JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB176575

Anti-VANGL1 antibody [EPR12415]

4

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal VANGL1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

STB2, VANGL1, Vang-like protein 1, Loop-tail protein 2 homolog, Strabismus 2, Van Gogh-like protein 1, LPP2

1 Images
Western blot - Anti-VANGL1 antibody [EPR12415] (AB176575)
  • WB

Supplier Data

Western blot - Anti-VANGL1 antibody [EPR12415] (AB176575)

All lanes:

Western blot - Anti-VANGL1 antibody [EPR12415] (ab176575) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

MOLT4 cell lysate at 10 µg

Predicted band size: 60 kDa

true

  • Carrier free

    Anti-VANGL1 antibody [EPR12415] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12415

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VANGL1 also known as Van Gogh-Like Protein 1 plays a mechanical role in cell biology related to polarized cell movement and development. It has a molecular mass of approximately 65 kDa. VANGL1 is expressed in various tissues with significant presence in the kidneys lungs and brain. It localizes to the cell membrane where it participates in the establishment of cell polarity.
Biological function summary

VANGL1 is an essential component of the planar cell polarity (PCP) signaling pathway where it guides cell orientation and tissue architecture. This protein is part of a larger protein complex that includes Prickle and Fluorescent in situ hybridization (Fzd) receptors. VANGL1's interactions in this complex facilitate cellular orientation playing a central role in the morphogenesis of epithelial tissues.

Pathways

Researchers have linked VANGL1 to the Wnt/PCP and non-canonical Wnt signaling pathways. These pathways are important for the regulation of cytoskeletal dynamics and cell motility. VANGL1 interacts with proteins such as the Dishevelled protein family and CELSR1 which are important for signal transduction within these pathways. Proper VANGL1 function ensures accurate cell positioning and movement which are key for embryonic development and organogenesis.

VANGL1 has been implicated in conditions such as neural tube defects and colorectal cancer. In neural tube defects mutations in VANGL1 may disrupt normal cell polarity processes interfering with neural tube closure. Similarly aberrant VANGL1 expression and its interactions with other proteins like KIF7 in the PCP pathway have been associated with the progression of colorectal cancer. Understanding VANGL1's interactions and mutations can provide insights into mechanisms underlying these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information VANGL1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:752 PubMed39419978

2024

PAK6 rescues pathogenic LRRK2-mediated ciliogenesis and centrosomal cohesion defects in a mutation-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Iannotta,Rachel Fasiczka,Giulia Favetta,Yibo Zhao,Elena Giusto,Elena Dall'Ara,Jianning Wei,Franz Y Ho,Claudia Ciriani,Susanna Cogo,Isabella Tessari,Ciro Iaccarino,Maxime Liberelle,Luigi Bubacco,Jean-Marc Taymans,Claudia Manzoni,Arjan Kortholt,Laura Civiero,Sabine Hilfiker,Michael L Lu,Elisa Greggio

The Kaohsiung journal of medical sciences 39:576-586 PubMed36825520

2023

Euchromatic histone lysine methyltransferase 2 facilitates radioresistance in prostate cancer by repressing endoplasmic reticulum protein 29 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Chao Huang,Jun Huang,Chang-Kun Huang,Yi Hou,Bin Zhu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB242311

Anti-VANGL1 antibody [CL0241]

Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody [CL0241] (AB242311)

  • 0
  • 0
Primary Antibodies

AB244398

Anti-VANGL1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-VANGL1 antibody (AB244398)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB109529

Anti-CD82 antibody [EPR4112]

RabMAb|Recombinant

Western blot - Anti-CD82 antibody [EPR4112] (AB109529)

  • 0
  • 0
Primary Antibodies

AB184224

Anti-Strep-tag II antibody [GT661]

Lab Essentials

Western blot - Anti-Strep-tag II antibody [GT661] (AB184224)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB252881

Anti-GFP antibody [3H9]

BOND RX™ Validated|Recombinant|Lab Essentials|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] (AB252881)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com