JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181869

Anti-VAMP2 antibody [EPR12790]

5

(1 Review)

|

(20 Publications)

Anti-VAMP2 antibody [EPR12790] (ab181869) is a rabbit monoclonal antibody detecting VAMP2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

SYB2, VAMP2, Vesicle-associated membrane protein 2, VAMP-2, Synaptobrevin-2

12 Images
Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (AB181869)

Immunofluorescent analysis of SH-SYSY cells (human cell line) (4% paraformaldehyde-fixed) labeling VAMP2 with unpurified ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with DAPI.

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (AB181869)

Immunofluorescent analysis of U87-MG cells (4% paraformaldehyde-fixed) labeling VAMP2 with ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with Dapi.

Flow Cytometry (Intracellular) - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-VAMP2 antibody [EPR12790] (AB181869)

Intracellular Flow Cytometry analysis of SH-SY5Y cells labelling VAMP2 with purified ab181869 at 1/100 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Flow Cytometry (Intracellular) - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-VAMP2 antibody [EPR12790] (AB181869)

Intracellular Flow Cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde labeling VAMP2 with unpurified ab181869 at 1/150 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (AB181869)

Immunocytochemistry/Immunofluorescence analysis of U87-MG (human glioblastoma) cells labelling VAMP2 with purified ab181869 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (AB181869)

ab181869 staining VAMP2 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Negative control 1 : PBS only.

Western blot - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • WB

Unknown

Western blot - Anti-VAMP2 antibody [EPR12790] (AB181869)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution

All lanes:

Human cerebellum lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 13 kDa

false

Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • IP

Supplier Data

Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (AB181869)

Western blot analysis of immunoprecipitation pellet from Human cerebellum lysate immunoprecipitated using ab181869 at 1/70 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (ab181869)

Predicted band size: 13 kDa

false

Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • IP

Unknown

Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (AB181869)

ab181869 (purified) at 1/150 immunoprecipitating VAMP2 in Human cerebellum whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (ab181869)

Predicted band size: 13 kDa

false

Western blot - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • WB

Supplier Data

Western blot - Anti-VAMP2 antibody [EPR12790] (AB181869)

All lanes:

Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human cerebellum lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 13 kDa

false

Western blot - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • WB

Unknown

Western blot - Anti-VAMP2 antibody [EPR12790] (AB181869)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/10000 dilution

All lanes:

Rat heart lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 13 kDa

false

Western blot - Anti-VAMP2 antibody [EPR12790] (AB181869)
  • WB

Unknown

Western blot - Anti-VAMP2 antibody [EPR12790] (AB181869)

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution

All lanes:

Mouse brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa,36 kDa

false

  • HRP

    HRP Anti-VAMP2 antibody [EPR12790]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-VAMP2 antibody [EPR12790]

  • 578 PE

    PE Anti-VAMP2 antibody [EPR12790]

  • Carrier free

    Anti-VAMP2 antibody [EPR12790] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12790

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/150", "IP-species-notes": "<p><strong>For unpurified use at 1/70.</strong></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/500.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p>For unpurified use at 1/150. <a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

What is this antibody validated in?
Anti-VAMP2 antibody [EPR12790] (ab181869) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of VAMP2?
Anti-VAMP2 [EPR12790] (ab181869) specifically detects a band for VAMP2 (UniProt: P63027) at a molecular weight of 13kDa.

Trusted by the scientific community
Anti-VAMP2 [EPR12790] (ab181869) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR12790] also available for your convenience: ab181869, HRP - ab198948, Alexa Fluor® 647 - ab198949, PE - ab214529, Carrier free - ab214590

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vesicle-associated membrane protein 2 (VAMP2) also known as synaptobrevin 2 is a member of the SNARE protein family with a molecular mass of approximately 13 kDa. VAMP2 plays an important role in vesicle trafficking and membrane fusion particularly in the nervous system. It is prominently expressed in the brain where it facilitates neurotransmitter release by forming SNARE complexes essential for synaptic vesicle fusion with the presynaptic membrane.
Biological function summary

VAMP2 interacts with other SNARE proteins such as synaptosomal-associated protein 25 (SNAP-25) and syntaxin-1 to form stable complexes necessary for synaptic vesicle docking and exocytosis. As part of the SNARE complex VAMP2 contributes to the precise regulation of neurotransmitter release in neurons which is critical for synaptic transmission and plasticity. Its function is vital for the communication between neurons and proper brain function.

Pathways

VAMP2 plays a significant role in the synaptic vesicle cycle and neurotransmitter release pathways. It is central to the exocytosis pathway where it interacts with Rab3a a small GTPase that regulates vesicle docking. By participating in these pathways VAMP2 ensures efficient neurotransmission and contributes to neuronal communication processes. VAMP2's interaction with related proteins like SNAP-25 and syntaxin-1 highlights its importance in these synaptic processes.

VAMP2 has associations with conditions such as epilepsy and neurodegenerative diseases. Alterations in VAMP2 expression or function can disrupt normal neurotransmitter release impacting synaptic function and leading to neurological symptoms. VAMP2's relationship with SNAP-25 and syntaxin-1 further implicates it in these disorders as these proteins collectively regulate neural communication. Understanding VAMP2's role in these illnesses aids in the development of potential therapeutic strategies targeting SNARE complex dysfunctions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles (By similarity) (PubMed : 30929742). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.
See full target information VAMP2
style
left-column

Publications (20)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39456743

2024

Constitutive Pleiotrophin Deletion Results in a Phenotype with an Altered Pancreatic Morphology and Function in Old Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Ballesteros-Pla,Julio Sevillano,María Gracia Sánchez-Alonso,María Limones,Jimena Pita,Begoña Zapatería,Marta Inmaculada Sanz-Cuadrado,Javier Pizarro-Delgado,Adriana Izquierdo-Lahuerta,Gema Medina-Gómez,Gonzalo Herradón,María Del Pilar Ramos-Álvarez

iScience 27:110131 PubMed38957789

2024

An HIV-1 CRISPR-Cas9 membrane trafficking screen reveals a role for PICALM intersecting endolysosomes and immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Guizar,Ana Luiza Abdalla,Anne Monette,Kristin Davis,Ramon Edwin Caballero,Meijuan Niu,Xinyun Liu,Oluwaseun Ajibola,Thomas T Murooka,Chen Liang,Andrew J Mouland

CNS neuroscience & therapeutics 30:e14613 PubMed38379185

2024

Targeting blood brain barrier-Remote ischemic conditioning alleviates cognitive impairment in female APP/PS1 rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxuan Ma,Wuxiang Sun,Jing Bai,Fujia Gao,Haoran Ma,Huiyu Liu,Jiewei Hu,Chao Xu,Xin Zhang,Zixuan Liu,Tao Yuan,Chenxu Sun,Yuanyuan Huang,Ruimin Wang

Journal of cellular biochemistry 125:e30529 PubMed38308620

2024

Palmitoylation is required for Sept8-204 and Sept5 to form vesicle-like structure and colocalize with synaptophysin.

Applications

Unspecified application

Species

Unspecified reactive species

Huicong Liu,Rong Tan,Jia Tong,Shuo Wen,Can Wu,Muding Rao,Jiangli Zhu,Shiqian Qi,Eryan Kong

Nature communications 14:5113 PubMed37607933

2023

SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility.

Applications

Unspecified application

Species

Unspecified reactive species

Jiali Liu,Louis Hermo,Deqiang Ding,Chao Wei,Jeffrey M Mann,Xiaoyuan Yan,Ashley F Melnick,Yingjie Wu,Alicia Withrow,Jose Cibelli,Rex A Hess,Chen Chen

The Journal of cell biology 221: PubMed36053215

2022

VAMP4 regulates insulin levels by targeting secretory granules to lysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Fengping Feng,Han Feng,Pengkai Hu,Yanhong Xue,Tao Xu,Eli Song

Nature communications 12:6537 PubMed34764278

2021

Insulin-activated store-operated Ca entry via Orai1 induces podocyte actin remodeling and causes proteinuria.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hee Kim,Kyu-Hee Hwang,Bao T N Dang,Minseob Eom,In Deok Kong,Yousang Gwack,Seyoung Yu,Heon Yung Gee,Lutz Birnbaumer,Kyu-Sang Park,Seung-Kuy Cha

Diabetes 71:275-284 PubMed34753799

2021

Human Islet MicroRNA-200c Is Elevated in Type 2 Diabetes and Targets the Transcription Factor ETV5 to Reduce Insulin Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Jones K Ofori,Alexandros Karagiannopoulos,Mototsugu Nagao,Efraim Westholm,Shaima Ramadan,Anna Wendt,Jonathan L S Esguerra,Lena Eliasson

Brain : a journal of neurology 144:1661-1669 PubMed33760024

2021

CSPα reduces aggregates and rescues striatal dopamine release in α-synuclein transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Caló,Eric Hidari,Michal Wegrzynowicz,Jeffrey W Dalley,Bernard L Schneider,Martyna Podgajna,Oleg Anichtchik,Emma Carlson,David Klenerman,Maria Grazia Spillantini

Genes 12: PubMed33801790

2021

Reduced Interaction of Aggregated α-Synuclein and VAMP2 by Environmental Enrichment Alleviates Hyperactivity and Anxiety in a Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kyungri Kim,Soohyun Wi,Jung Hwa Seo,Soonil Pyo,Sung-Rae Cho
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB228996

Anti-VAMP2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody (AB228996)

  • 0
  • 0
Primary Antibodies

AB3347

Anti-VAMP2 antibody

Western blot - Anti-VAMP2 antibody (AB3347)

  • 4
  • 3
Primary Antibodies

AB181754

Anti-VAMP2 antibody [6F9] - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [6F9] - N-terminal (AB181754)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB108990

Anti-SNAP25 antibody [EP3274]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] (AB108990)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32127

Anti-Synaptophysin antibody [YE269] - Synaptic Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB32127)

  • 5
  • 37

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com