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Rabbit Recombinant Monoclonal Synaptobrevin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (AB249031), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (AB249031), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (AB249031), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (AB249031), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (AB249031), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Mouse
Expected
Not recommended
Tested
Not recommended
Rat
Expected
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

Alternative names

SYB1, VAMP1, Vesicle-associated membrane protein 1, VAMP-1, Synaptobrevin-1

Recommended products

Rabbit Recombinant Monoclonal Synaptobrevin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7325(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249031 is the carrier-free version of Anti-VAMP1 antibody [EPR7325(2)] ab151712.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

VAMP1 also known as Vesicle-Associated Membrane Protein 1 or Synaptobrevin 1 functions as an important component in vesicular transport mechanisms. It is a SNARE protein with a molecular mass of approximately 13 kDa. VAMP1 is widely expressed in neurons particularly in regions related to synaptic activities. It is part of a larger family of proteins that mediate vesicle fusion assisting in neurotransmitter release at synapses by participating in the docking and fusion of vesicles with target membranes.

Biological function summary

VAMP1 plays a role in neurotransmission processes by forming part of the SNARE complex which regulates membrane fusion events. This complex typically includes syntaxin and SNAP-25 acting synergistically to allow neurotransmitter-containing vesicles to fuse with the presynaptic membrane. The involvement of VAMP1 in neurotransmitter release emphasizes its significance in maintaining synaptic function heavily influencing both synaptic plasticity and signal transmission within the nervous system.

Pathways

VAMP1 operates predominantly in the synaptic vesicular transport pathway serving a critical role in exocytosis. It is linked closely with the synaptic vesicle cycle a fundamental pathway that underpins neurotransmitter release in response to an action potential. Proteins such as SNAP-25 and syntaxin-1A accompany VAMP1 in these pathways collectively ensuring efficient synaptic vesicle fusion and recycling which is integral for continuous synaptic transmission.

Associated diseases and disorders

VAMP1 associations include motor neuron disease (MND) and certain forms of congenital myasthenic syndrome (CMS). Defects in VAMP1 often disrupt synaptic transmission contributing to neuromuscular dysfunctions observed in these conditions. VAMP1 interacts with proteins such as synaptotagmin and complexins which are important for the proper release of neurotransmitters linking VAMP1 dysfunction to impaired synaptic transmission and muscle control.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031), expandable thumbnail

    Western blot - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031)

    This data was developed using Anti-VAMP1 antibody [EPR7325(2)] ab151712, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-VAMP1 antibody [EPR7325(2)] (Anti-VAMP1 antibody [EPR7325(2)] ab151712) at 1/1000 dilution

    All lanes: Human cerebellum lysate at 10 µg

    Predicted band size: 13 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031)

    This data was developed using Anti-VAMP1 antibody [EPR7325(2)] ab151712, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human brain tissue using Anti-VAMP1 antibody [EPR7325(2)] ab151712 showing +ve staining. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031)

    This data was developed using Anti-VAMP1 antibody [EPR7325(2)] ab151712, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human colon tissue using Anti-VAMP1 antibody [EPR7325(2)] ab151712 showing -ve staining. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031)

    This data was developed using Anti-VAMP1 antibody [EPR7325(2)] ab151712, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human liver tissue using Anti-VAMP1 antibody [EPR7325(2)] ab151712 showing -ve staining. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031)

    This data was developed using Anti-VAMP1 antibody [EPR7325(2)] ab151712, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using Anti-VAMP1 antibody [EPR7325(2)] ab151712 showing -ve staining. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] - BSA and Azide free (ab249031)

    This data was developed using Anti-VAMP1 antibody [EPR7325(2)] ab151712, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using Anti-VAMP1 antibody [EPR7325(2)] ab151712 showing -ve staining. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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