AB41324

Anti-VAMP1 antibody

(1 Review)

(3 Publications)

Rabbit Polyclonal Synaptobrevin antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

Syb1, Vesicle-associated membrane protein 1, VAMP-1, Synaptobrevin-1

1 Images

Project

Western blot - Anti-VAMP1 antibody (AB41324)

All lanes:

Western blot - Anti-VAMP1 antibody (ab41324) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Lane 3:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 4:

Spinal Cord (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 13 kDa

Observed band size: 17 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 mg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAMP1 also known as Vesicle-Associated Membrane Protein 1 or Synaptobrevin 1 functions as an important component in vesicular transport mechanisms. It is a SNARE protein with a molecular mass of approximately 13 kDa. VAMP1 is widely expressed in neurons particularly in regions related to synaptic activities. It is part of a larger family of proteins that mediate vesicle fusion assisting in neurotransmitter release at synapses by participating in the docking and fusion of vesicles with target membranes.

Biological function summary

VAMP1 plays a role in neurotransmission processes by forming part of the SNARE complex which regulates membrane fusion events. This complex typically includes syntaxin and SNAP-25 acting synergistically to allow neurotransmitter-containing vesicles to fuse with the presynaptic membrane. The involvement of VAMP1 in neurotransmitter release emphasizes its significance in maintaining synaptic function heavily influencing both synaptic plasticity and signal transmission within the nervous system.

Pathways

VAMP1 operates predominantly in the synaptic vesicular transport pathway serving a critical role in exocytosis. It is linked closely with the synaptic vesicle cycle a fundamental pathway that underpins neurotransmitter release in response to an action potential. Proteins such as SNAP-25 and syntaxin-1A accompany VAMP1 in these pathways collectively ensuring efficient synaptic vesicle fusion and recycling which is integral for continuous synaptic transmission.

VAMP1 associations include motor neuron disease (MND) and certain forms of congenital myasthenic syndrome (CMS). Defects in VAMP1 often disrupt synaptic transmission contributing to neuromuscular dysfunctions observed in these conditions. VAMP1 interacts with proteins such as synaptotagmin and complexins which are important for the proper release of neurotransmitters linking VAMP1 dysfunction to impaired synaptic transmission and muscle control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

See full target information Vamp1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastroenterology and hepatology 37:154-163 PubMed34734434

2021

CD155/TIGIT signaling regulates the effector function of tumor-infiltrating CD8+ T cell by NF-κB pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Li,Jihu Ding,Ying Wang,Xianling Wang,Lin Lv

Cell systems 12:141-158.e9 PubMed33326751

2020

Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy J Hark,Nalini R Rao,Charlotte Castillon,Tamara Basta,Samuel Smukowski,Huan Bao,Arun Upadhyay,Ewa Bomba-Warczak,Toshihiro Nomura,Eileen T O'Toole,Garry P Morgan,Laith Ali,Takashi Saito,Christelle Guillermier,Takaomi C Saido,Matthew L Steinhauser,Michael H B Stowell,Edwin R Chapman,Anis Contractor,Jeffrey N Savas

Progress in neuro-psychopharmacology & biological 95:109669 PubMed31228641

2019

Differential expression of synaptic markers regulated during neurodevelopment in a rat model of schizophrenia-like behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Betina Elfving,Heidi Kaastrup Müller,Ignasi Oliveras,Tina Becher Østerbøg,Cristobal Rio-Alamos,Ana Sanchez-Gonzalez,Adolf Tobeña,Alberto Fernandez-Teruel,Susana Aznar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VAMP1 antibody

Western blot - Anti-VAMP1 antibody (AB41324)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise