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AB250210

Anti-VAM1 antibody [EPR14157(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal VAM1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MPP6, VAM1, PALS2, Protein PALS2, MAGUK p55 subfamily member 6, Veli-associated MAGUK 1, VAM-1

2 Images
Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] - BSA and Azide free (AB250210)
  • IP

Supplier Data

Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] - BSA and Azide free (AB250210)

This data was developed using ab180508, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab180508 at 1/60 dilution.
Secondary : Anti-Rabbit IgG HRP at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (<a href='/en-us/products/primary-antibodies/vam1-antibody-epr14157b-ab180508'>ab180508</a>)

Predicted band size: 61 kDa

false

Western blot - Anti-VAM1 antibody [EPR14157(B)] - BSA and Azide free (AB250210)
  • WB

Supplier Data

Western blot - Anti-VAM1 antibody [EPR14157(B)] - BSA and Azide free (AB250210)

This data was developed using ab180508, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VAM1 antibody [EPR14157(B)] (<a href='/en-us/products/primary-antibodies/vam1-antibody-epr14157b-ab180508'>ab180508</a>) at 1/5000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

NTERA2/D1 cell lysate at 20 µg

Lane 4:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14157(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250210 is the carrier-free version of ab180508.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAM1 also known as Vacuolar Assembly Machinery Protein 1 is an important component involved in vacuolar membrane fusion and transport processes within cells. The protein has an approximate molecular mass of 55 kDa. It is expressed in various cell types and tissues predominantly within eukaryotic cells where vacuolar functions are critical. In yeast cells VAM1 locates primarily at the vacuolar membrane playing a direct role in vesicle docking and fusion events that are critical for maintaining cellular homeostasis.
Biological function summary

The machinery orchestrated by VAM1 facilitates important vacuolar fusion processes ensuring appropriate cellular trafficking and compartmentalization. VAM1 operates as a part of a larger fusion complex which also includes SNARE proteins and other fusion mediators. Through these interactions VAM1 contributes to the remodeling of vacuolar structures ensuring efficient intracellular transport. Its function affects not just the integrity of vacuolar membranes but also the turnover and degradation of cellular components within the vacuole.

Pathways

VAM1 plays an important role in the homotypic fusion and protein sorting (HOPS) pathway which is important for vacuole docking and fusion. It also influences the endosomal sorting complexes required for transport (ESCRT) pathway where it collaborates with components such as Vps proteins to direct vesicle transport and sorting. These pathways are essential for cellular functions like nutrient recycling and waste degradation processes that significantly impact cell survival and adaptation.

Mutations or dysfunctions in VAM1 can relate to lysosomal storage disorders and neurodegenerative diseases. These conditions can arise due to disruptions in cellular waste processing and protein degradation where VAM1's role in membrane fusion becomes compromised. Furthermore VAM1 interacts with other proteins involved in these processes like the Vps class proteins which also link to various cellular pathologies when their function is impaired. Understanding VAM1 and its network is therefore important for unraveling the molecular basis of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information PALS2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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