Name: Anti-VAMP2 antibody [EPR12790]
SKU: ab181869
Rating: 5 (1 reviews)

Rabbit Recombinant Monoclonal VAMP2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 15 publications.

Images

Western blot - Anti-VAMP2 antibody [EPR12790] (AB181869), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected
Rat	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/150	Notes For unpurified use at 1/70.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10000 - 1/50000	Notes -
Species Rat	Dilution info 1/10000 - 1/50000	Notes -
Species Human	Dilution info 1/10000 - 1/50000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250	Notes For unpurified use at 1/500.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes For unpurified use at 1/150. Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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3 products for Alternative Product

Target data

See full target information VAMP2

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles (By similarity) (PubMed:30929742). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.

Alternative names

SYB2, VAMP2, Vesicle-associated membrane protein 2, VAMP-2, Synaptobrevin-2

Recommended products

Rabbit Recombinant Monoclonal VAMP2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 15 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR12790
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Vesicle-associated membrane protein 2 (VAMP2) also known as synaptobrevin 2 is a member of the SNARE protein family with a molecular mass of approximately 13 kDa. VAMP2 plays an important role in vesicle trafficking and membrane fusion particularly in the nervous system. It is prominently expressed in the brain where it facilitates neurotransmitter release by forming SNARE complexes essential for synaptic vesicle fusion with the presynaptic membrane.

Biological function summary

VAMP2 interacts with other SNARE proteins such as synaptosomal-associated protein 25 (SNAP-25) and syntaxin-1 to form stable complexes necessary for synaptic vesicle docking and exocytosis. As part of the SNARE complex VAMP2 contributes to the precise regulation of neurotransmitter release in neurons which is critical for synaptic transmission and plasticity. Its function is vital for the communication between neurons and proper brain function.

Pathways

VAMP2 plays a significant role in the synaptic vesicle cycle and neurotransmitter release pathways. It is central to the exocytosis pathway where it interacts with Rab3a a small GTPase that regulates vesicle docking. By participating in these pathways VAMP2 ensures efficient neurotransmission and contributes to neuronal communication processes. VAMP2's interaction with related proteins like SNAP-25 and syntaxin-1 highlights its importance in these synaptic processes.

Associated diseases and disorders

VAMP2 has associations with conditions such as epilepsy and neurodegenerative diseases. Alterations in VAMP2 expression or function can disrupt normal neurotransmitter release impacting synaptic function and leading to neurological symptoms. VAMP2's relationship with SNAP-25 and syntaxin-1 further implicates it in these disorders as these proteins collectively regulate neural communication. Understanding VAMP2's role in these illnesses aids in the development of potential therapeutic strategies targeting SNARE complex dysfunctions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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12 product images

Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/10000 dilution
All lanes: Rat heart lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 13 kDa
Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (ab181869)
ab181869 staining VAMP2 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Negative control 1:PBS only.
Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (ab181869)
Immunofluorescent analysis of U87-MG cells (4% paraformaldehyde-fixed) labeling VAMP2 with ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with Dapi.
Flow Cytometry (Intracellular) - Anti-VAMP2 antibody [EPR12790] (ab181869)
Intracellular Flow Cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde labeling VAMP2 with unpurified ab181869 at 1/150 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.
Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (ab181869)
ab181869 (purified) at 1/150 immunoprecipitating VAMP2 in Human cerebellum whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used intead of Anti-Rab11A antibody [EPR7587(B)] ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (ab181869)
Predicted band size: 13 kDa
Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution
All lanes: Mouse brain lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 13 kDa, 36 kDa
Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (ab181869)
Western blot analysis of immunoprecipitation pellet from Human cerebellum lysate immunoprecipitated using ab181869 at 1/70 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] (ab181869)
Predicted band size: 13 kDa
Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution
All lanes: Human cerebellum lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 13 kDa
Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869)
All lanes: Western blot - Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilution
Lane 1: Human fetal brain lysate at 10 µg
Lane 2: Human cerebellum lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 13 kDa
Flow Cytometry (Intracellular) - Anti-VAMP2 antibody [EPR12790] (ab181869)
Intracellular Flow Cytometry analysis of SH-SY5Y cells labelling VAMP2 with purified ab181869 at 1/100 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (ab181869)
Immunocytochemistry/Immunofluorescence analysis of U87-MG (human glioblastoma) cells labelling VAMP2 with purified ab181869 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (ab181869)
Immunofluorescent analysis of SH-SYSY cells (human cell line) (4% paraformaldehyde-fixed) labeling VAMP2 with unpurified ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor^®488) at 1/200 dilution and counterstained with DAPI.