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AB216044

Anti-VAMP5 antibody

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(1 Publication)

Rabbit Polyclonal VAMP5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VAMP5.

View Alternative Names

HSPC191, VAMP5, Vesicle-associated membrane protein 5, VAMP-5, Myobrevin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP5 antibody (AB216044)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP5 antibody (AB216044)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling VAMP5 with ab216044 at 1/100 dilution.

Western blot - Anti-VAMP5 antibody (AB216044)
  • WB

Supplier Data

Western blot - Anti-VAMP5 antibody (AB216044)

Gel : 12% SDS-PAGE.

All lanes:

Western blot - Anti-VAMP5 antibody (ab216044) at 1/650 dilution

All lanes:

Human prostate lysate at 40 µg

Secondary

All lanes:

Goat anti rabbit IgG at 1/8000 dilution

Predicted band size: 12 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VAMP5.

O95183

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAMP5 also known as Myobrevin belongs to the vesicle-associated membrane protein family and plays an important role in vesicular transport. This protein is approximately 11 kDa in mass and primarily locates in muscle tissues. VAMP5 is essential in neurotransmitter release and exocytosis processes assisting the fusion of vesicles with target membranes to deliver contained cargo to specific destinations inside the cell. Its expression is higher in skeletal and cardiac muscles reflecting its specialized functions in these areas.
Biological function summary

Vesicle fusion and neurotransmission heavily depend on VAMP5 as it forms part of the SNARE complex. It binds with other SNARE proteins such as SNAP25 and syntaxin to facilitate the docking and fusion of vesicles at the plasma membrane. The correct function of VAMP5 ensures proper communication between cells helping regulate muscle contraction and relaxation. VAMP5's role in muscular tissues emphasizes its importance in maintaining normal physiological processes in these tissues and organs.

Pathways

VAMP5 operates within the exocytosis pathway and is central to synaptic vesicle fusion. It works in concert with other proteins of the SNARE complex to mediate vesicle targeting to the membrane important for neurotransmitter release at synaptic junctions. Additionally it interacts with synaptotagmins which act as sensors for calcium ions further ensuring precise timing and regulation of vesicle fusion during neurotransmission. These interactions illustrate the integration of VAMP5 in critical pathways responsible for neural and muscular functions.

Muscle dysfunction and certain neuropathic conditions relate to VAMP5 abnormalities. Mutations or misregulation in VAMP5 have been implicated in disorders such as myopathy where muscle weakness and degeneration occur. Its connection to the SNARE complex including proteins like SNAP25 highlights the potential for disruptions in this network to contribute to disease pathologies. Understanding VAMP5's role in these disorders can provide insights into therapeutic strategies targeting these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May participate in trafficking events that are associated with myogenesis, such as myoblast fusion and/or GLUT4 trafficking.
See full target information VAMP5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed36078115

2022

The Degradation of TMEM166 by Autophagy Promotes AMPK Activation to Protect SH-SY5Y Cells Exposed to MPP.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaozhong Liao,Zunshuang Gong,Zhe Wang,Weiyan Yang,Wenjing Liu,Lin Hou,Xiaokun Liu,Junnan Hua,Bin Wang,Ning Li
View all publications
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Product promise

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