VAM1 can relate to lysosomal storage disorders and neurodegenerative diseases. These conditions can arise due to disruptions in cellular waste processing and protein degradation where VAM1's role in membrane fusion becomes compromised. Furthermore VAM1 interacts with other proteins involved in these processes like the Vps class proteins which also link to various cellular pathologies when their function is impaired. Understanding VAM1 and its network is therefore important for unraveling the molecular basis of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information PALS2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 19:1618-1624 PubMed38051907

2023

Melatonin improves synapse development by PI3K/Akt signaling in a mouse model of autism spectrum disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Luyi Wang,Man Xu,Yan Wang,Feifei Wang,Jing Deng,Xiaoya Wang,Yu Zhao,Ailing Liao,Feng Yang,Shali Wang,Yingbo Li
View all publications
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Product promise

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