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AB215721

Anti-VAMP2 antibody [EPR20818]

5

(3 Reviews)

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(2 Publications)

Rabbit Recombinant Monoclonal VAMP2 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

Syb2, Vamp2, Vesicle-associated membrane protein 2, VAMP-2, Synaptobrevin-2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] (AB215721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] (AB215721)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human kidney. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] (AB215721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] (AB215721)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat cerebrum. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] (AB215721)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] (AB215721)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse cerebrum. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-VAMP2 antibody [EPR20818] (AB215721)
  • IP

Supplier Data

Immunoprecipitation - Anti-VAMP2 antibody [EPR20818] (AB215721)

VAMP2 was immunoprecipitated from 0.35 mg mouse brain lysate with ab215721 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215721 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : Mouse brain lysate 10 μg (Input).

Lane 2 : ab215721 IP in mouse brain lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab215721 in mouse brain lysate.

Exposure time : 1 second.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-VAMP2 antibody [EPR20818] (ab215721)

Predicted band size: 13 kDa

Observed band size: 18 kDa

false

Western blot - Anti-VAMP2 antibody [EPR20818] (AB215721)
  • WB

Supplier Data

Western blot - Anti-VAMP2 antibody [EPR20818] (AB215721)

Exposure times : Lanes 1,3-6 : 3 minutes; Lanes 2,7 : 1 second.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VAMP2 antibody [EPR20818] (ab215721) at 1/1000 dilution

Lane 1:

Rat heart lysate at 20 µg

Lane 2:

Human fetal brain lysate at 20 µg

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line), whole cell lysate at 20 µg

Lane 4:

SH-SY5Y (human neuroblastoma cell line from bone marrow), whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast cell line), whole cell lysate at 20 µg

Lane 6:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus), whole cell lysate at 20 µg

Lane 7:

Human cerebellum lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

Observed band size: 18 kDa

true

Western blot - Anti-VAMP2 antibody [EPR20818] (AB215721)
  • WB

Supplier Data

Western blot - Anti-VAMP2 antibody [EPR20818] (AB215721)

Exposure times : Lanes 1-3, 7-8 : 1 second; Lanes 4-5 : 3 minutes.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VAMP2 antibody [EPR20818] (ab215721) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Mouse hippocampus lysate at 20 µg

Lane 3:

Mouse cerebellum lysate at 20 µg

Lane 4:

Neuro-2a (mouse neuroblastoma cell line), whole cell lysate at 20 µg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma cell line), whole cell lysate at 20 µg

Lane 6:

C6 (rat glial tumor cell line), whole cell lysate at 20 µg

Lane 7:

Rat cerebellum lysate at 20 µg

Lane 8:

Rat hippocampus lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 13 kDa

Observed band size: 18 kDa

true

  • Carrier free

    Anti-VAMP2 antibody [EPR20818] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20818

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vesicle-associated membrane protein 2 (VAMP2) also known as synaptobrevin 2 is a member of the SNARE protein family with a molecular mass of approximately 13 kDa. VAMP2 plays an important role in vesicle trafficking and membrane fusion particularly in the nervous system. It is prominently expressed in the brain where it facilitates neurotransmitter release by forming SNARE complexes essential for synaptic vesicle fusion with the presynaptic membrane.
Biological function summary

VAMP2 interacts with other SNARE proteins such as synaptosomal-associated protein 25 (SNAP-25) and syntaxin-1 to form stable complexes necessary for synaptic vesicle docking and exocytosis. As part of the SNARE complex VAMP2 contributes to the precise regulation of neurotransmitter release in neurons which is critical for synaptic transmission and plasticity. Its function is vital for the communication between neurons and proper brain function.

Pathways

VAMP2 plays a significant role in the synaptic vesicle cycle and neurotransmitter release pathways. It is central to the exocytosis pathway where it interacts with Rab3a a small GTPase that regulates vesicle docking. By participating in these pathways VAMP2 ensures efficient neurotransmission and contributes to neuronal communication processes. VAMP2's interaction with related proteins like SNAP-25 and syntaxin-1 highlights its importance in these synaptic processes.

VAMP2 has associations with conditions such as epilepsy and neurodegenerative diseases. Alterations in VAMP2 expression or function can disrupt normal neurotransmitter release impacting synaptic function and leading to neurological symptoms. VAMP2's relationship with SNAP-25 and syntaxin-1 further implicates it in these disorders as these proteins collectively regulate neural communication. Understanding VAMP2's role in these illnesses aids in the development of potential therapeutic strategies targeting SNARE complex dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the targeting and/or fusion of transport vesicles to their target membrane (PubMed : 9430681). Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles (PubMed : 15475946). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (By similarity).
See full target information Vamp2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature biotechnology : PubMed41028831

2025

Derivation of embryonic stem cells across avian species.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Chen,Zheng Guo,Xinyi Tong,Xizi Wang,Xugeng Liu,Hiroki Nagai,Ping Wu,Jiayi Lu,David Huss,Martin Tran,Carol Readhead,Christina Wu,Lin Cao,Yixin Huang,Zhaohan Zeng,Fan Feng,Nima Adhami,Sirjan Mor,Rusty Lansford,Cheng-Ming Chuong,Guojun Sheng,Carlos Lois,Qi-Long Ying

Frontiers in endocrinology 11:29 PubMed32117057

2020

Stathmin-2 Mediates Glucagon Secretion From Pancreatic α-Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Farzad Asadi,Savita Dhanvantari
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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