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Rabbit Multiclonal Synaptobrevin antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human VAMP1.

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Images

Western blot - Anti-VAMP1 antibody [RP23040244] (AB308137), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-VAMP1 antibody [RP23040244] (AB308137), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • Synthetic Peptide within Human VAMP1. Database link P23763

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Mouse
Tested
Expected
Rat
Tested
Tested

Tested
Tested

Species

Rat

Dilution info

2.5 µg/mL

Notes

-

Species

Mouse

Dilution info

2.5 µg/mL

Notes

-

Tested
Tested

Species

Rat

Dilution info

5 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

Alternative names

Recommended products

Rabbit Multiclonal Synaptobrevin antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human VAMP1.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogen
  • Synthetic Peptide within Human VAMP1. Database link P23763
Clone number

RP23040244

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

VAMP1 also known as Vesicle-Associated Membrane Protein 1 or Synaptobrevin 1 functions as an important component in vesicular transport mechanisms. It is a SNARE protein with a molecular mass of approximately 13 kDa. VAMP1 is widely expressed in neurons particularly in regions related to synaptic activities. It is part of a larger family of proteins that mediate vesicle fusion assisting in neurotransmitter release at synapses by participating in the docking and fusion of vesicles with target membranes.

Biological function summary

VAMP1 plays a role in neurotransmission processes by forming part of the SNARE complex which regulates membrane fusion events. This complex typically includes syntaxin and SNAP-25 acting synergistically to allow neurotransmitter-containing vesicles to fuse with the presynaptic membrane. The involvement of VAMP1 in neurotransmitter release emphasizes its significance in maintaining synaptic function heavily influencing both synaptic plasticity and signal transmission within the nervous system.

Pathways

VAMP1 operates predominantly in the synaptic vesicular transport pathway serving a critical role in exocytosis. It is linked closely with the synaptic vesicle cycle a fundamental pathway that underpins neurotransmitter release in response to an action potential. Proteins such as SNAP-25 and syntaxin-1A accompany VAMP1 in these pathways collectively ensuring efficient synaptic vesicle fusion and recycling which is integral for continuous synaptic transmission.

Associated diseases and disorders

VAMP1 associations include motor neuron disease (MND) and certain forms of congenital myasthenic syndrome (CMS). Defects in VAMP1 often disrupt synaptic transmission contributing to neuromuscular dysfunctions observed in these conditions. VAMP1 interacts with proteins such as synaptotagmin and complexins which are important for the proper release of neurotransmitters linking VAMP1 dysfunction to impaired synaptic transmission and muscle control.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Western blot - Anti-VAMP1 antibody [RP23040244] (ab308137), expandable thumbnail

    Western blot - Anti-VAMP1 antibody [RP23040244] (ab308137)

    Western blot analysis was performed on Whole cell extracts (30 µg lysate) of PC-12 (Lane 1), PC-12 treated with NGF and KCl (NGF at 200 nM, KCl at 50 nM for 7 days) (Lane 2) and tissue extracts (30 µg lysate) of Mouse Brain (Lane 3) and Rat Brain (Lane 4). The blots were probed with Anti-VAMP1 Recombinant Rabbit Polyclonal Antibody (ab308137, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1:4000 dilution). A 16 kDa band corresponding to VAMP1 was observed specifically in NGF differentiated PC-12 and tissues tested. Known quantity of protein samples were electrophoresed using a 10% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

    Lane 1: PC-12 whole cell extracts without treatment at 30 µg

    Lane 2: PC-12 whole cell extracts treated with NGF and KCl (NGF at 200 nM, KCl at 50 nM for 7 days) at 30 µg

    Lane 3: Mouse Brain tissue lysate at 30 µg

    Lane 4: Rat Brain tissue lysate at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

    Developed using the ECL technique.

  • Immunocytochemistry/ Immunofluorescence - Anti-VAMP1 antibody [RP23040244] (ab308137), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-VAMP1 antibody [RP23040244] (ab308137)

    Immunofluorescence analysis of PC12 cells labeling endogenous VAMP1 with ab308137 at 5 µg/mL, followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at 1:2000 (Green).
    Nuclei (blue) is stained using DAPI.
    Cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (1:300).
    Panels a-d show representative undifferentiated cells that were stained for detection and localization of VAMP1 protein (green) with no signal.
    Panels e-h clearly demonstrate enhanced signal of VAMP1 in cell body and neuronal processes of NGF (200 ng/mL 5 days) differentiated PC12 cells.
    The images were captured at 60X magnification.

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Product protocols

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