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AB244398

Anti-VANGL1 antibody

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Rabbit Polyclonal VANGL1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human VANGL1 aa 1-150.

View Alternative Names

STB2, VANGL1, Vang-like protein 1, Loop-tail protein 2 homolog, Strabismus 2, Van Gogh-like protein 1, LPP2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody (AB244398)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody (AB244398)

PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for VANGL1 (green) using ab244398 at 4 μg/ml in ICC/IF.

Western blot - Anti-VANGL1 antibody (AB244398)
  • WB

Unknown

Western blot - Anti-VANGL1 antibody (AB244398)

All lanes:

Western blot - Anti-VANGL1 antibody (ab244398) at 0.4 µg/mL

Lane 1:

Control siRNA transfected MCF7 (Human breast adenocarcinoma cell line) cell lysate

Lane 2:

siRNA probe #1 transfected MCF7 cell lysate

Lane 3:

siRNA probe #2 transfected MCF7 cell lysate

Predicted band size: 60 kDa

false

Western blot - Anti-VANGL1 antibody (AB244398)
  • WB

Unknown

Western blot - Anti-VANGL1 antibody (AB244398)

All lanes:

Western blot - Anti-VANGL1 antibody (ab244398) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

VANGL1 overexpression HEK-293T cell lysate

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human VANGL1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q8TAA9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VANGL1 also known as Van Gogh-Like Protein 1 plays a mechanical role in cell biology related to polarized cell movement and development. It has a molecular mass of approximately 65 kDa. VANGL1 is expressed in various tissues with significant presence in the kidneys lungs and brain. It localizes to the cell membrane where it participates in the establishment of cell polarity.
Biological function summary

VANGL1 is an essential component of the planar cell polarity (PCP) signaling pathway where it guides cell orientation and tissue architecture. This protein is part of a larger protein complex that includes Prickle and Fluorescent in situ hybridization (Fzd) receptors. VANGL1's interactions in this complex facilitate cellular orientation playing a central role in the morphogenesis of epithelial tissues.

Pathways

Researchers have linked VANGL1 to the Wnt/PCP and non-canonical Wnt signaling pathways. These pathways are important for the regulation of cytoskeletal dynamics and cell motility. VANGL1 interacts with proteins such as the Dishevelled protein family and CELSR1 which are important for signal transduction within these pathways. Proper VANGL1 function ensures accurate cell positioning and movement which are key for embryonic development and organogenesis.

VANGL1 has been implicated in conditions such as neural tube defects and colorectal cancer. In neural tube defects mutations in VANGL1 may disrupt normal cell polarity processes interfering with neural tube closure. Similarly aberrant VANGL1 expression and its interactions with other proteins like KIF7 in the PCP pathway have been associated with the progression of colorectal cancer. Understanding VANGL1's interactions and mutations can provide insights into mechanisms underlying these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information VANGL1
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Product promise

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