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Mouse Monoclonal VAMP2 antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Rat Vamp2.

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Images

Western blot - Anti-VAMP2 antibody [6F9] - N-terminal (AB181754), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [6F9] - N-terminal (AB181754), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Rat Vamp2. The exact immunogen used to generate this antibody is proprietary information. Database link P63045

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Rat
Tested
Tested

Tested
Tested

Species

Rat

Dilution info

1 µg/mL

Notes

-

Tested
Tested

Species

Rat

Dilution info

10 µg/mL

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles (Probable). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:19690160).

Alternative names

Vesicle-associated membrane protein 2, VAMP-2, Synaptobrevin-2, Syb2, Vamp2

Recommended products

Mouse Monoclonal VAMP2 antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Rat Vamp2.

Alternative names

Vesicle-associated membrane protein 2, VAMP-2, Synaptobrevin-2, Syb2, Vamp2

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Rat Vamp2. The exact immunogen used to generate this antibody is proprietary information. Database link P63045
Clone number

6F9

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Vesicle-associated membrane protein 2 (VAMP2) also known as synaptobrevin 2 is a member of the SNARE protein family with a molecular mass of approximately 13 kDa. VAMP2 plays an important role in vesicle trafficking and membrane fusion particularly in the nervous system. It is prominently expressed in the brain where it facilitates neurotransmitter release by forming SNARE complexes essential for synaptic vesicle fusion with the presynaptic membrane.

Biological function summary

VAMP2 interacts with other SNARE proteins such as synaptosomal-associated protein 25 (SNAP-25) and syntaxin-1 to form stable complexes necessary for synaptic vesicle docking and exocytosis. As part of the SNARE complex VAMP2 contributes to the precise regulation of neurotransmitter release in neurons which is critical for synaptic transmission and plasticity. Its function is vital for the communication between neurons and proper brain function.

Pathways

VAMP2 plays a significant role in the synaptic vesicle cycle and neurotransmitter release pathways. It is central to the exocytosis pathway where it interacts with Rab3a a small GTPase that regulates vesicle docking. By participating in these pathways VAMP2 ensures efficient neurotransmission and contributes to neuronal communication processes. VAMP2's interaction with related proteins like SNAP-25 and syntaxin-1 highlights its importance in these synaptic processes.

Associated diseases and disorders

VAMP2 has associations with conditions such as epilepsy and neurodegenerative diseases. Alterations in VAMP2 expression or function can disrupt normal neurotransmitter release impacting synaptic function and leading to neurological symptoms. VAMP2's relationship with SNAP-25 and syntaxin-1 further implicates it in these disorders as these proteins collectively regulate neural communication. Understanding VAMP2's role in these illnesses aids in the development of potential therapeutic strategies targeting SNARE complex dysfunctions.

Product promise

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2 product images

  • Western blot - Anti-VAMP2 antibody [6F9] - N-terminal (ab181754), expandable thumbnail

    Western blot - Anti-VAMP2 antibody [6F9] - N-terminal (ab181754)

    All lanes: Western blot - Anti-VAMP2 antibody [6F9] - N-terminal (ab181754) at 1 µg/mL

    Lane 1: Rat brain membranes, untreated

    Lane 2: Rat brain membranes treated with botulinum neurotoxin A

    Predicted band size: 13 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [6F9] - N-terminal (ab181754), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [6F9] - N-terminal (ab181754)

    Immunocytochemical staining of rat hippocampal neurons stained with ab181754 at 10 μg/ml.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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