AB181754

Anti-VAMP2 antibody [6F9] - N-terminal

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(2 Publications)

Mouse Monoclonal VAMP2 antibody 6F9. N-terminal Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 2 publications.

View Alternative Names

Syb2, Vamp2, Vesicle-associated membrane protein 2, VAMP-2, Synaptobrevin-2

2 Images

Supplier Data

Western blot - Anti-VAMP2 antibody [6F9] - N-terminal (AB181754)

All lanes:

Western blot - Anti-VAMP2 antibody [6F9] - N-terminal (ab181754) at 1 µg/mL

Lane 1:

Rat brain membranes, untreated

Lane 2:

Rat brain membranes treated with botulinum neurotoxin A

Predicted band size: 13 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [6F9] - N-terminal (AB181754)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [6F9] - N-terminal (AB181754)

Immunocytochemical staining of rat hippocampal neurons stained with ab181754 at 10 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6F9

Isotype

IgG1

Carrier free

Reacts with

Rat

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Rat Vamp2. The exact immunogen used to generate this antibody is proprietary information.

P63045

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 99% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vesicle-associated membrane protein 2 (VAMP2) also known as synaptobrevin 2 is a member of the SNARE protein family with a molecular mass of approximately 13 kDa. VAMP2 plays an important role in vesicle trafficking and membrane fusion particularly in the nervous system. It is prominently expressed in the brain where it facilitates neurotransmitter release by forming SNARE complexes essential for synaptic vesicle fusion with the presynaptic membrane.

Biological function summary

VAMP2 interacts with other SNARE proteins such as synaptosomal-associated protein 25 (SNAP-25) and syntaxin-1 to form stable complexes necessary for synaptic vesicle docking and exocytosis. As part of the SNARE complex VAMP2 contributes to the precise regulation of neurotransmitter release in neurons which is critical for synaptic transmission and plasticity. Its function is vital for the communication between neurons and proper brain function.

Pathways

VAMP2 plays a significant role in the synaptic vesicle cycle and neurotransmitter release pathways. It is central to the exocytosis pathway where it interacts with Rab3a a small GTPase that regulates vesicle docking. By participating in these pathways VAMP2 ensures efficient neurotransmission and contributes to neuronal communication processes. VAMP2's interaction with related proteins like SNAP-25 and syntaxin-1 highlights its importance in these synaptic processes.

VAMP2 has associations with conditions such as epilepsy and neurodegenerative diseases. Alterations in VAMP2 expression or function can disrupt normal neurotransmitter release impacting synaptic function and leading to neurological symptoms. VAMP2's relationship with SNAP-25 and syntaxin-1 further implicates it in these disorders as these proteins collectively regulate neural communication. Understanding VAMP2's role in these illnesses aids in the development of potential therapeutic strategies targeting SNARE complex dysfunctions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles (Probable). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed : 19690160).

See full target information Vamp2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Reproduction (Cambridge, England) 160:533-546 PubMed32698156

2020

Membrane trafficking directed by VAMP2 and syntaxin 3 in uterine epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sadaf N Kalam,Louise Cole,Laura Lindsay,Christopher R Murphy

Proceedings of the National Academy of Sciences of 116:13621-13630 PubMed31196958

2019

Region-specific and activity-dependent regulation of SVZ neurogenesis and recovery after stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Huixuan Liang,Handi Zhao,Amy Gleichman,Michal Machnicki,Sagar Telang,Sydney Tang,Mary Rshtouni,Jack Ruddell,S Thomas Carmichael

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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