AB151712

Anti-VAMP1 antibody [EPR7325(2)]

RabMAb
Recombinant
What is this?

5

(1 Review)

|

(4 Publications)

Rabbit Recombinant Monoclonal Synaptobrevin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

SYB1, VAMP1, Vesicle-associated membrane protein 1, VAMP-1, Synaptobrevin-1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab151712 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151712 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151712 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab151712 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab151712 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

WB

Unknown

Western blot - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

All lanes:

Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712) at 1/1000 dilution

All lanes:

Human cerebellum lysate at 10 µg

Predicted band size: 13 kDa

false

Western blot - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

WB

Supplier Data

Western blot - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 10 seconds

All lanes:

Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

Observed band size: 16 kDa

false

Carrier free

Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7325(2)

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse

Applications

IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAMP1 also known as Vesicle-Associated Membrane Protein 1 or Synaptobrevin 1 functions as an important component in vesicular transport mechanisms. It is a SNARE protein with a molecular mass of approximately 13 kDa. VAMP1 is widely expressed in neurons particularly in regions related to synaptic activities. It is part of a larger family of proteins that mediate vesicle fusion assisting in neurotransmitter release at synapses by participating in the docking and fusion of vesicles with target membranes.

Biological function summary

VAMP1 plays a role in neurotransmission processes by forming part of the SNARE complex which regulates membrane fusion events. This complex typically includes syntaxin and SNAP-25 acting synergistically to allow neurotransmitter-containing vesicles to fuse with the presynaptic membrane. The involvement of VAMP1 in neurotransmitter release emphasizes its significance in maintaining synaptic function heavily influencing both synaptic plasticity and signal transmission within the nervous system.

Pathways

VAMP1 operates predominantly in the synaptic vesicular transport pathway serving a critical role in exocytosis. It is linked closely with the synaptic vesicle cycle a fundamental pathway that underpins neurotransmitter release in response to an action potential. Proteins such as SNAP-25 and syntaxin-1A accompany VAMP1 in these pathways collectively ensuring efficient synaptic vesicle fusion and recycling which is integral for continuous synaptic transmission.

VAMP1 associations include motor neuron disease (MND) and certain forms of congenital myasthenic syndrome (CMS). Defects in VAMP1 often disrupt synaptic transmission contributing to neuromuscular dysfunctions observed in these conditions. VAMP1 interacts with proteins such as synaptotagmin and complexins which are important for the proper release of neurotransmitters linking VAMP1 dysfunction to impaired synaptic transmission and muscle control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

See full target information VAMP1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 16:915969 PubMed35669106

2022

Ablation of GSDMD Attenuates Neurological Deficits and Neuropathological Alterations After Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Du,Chang-Hong Li,Ruo-Bing Gao,Xiao-Qing Cen,Ping Li

Neural plasticity 2022:1353778 PubMed35494481

2022

Dexmedetomidine Mitigates Microglial Activation Associated with Postoperative Cognitive Dysfunction by Modulating the MicroRNA-103a-3p/VAMP1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhichao Wu,Han Wang,Zuan Shi,Yalan Li

Oxidative medicine and cellular longevity 2021:3298400 PubMed34777683

2021

Trilobatin Alleviates Cognitive Deficits and Pathologies in an Alzheimer's Disease Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Jiuyang Ding,Jian Huang,Dan Yin,Ting Liu,Zheng Ren,Shanshan Hu,Yuanliang Ye,Cuiyun Le,Na Zhao,Hongmei Zhou,Zhu Li,Xiaolan Qi,Jiang Huang

Theranostics 7:2452-2462 PubMed28744327

2017

miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease.

Applications

WB

Species

Mouse

Lu-Shiun Her,Su-Han Mao,Chih-Yi Chang,Pei-Hsun Cheng,Yu-Fan Chang,Han-In Yang,Chuan-Mu Chen,Shang-Hsun Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com