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Rabbit Recombinant Monoclonal Synaptobrevin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.


Images

Western blot - Anti-VAMP1 antibody [EPR7325(2)] (AB151712), expandable thumbnail
  • Western blot - Anti-VAMP1 antibody [EPR7325(2)] (AB151712), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Mouse
Expected
Not recommended
Tested
Not recommended
Rat
Expected
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

Alternative names

SYB1, VAMP1, Vesicle-associated membrane protein 1, VAMP-1, Synaptobrevin-1

Recommended products

Rabbit Recombinant Monoclonal Synaptobrevin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7325(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

VAMP1 also known as Vesicle-Associated Membrane Protein 1 or Synaptobrevin 1 functions as an important component in vesicular transport mechanisms. It is a SNARE protein with a molecular mass of approximately 13 kDa. VAMP1 is widely expressed in neurons particularly in regions related to synaptic activities. It is part of a larger family of proteins that mediate vesicle fusion assisting in neurotransmitter release at synapses by participating in the docking and fusion of vesicles with target membranes.

Biological function summary

VAMP1 plays a role in neurotransmission processes by forming part of the SNARE complex which regulates membrane fusion events. This complex typically includes syntaxin and SNAP-25 acting synergistically to allow neurotransmitter-containing vesicles to fuse with the presynaptic membrane. The involvement of VAMP1 in neurotransmitter release emphasizes its significance in maintaining synaptic function heavily influencing both synaptic plasticity and signal transmission within the nervous system.

Pathways

VAMP1 operates predominantly in the synaptic vesicular transport pathway serving a critical role in exocytosis. It is linked closely with the synaptic vesicle cycle a fundamental pathway that underpins neurotransmitter release in response to an action potential. Proteins such as SNAP-25 and syntaxin-1A accompany VAMP1 in these pathways collectively ensuring efficient synaptic vesicle fusion and recycling which is integral for continuous synaptic transmission.

Associated diseases and disorders

VAMP1 associations include motor neuron disease (MND) and certain forms of congenital myasthenic syndrome (CMS). Defects in VAMP1 often disrupt synaptic transmission contributing to neuromuscular dysfunctions observed in these conditions. VAMP1 interacts with proteins such as synaptotagmin and complexins which are important for the proper release of neurotransmitters linking VAMP1 dysfunction to impaired synaptic transmission and muscle control.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712), expandable thumbnail

    Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds

    All lanes: Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa

    Observed band size: 16 kDa

  • Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712), expandable thumbnail

    Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    All lanes: Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712) at 1/1000 dilution

    All lanes: Human cerebellum lysate at 10 µg

    Predicted band size: 13 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab151712 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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